Third Generation Sequencing. Today Illumina – Solexa sequencing technology 454 Life sciences – 454 sequencer Applied Biosystem – SOLiD system Tomorrow.

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Presentation transcript:

Third Generation Sequencing

Today Illumina – Solexa sequencing technology 454 Life sciences – 454 sequencer Applied Biosystem – SOLiD system Tomorrow Helico – Genetic Analysis Systems Pacific Bioscience – Singel Molecule Real Time System Oxford Nanopore – Nanopore sequencing Life technologies - Ion Torrent

Helico – Genetic Analysis Systems Helico – True Singel Molecule sequencer Pacific Bioscience – Singel Molecule Real Time System Oxford Nanopore – Nanopore sequencing Life technologies - Ion Torrent Helicos® Genetic Analysis System Performance Specifications SEQUENCING PERFORMANCE AS OF MARCH 1, 2010 Routine Usage Specifications 1 Strand Output 600M to 1B usable strands per run 12 to 20M usable strands per channel Sample Throughput Multiplexing capabilities up to 96 samples per channel with molecular barcoding. Up to 4,800 samples per run. Total Output 21 to 35 Gigabases per run 420 to 700 Megabases per channel Read Length 25 to 55 bases in length 35 average length Accuracy>99.995% consensus accuracy at >20X coverage Raw Error Rate Substitution 0.2% Insertion 1.5% Deletion 3.0% Template Size20 to 8,000 bases % GC Coverage<20% coverage variation for % GC (E. coli) Instrument Performance / Configuration Flow Cells 1 flow cell or 2 flow cells processed simultaneously 25 independent channels per flow cell Sequencer Throughput > 1 Gigabases per hour 2