MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS Ch.Shivaprasad Assistant Professor (AGB)

Slides:



Advertisements
Similar presentations
RAPD markers Larisa Gustavsson (Garkava)
Advertisements

Polymerase Chain Reaction
Module 12 Human DNA Fingerprinting and Population Genetics p 2 + 2pq + q 2 = 1.
Lab 8: Amplification of the tPA Locus using the Polymerase Chain Reaction (PCR)
Determination of Extra-Pair Fertilization and Inbreeding Using Microsatellite Genotyping in a Captive Population of Zebra Finches Lindsay Miller, Julia.
Molecular LDT in Newborn Screening Laboratories
COMPUTER EXERCISE Design of PCR and PCR-RFLP experiments This presentation shows all steps of a PCR-RFLP experiment and is a companion of the computer.
DNA Fingerprinting and Forensic Analysis Chapter 8.
Generation and Analysis of AFLP Data
Definition of PCR Requirements for PCR PCR Process Agarose gel electrophoresis.
Lab 8: PCR (Polymerase Chain Reaction)
Introduction to DNA.
General Genetics. PCR 1.Introduce the students to the preparation of the PCR reaction. PCR 2.Examine the PCR products on agarose gel electrophoresis.
Initial Pool Generation Based on DNA Shuffling Method Eun-jeong Lee.
Bioinformatics/PCR Lab How does having a certain genetic marker affect chances of getting brain cancer?
Polymerase Chain Reaction
WORKSHOP (1) Presented by: Afsaneh Bazgir Polymerase Chain Reaction
Kingdom of Saudi Arabia King Saud University College of Applied Medical Sciences Biomedical Technology Department.
©2001 Timothy G. Standish Romans 5:17 17For if by one man’s offence death reigned by one; much more they which receive abundance of grace and of the gift.
Polymerase Chain Reaction
Polymerase Chain Reaction (PCR)
KEYS Lab Training DNA Barcoding: Identification of Species
Biotechnology. Comparative genomics also has been used to identify recently mobilized transposons in genetically diverse humans. For example, over 600.
Recombinant DNA Technology………..
By: Kelly and Kathryn PCR. What exactly is PCR? PCR stands for “polymerase chain reaction” and is a lab technique used to clone segments of DNA. Two main.
Polymerase Chain Reaction (PCR)
PCR Troubleshooting Virginia Balke
Chapter 14: DNA Amplification by Polymerase Chain Reaction.
PCR Forensics. Today’s Lab There has been an outbreak of Salmonella poisoning in the Student Union cafeteria at Stanford University cafeteria. You have.
Genotyping and association analysis of Gossypium hirsutum lines for resistance in Reniform Nematodes Megha V. Sharma*, Stella Kantartzi, David Weaver,
Got Milk? SNPs, Inheritance, and the Evolution of Lactose Tolerance.
A PCR-based Protocol for In Vitro Selection of Non-Crosshybridizing Oligonucleotides R. Deaton, J. Chen, H. Bi, M. Garzon, H. Rubin and D. H. Wood.
PCR is used in; Cloning into plasmid vectors DNA sequencing Genetic screening DNA based phylogeny Functional analysis of genes Identification of DNA fingerprints.
The polymerase chain reaction
Practical training: Test for the presence of a pathogen in a wash water potato by PCR.
The polymerase chain reaction
Advantages of STR Analysis
Crime Scene Investigator PCR Basics™
Introduction to PCR Polymerase Chain Reaction
PUC 19 5’ 3’ 5’ 3’ 5’ 3’ 5’ 3’ PCR -I pUC 19 specific primers Amplicon purification PCR -II 5’ 3’ 5’ 3’ 5’ 3’ 5’ 3’ 5’ 3’ 5’ 3’ Composite pUC 19 primers.
HRM REAL TIME PCR Presented by: Dadkhah Fahimeh SNP genotyping by HRM REAL TIME PCR.
The microbial world S. Cerevisiae (yeast) Mycobacterium tuberculosis.
Polymerase Chain Reaction (PCR). What’s the point of PCR? PCR, or the polymerase chain reaction, makes copies of a specific piece of DNA PCR allows you.
Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase.
Crime Scene Investigator PCR Basics™
Rajan sharma.  Polymerase chain reaction Is a in vitro method of enzymatic synthesis of specific DNA sequences.  This method was first time developed.
PCR Polymerase chain reaction. PCR is a method of amplifying (=copy) a target sequence of DNA.
PCR The Polymerase Chain Reaction PCR The Polymerase Chain Reaction.
PCR Polymerase Chain Reaction Parviz Fallah Stem Cell Technology Research Centre.
Introduction to PCR Polymerase Chain Reaction
Lab 8: PCR (Polymerase Chain Reaction)
Polymerase Chain Reaction
PCR Basics A review.
Random Amplified Polymorphic DNA RAPD
MOLECULAR DETECTION AND IDENTIFICATION OF POTENTIAL PROBIOTIC LACTIC ACID BACTERIA ISOLATED FROM FERMENTED OLIVES Saxami Georgia1, Panagou Efstathios2,
Today’s Title: CW: DNA manipulation – separating and probing
Polymerase Chain Reaction
BIOTECHNOLOGY BIOTECHNOLOGY: Use of living systems and organisms to develop or make useful products GENETIC ENGINEERING: Process of manipulating genes.
Polymerase Chain Reaction
Lab 8: PTC Polymerase Chain Reaction Lab
Molecular Biology lecture -Putnoky
POLYMERASE CHAIN REACTION (PCR): PRINCIPLES AND APPLICATIONS
Simulating Genetic Screening
Implications of Using the ND1 Gene as a Control Region for Real-Time PCR Analysis of Mitochondrial DNA Deletions in Human Skin  Andrew Harbottle, Kim.
Introduction to Polymerase Chain Reaction (PCR)
The polymerase chain reaction
Genomic DNA Sample Preparation
Giorgio Sirugo, Kenneth K. Kidd  The American Journal of Human Genetics 
Agarose gel electrophoresis illustrating randomly amplified polymorphic DNA (RAPD) fingerprints of the four reference strains and 12 tick isolates produced.
Agarose gel electrophoresis illustrating randomly amplified polymorphic DNA (RAPD) fingerprints of the four reference strains and 12 tick isolates produced.
Presentation transcript:

MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS Ch.Shivaprasad Assistant Professor (AGB) ILFC; C.V.Sc. Korutla,SVVU, Telangana, India

INTRODUCTION It is a layer type Big size brown colored egg Disease resistant Back yard poultry production DAHLEM RED BIRDS

OBJECTIVES  Molecular genetic characterization Dahlem Red layers using RAPD markers  To study the genetic similarity and genetic distance between full sib, half sib mated non-inbred groups

MATERIALS AND METHODS  A total of 48 birds  A total of 100 decamer primers serially numbered PDP40F to PDP115F and PDP155F to PDP 180F  Genomic DNA isolated from blood samples  Checking quality of DNA  Checking quantity of genomic DNA

RAPD-PCR ANALYSIS Amplification were carried out final volume of 12.5 μl 25 pM primer 200 μM of the dNTPs 2 mM Magnesium chloride 1.25 U Taq DNA polymerase 1.25 μl 10X PCR buffer 100 ng genomic DNA

PCR REACTION StepTemperatureDuration Initial denaturation95 o C2 minutes Cyclic denaturation94 o C1 minutes Primer annealing36 o C1 minutes 40 Cycles Primer extension72 o C2 minutes Final extension72 o C10 minutes Held at4 o CForever

Electrophoresis Visualized by UV transilluminator Gel documentation Analysis of Results by using data base software

STATISTICAL ANALYSIS The scoring and matching of bands-binary character matrix Band sharing- Nei’s method Band frequency- Raman et al. Genetics distance- Kuhnlein et al.

RESULTS Primer name Number of bands amplifiedSize range (bp) TotalPolymorphicMinimumMaximum PDP40F PDP42F PDP50F PDP54F PDP62F PDP63F PDP71F PDP72F PDP73F PDP75F PDP77F PDP78F PDP79F PDP94F PDP96F PDP103F PDP110F PDP112F PDP157F PDPF168F PDP176F Total

RAPD-PCR FINGERPRINTS Full sib-unique presence of 1866bp

Half sib-unique presence of 1788bp

Non-inbred-unique presence of 421pb

Intra-population genetic similarity based on band sharing Primer nameFull sibHalf sibNon-inbred PDP40F PDP42F PDP50F PDP54F PDP62F PDP63F PDP71F PDP72F PDP73F PDP75F PDP77F PDP78F PDP79F PDP94F PDP96F PDP103F PDP110F PDP112F PDP157F PDP168F PDP176F Mean SE

Inter-population genetic similarities based on band sharing using pooled DNA Primer name Full sib and Half sib groups Half sib and Non- inbred groups Full sib and Non-inbred groups PDP 40F PDP 42F PDP 50F PDP 54F PDP 62F PDP 63F PDP 71F PDP 72F PDP 73F PDP 75F PDP 77F PDP 78F PDP 79F PDP 94F PDP 96F PDP 103F PDP 110F PDP 112F PDP 157F PDP 168F PDP 176F Mean SE

Intra-population genetic similarities based on band frequencies Primer nameFull sibHalf sib Non-inbred PDP 40F PDP 42F PDP 50F PDP 54F PDP 62F PDP 63F PDP 71F PDP 72F PDP 73F PDP 75F PDP 77F PDP 78F PDP 79F PDP 94F PDP 96F PDP 103F PDP 110F PDP 112F PDP 157F PDP 168F PDP 176F Mean SE

Inter-population genetic similarities between groups based on band frequency Primer name Full sib and Half sib groups Half sib and Non-inbred groups Full sib Non-inbred groups PDP 40F PDP 42F PDP 50F PDP 54F PDP 62F PDP 63F PDP 71F PDP 72F PDP 73F PDP 75F PDP 77F PDP 78F PDP 79F PDP 94F PDP 96F PDP 103F PDP 110F PDP 112F PDP 157F PDP 168F PDP 176F Mean SE

GENETIC DISTANCE GroupsFull sibHalf sibNon-inbred Full sib Half sib Non-inbred

THANK YOU

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.