Structure and Function of Proteins Lecturer: Dr. Ora Furman 26-27 Oct 2009 Winter 2009/2010 Teaching Assistants: Sivan Pearl Miriam Oxsman.

Slides:

Advertisements

Similar presentations

Antigens & HLA Dr.Ghada Boghdadi.

Advertisements

Antigen Presentation K.J. Goodrum Department of Biomedical Sciences Ohio University 2005.

The MHC complex: genetics, function and disease association

Introduction to Immunology

Transplantation Immunology1 Transplantation: Chapter 17 You are not responsible for: Immunosuppressive therapies Clinical aspects of specific organ transplants.

Lecture 4 January 30, 2006 End of Chapter 3: codominance through complementation.

Lecture 3 clinical immunology Antigen Presenting Cells

MHC Polymorphism Ole Lund. Objectives What is HLA polymorphism? What is it good for? How does it make life difficult for vaccine design? Definition of.

Antigen presentation to T lymphocytes Chapter 5. Objectives Explain and illustrate the mechanisms of antigen processing for presentation on –MHC I –MHC.

MHC Polymorphism. MHC Class I pathway Figure by Eric A.J. Reits.

“Inverse Kinematics” The Loop Closure Problem in Biology Barak Raveh Dan Halperin Course in Structural Bioinformatics Spring 2006.

Major Histocompatibility Complex Chapter 7. MHC Major Histocompatibility Complex –Cluster of genes found in all mammals –Its products play role in discriminating.

T Cell Receptor (TCR) & MHC Complexes-Antigen Presentation

Major histocompatibility complex (MHC) and T cell receptors

Chapter 8 Major Histocompatibility Complex Dr. Capers

1101/20/98 Major Histocompatability Complex Molecular chaparones Hugh B. Fackrell.

The Major Histocompatibility Complex And Antigen Presentation

Cytotoxic CD8 T cells recognize antigen presented by class I MHC proteins Figure 1-30 CD8 is a T cell coreceptor that binds to class I MHC molecules. CD8.

How Cells of the Immune System “See” and Respond to Antigen

Major Histocompatibility Complex Molecules

Institute of Immunology, ZJU

Structure and Function of Proteins Lecturer: Dr. Ora Furman Oct 2009 Winter 2009/10 Teaching Assistants: Miraim Oxsman Sivan Pearl.

INSTITUTE FOR IMMUNOBIOLOGY Major Histocompatibility Complex MHC Department of Immunology Fudan University Bo GAO, Ph.D

Chapter 7 Major Histocomptibility Complex (MHC).

INTRA Proteasome TAP MHC I Golgi Calnexin Calreticulin Tapasin CD8 T C EXTRA Li MHC II Golgi Vesicle CLIP HLA-DM CD4 T H Summary.

Ad5 expressing Gag, Pol and Nef Immune responses in control of HIV replication non-human primates // SIV extrapolated to humans // HIV Prospects for.

Major Histocompatibility Complex and Transplantation Major histocompatibility complex (MHC) proteins were discovered for the first time with the advent.

Strong CTL-mediated allogeneic reactions have been reported between HLA-B*4402 and –B*4403 alleles. These alleles differ only by a single residue located.

Cell Mediated Immunity (CMI)

Fe A. Bartolome, MD, FPASMAP Department of Microbiology Our Lady of Fatima University.

MAJOR HISTOCOMPATIBILITY COMPLEX. MAJOR HISTOCOMPATIBILITY COMPLEX (MHC): Is a segment of the short arm (p) of chromosome 6 containing several genes These.

Chapter 8- MHC’s & Antigen Presentation

Cell Mediated Immunity

How T cells recognize antigen

T Cell Receptor (TCR) & MHC Complexes-Antigen Presentation Pin Ling ( 凌斌 ), Ph.D. ext 5632; References: 1. Abbas, A, K. et.al,

Major Histocompatibility complex OR MHC MHC The principal function of T cells are : The principal function of T cells are : Defense against intracellular.

Transplantation Immunology Unit College of Medicine

Cytokines are a diverse group of non- antibody proteins released by cells that act as intercellular mediators, especially in immune processes.

Specific Defenses of the Host Part 2 (acquired or adaptive immunity)

Lecture 19 November 16 th 2010 Quiz 2 scheduled for November 23 rd not November 18th.

Sequence/Structure Variability in the Major Histocompatibility Complex Protein Class I alpha chain. Errol Archibold, Mark Maloney and Rafael Tosado. BioQuest.

