HISTOLOGY, IMAGE ACQUISITION AND IMAGE ANALYSIS CORE FACILITY Core Director: James Tomasek, Ph.D. Research Program Coordinator: Carol Haaksma Histologist: Jeffery Smith

HISTOLOGY CORE FACILITY 1.Automated tissue processing 2.Tissue embedding 3.Sectioning a.Paraffin b.Cryostat 4.Histochemical staining 5.Immunohistochemical staining 6.Assistance with how to prepare, section and stain tissues

HISTOLOGY CORE FACILITY (CONTINUED) Location: BMSB 519 Histologist: Jeffery Smith ext Research Program Coordinator: Carol Haaksma ext PJIs are given preference on work. Work is first come basis. If priority needed please make request to Carol Haaksma. All work is tracked in an excel database. Turnaround time is given at time of submission of work if requested, based upon size of prior projects.

IMAGE ACQUISITION AND ANALYSIS CORE FACILITY 1.Olympus Provis AX-70 Photomicroscope a.Bright field b.Epifluorescence c.Phase d.Nomarski DIC e.Q-capture digital camera and image acquisition software 2.Olympus Macro Zoom Microscope a.Bright field b.Dark field c.Fluorescence d.Image from whole retinal mount to individual cell e.Olympus digital camera and image acquisition software 3.Image Analysis Software a.Image Pro Plus b.Autoquant deconvolution

IMAGE ACQUISITION AND ANALYSIS CORE FACILITY (CONTINUED) Location: BRC 261 Training Policy: Carol Haaksma will train PI of laboratory. PI responsible for training and monitoring lab members who use facility. Contact: ext Sign up calendar available on the campus net.