Practical of the First and Second Experiments

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Practical of the First and Second Experiments Separation of Plasma and Serum from Whole Blood and Separation of Main Proteins in Plasma and Serum Practical of the First and Second Experiments

Objectives Plasma Separation, and calculation of percentage Serum Separation, and calculation of percentage Plasma Proteins separation and detection

Separation of Plasma and Serum from Whole Blood 1-Plasma Separation Centrifuge at 3000 rpm for 10 minutes Record the volume of the Whole Blood (V1) Record the volume of the plasma (V2) Record the volume of the RBC (V3) and Calculate the percentage and compare it to normal Plasma Red Blood Cell

Results Component Total Volume Percentage (%) 1 Whole Blood V1= 2 Plasma V2= 3 RBCs V3=

Separation of Plasma and Serum from Whole Blood 2-Serum Separation Centrifuge at 3000 rpm for 10 minutes Record the volume of the Whole Blood (V1) Record the volume of the Serum (V2) Record the volume of the RBC (V3) and Calculate the percentage and compare it to normal

Results Component Total Volume Percentage (%) 1 Whole Blood V1= 2 Serum V2= 3 RBCs V3=

Separation of Main Proteins in Plasma and Serum There are many method for plasma proteins separation In this lab we will use salting out and then centrifugation Fibrinogen need a low concentration of salt to be separated Why? After that we will use different method for protein detection: Clotting test (to determine the presence of Fibrinogen) Biuret Test (purple color) Heat Coagulation Test

Method Serum Plasma Separation of Globulin from Albumin Heat coagulation Test Salting out Globulin Biuret test Heat Coagulation Test Plasma Separation of Fibrinogen from other proteins Albumin+ Globulin Detect the presence of Fibrinogen Fibrinogen Biuret test Clotting Test