T Cells:Adaptive/Specific Immunity Immunity Immunity.

Major Histocompatibility Complex (MHC) Human Leukocyte Antigen (HLA)

Major Histocompatibility Complex 1. To give an overview of the role of the major histocompatibility complex in immune responses. 2. Genetics of the class.

Antigen Processing and Presentation

MHC March 24, :00-12:00.

T cell receptor & MHC complexes-Antigen presentation

Chapter 7. 주조직적합성 복합체 1. 주조직적합성 복합체 (MHC)의 발견 2. MHC 분자의 구조

T Cell Receptor (TCR) & MHC Complexes-Antigen Presentation

Major Histocompatibility Complex

Intracellular Pathogens Extracellular Pathogens

The Major Histocompatibility Complex (MHC)

Major Histocompatibility complex OR

Major Histocompatibility Complex

Chapter 8 Major Histocompatibility Complex

Figure 1 CTLA-4 and PD-1–PD-L1 immune checkpoints

The Major Histocompatibility Complex (MHC)

Major Histocompatibility complex OR

بنام خداي زيبائيها.

Key amino acid positions identified by association analysis are highlighted in three-dimensional ribbon models for the HLA-DR, HLA-DQ, HLA-A and HLA-DP.

Ligand Docking to MHC Class I Molecules

The Structure of HLA-DM, the Peptide Exchange Catalyst that Loads Antigen onto Class II MHC Molecules during Antigen Presentation Lidia Mosyak, Dennis.

Figure 3 Hypothetical mechanisms of smoking-associated

Jorge R. Oksenberg, Lisa F. Barcellos, Bruce A. C. Cree, Sergio E

Volume 17, Issue 12, Pages (December 2009)

The Major Histocompatibility Complex (MHC)

PU.1 Takes Control of the Dendritic Cell Lineage

Human Leukocyte Antigen (HLA)

Fig. 3 Conditionally expressed CAR using Notch as a signal induction and response pathway system. Conditionally expressed CAR using Notch as a signal induction.

Diagrammatic view of the locations of genes encoding classical HLA class I (HLA-A, -B, -C, -E, -F, and -G) and class II (DR, DQ, DP, and DM) molecules.

Presentation transcript:

Structure and Function of Proteins Lecturer: Dr. Ora Furman Oct 2009 Winter 2009/2010 Teaching Assistants: Sivan Pearl Miriam Oxsman

The HLA System The HLA System (i)  HLA: Human Leukocyte Antigen  This human version of MHC molecules presents pathogen-derived peptides to T-cells.  Immune HLA genes: oClass I (HLA-A/B/C) Expressed by most somatic cells. Used for cell-to-T-cells communication. oClass II (HLA-D) Expressed by B-cells, activated T-cells, MΦ, DCs and thymic endothelium. Used for communication among cells of the immune system (T-helper cells stimulation).

The HLA System The HLA System (ii)  HLA genes are extremely polymorphic.  Class I and II differ in structure and function. # of different proteinsHLA-I allele* 681HLA-A 1165HLA-B 431HLA-C * Adopted from:

The HLA System The HLA System (iii) Class I vs. class II structure: Adopted from: Klein et. al, N Engl J Med. (2000);343(10):702-9.

The HLA System The HLA System (iv) Adopted from: Klein et. al, N Engl J Med. (2000);343(10): (fig. 5). HLA-peptide interactions:  Class I groove accommodates 7 to 15 residues long peptides.  An HLA class I molecule has 6 pockets along the groove, 2 or 3 determine peptide specificity.  Particular allele product binds thousands of ligands.

Superimposition Adopted from: Klein et. al, N Engl J Med. (2000);343(10): (fig. 5). One molecule is rotated and translated to fit the other with minimal RMS RMS = Root Mean Square (of the distances between the atoms)

Homework I Question 1 Please send the figure by to Sivan Question 2 Rehearse some basic facts that regard interactions. You can look at the introductions of the course’ books (see course’ website for the booklist).website Look at the presentation from the 1 st lesson and try to think of all possible AA-AA interactions [Hydrophobic, ionic and H-bonds (disregard donor/acceptor issues)]. SUBMIT IN PAIRS !

Homework II Question 2 – cont. Use PDB and sPDBv to find the neighbors of positions 2 and 9 in both molecules. Elaborate on the way in which a certain trait of the peptide’s AA might effect its neighbors’ identity: compare the neighbors of position 2 in the 1 st molecule to the neighbors of position 2 in the 2 nd ; the same for position 9.