The Jackson Laboratory Bar Harbor, ME Genetic tools and resources for orofacial clefting research PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators:

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Presentation transcript:

The Jackson Laboratory Bar Harbor, ME Genetic tools and resources for orofacial clefting research PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators: Tom Gridley, Ph.D.

FaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution ~ 50 strains in the JAX FaceBase Repository most are public, some still private, some imported and under development for distribution new knockout strains - most on B6;129 background – Bambi, Osr1, Osr2, Jag2, Snai1 and Snai2, Gad1, Slc32a1, Tgfbr2, Wnt9b new Cre strains – Osr2 (B6.129S1), Tgfb3 (STOCK) new ENU induced mutant strain (on FVB.A) – Irf6 Genetic Resource Science

FaceBase Cre Driver Project: Current progress and future plans Goal: To generate 15 Cre driver strains for orofacial clefting research Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63- CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knockin). Current progress: – All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete – 2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges). Injections to be scheduled – 2 enhancer elements are ready for targeting into JM8A3 ES cells (this month) – Future enhancer cloning will be very simple and efficient – On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010 Future plans – Continue to develop approach as better technology emerges (e.g. 3’ UTR knock-in): proof of principle using same promoter? – Choose new drivers from data generated in other FaceBase projects and in consultation with mouse subcommittee members