The Central Dogma of Molecular Biology DNA  RNA  Protein  Trait.

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Presentation transcript:

The Central Dogma of Molecular Biology DNA  RNA  Protein  Trait

RNA vs. DNA Study the images of the two molecules and observe their similarities & differences.

The Genetic Code How are the instructions for assembling amino acids into proteins encoded into DNA? There are 20 amino acids, but there are only four nucleotide bases in DNA How many bases correspond to an amino acid?

Codons: Triplets of Bases Example: Base triplet 3’-ACC-5’ on DNA Provides a template for 5’-UGG-3’ on mRNA UGG is the codon for the amino acid tryptophan

Central Dogma of Molecular Biology

The Central Dogma of Molecular Biology DNA  RNA  Protein  Trait Transferring genetic information into protein.

Transcription: Rewriting DNA into mRNA Enzymes add nucleotides to mRNA from 5’ to 3’ Transcription is initiated at a promoter, a sequence of DNA that signals the start of a gene

Enzymes Modify the “pre-mRNA” Enzymes attach a “cap” to the mRNA which binds the mRNA to the ribosome Enzymes attach a tail of nucleotides to the mRNA, this controls the “lifespan” of the mRNA

mRNA is Spliced Introns: Non-coding regions of DNA Exons: Coding regions of DNA

The Functional and Evolutionary Importance of Introns Some genes can encode more than one kind of polypeptide, depending on which segments are treated as exons during RNA splicing The number of different proteins an organism can produce is much greater than its number of genes Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings

Translation: The RNA directed synthesis of a polypeptide

The Genetic Code

Molecules of tRNA are not identical: –Each carries a specific amino acid on one end –Each has an anticodon on the other end; the anticodon base-pairs with a complementary codon on mRNA Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings

Translation mRNA codons are translated into a polypeptide chain

Analysis Questions Compare and contrast transcription and translation in prokaryotic and eukaryotic cells. Compare and contrast DNA replication with transcription

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