DNA Deoxyribose Nucleic Acid – is the information code to make an organism and controls the activities of the cell. –Mitosis copies this code so that all the new cells gets the same information. Watson and Crick are the famous scientists that discovered what DNA looked like.

- Cell Division Shape of DNA The DNA molecule is shaped like a twisted ladder also known as a double helix.

The Structure of DNA Nucleotides: a monomer of DNA made up of a 5-carbon sugar (Deoxyribose), phosphate group and a nitrogen Base.

Four nitrogen bases –Adenine (A) –Guanine (G) –Cytosine (C) –Thymine (T) What bonds together? –Adenine = Thymine –Guanine = Cytosine

The backbone of DNA is formed by the sugar and the phosphate groups. The Nitrogen bases stick out from the sides of the backbone making up the steps of the ladder. Backbone Nucleotide Nitrogen Base

AdenineGuanine Adenine and Guanine two Have two rings both DNA and RNA Found in both DNA and RNA Memory Trick: Memory Trick: It’s Got 2 Be GAP

Uracil CytosineThymine Uracil Cytosine, Thymine, and Uracil one Have only one ring both Cytosine is in both DNA and RNA DNA only Thymine is in DNA only RNA only Uracil is in RNA only Memory Trick: Memory Trick: CUT the Pyramid

How Replication Occurs DNA Replication is carried out by a series of enzymes. –These enzymes “unzips” a molecule of DNA and allows for the formation of the new strands –DNA polymerase: is the main enzyme involved because it joins individual nucleotides to produce the DNA molecule, along with “proofreading” the new strand.

Replication of DNA Replication: is the process by which DNA is duplicated. 1. The DNA ladder breaks or unzips between the nitrogen bases. 2. Then, the new nitrogen bases pair-up and attach to each half of the ladder. 3. Results in two new DNA strands that are exact copies of the original DNA Molecules

The Role of DNA A single DNA molecule can have thousands of steps or base pairs. Arrangement of these steps form a genetic “code”. –This code determines the kind of gene that forms. –Different genes determine the different traits of an organism.

Genes: are sections of DNA on a chromosome that directs the making of specific protein. –Proteins determine our traits or characteristics Proteins are made out of amino acids, which are coded in DNA

Decoding the message First step in decoding the genetic message is to copy part of the nucleotide sequence from DNA to RNA –RNA codes information to a make proteins

RNA Ribose Nucleic Acid, is different from DNA –Different sugar –only has one strand –Has all the same bases but instead of Thymine, it has Uracil

Three types of RNA 1.m-RNA or messenger RNA that decodes the DNA into RNA and takes it out of the nucleus to a ribosome in order to make proteins.

2. r-RNA or Ribosomal RNA makes the Ribosomes where proteins are made 3. t-RNA or transfer RNA transports amino acid to the ribosome in order to make proteins.

DNARNA Nitrogen Bases A, U, G, C Sugars DeoxyriboseRibose How Many Strands DoubleSingle How Many Types 13 Location Nucleus only Nucleus and Cytoplasm Shape Double helixNo helix Produces DNA makes DNADNA make RNA Size Very largeMuch Smaller A, T, G, C

Protein Synthesis o Protein Synthesis is the process that cells use to produce protein. o it involves 2 distinct phases 1. Transcription – occurs in the nucleus involves the creation of mRNA 2. Translation – occurs in the cytoplasm at a ribosome – the protein code is “read” and the correct protein is made

Proteins are chains of amino acids. (amino acid + amino acid + amino acid = protein) The order of amino acids determines protein shape Shape determines function The DNA code consists of the order of amino acids for each protein; which comes from genes

Introns: are sequences of nucleotides that are not involved in coding for proteins Exons: are sequences of nucleotides that code for proteins.

Transcription DNA cannot leave the nucleus!!! Transcription: makes a copy of the DNA into mRNA Requires RNA Polymerase –RNA Polymerase binds to DNA and separates the DNA strands. It then use on of the strands of DNA as a template in order to assemble the strand of mRNA. –How does it now where to start? Promoters: are specific regions of DNA that tell RNA Polymerase where to start

RNA Polymerase

mRNA carries the “code” from DNA to the ribosomes

Genetic Code Proteins are made from long chains of amino acids known as polypeptides. –Each polypeptide contains a combination of any or all of the 20 different amino acids –“language” of mRNA comes from the “Genetic Code” It is read in three letter nucleotide combinations known as a codon, which codes for a specific amino acid AUG – is one codon that initiates start of polypeptide and specify methionine

Using the Genetic Code Quick lab page 303 write mRNA and Amino Acid Sequence DNA = GAC AAG TCC ACA ATC

mRNA = CUG UUC AGG UGU UAG AMINO ACIDS Leu ~ Phe~ Arg ~ Cys ~ STOP Asp ~ Cys ~ Gly ~ Leu ~ Val~

Translation The decoding of an mRNA message into a polypeptide chain (aka a protein) is the known as translation The process begins when the mRNA reaches a Ribosomes. – As each codon of the mRNA molecule moves through the Ribosome, the proper amino acid is brought to the Ribosome by a tRNA

tRNA Each tRNA carries only one kind of amino acid. – Besides the amino acid, tRNA also has a anticodon An anticodon: are three unpaired bases that are complementary to one mRNA codon

Assembling of the Protein The Ribosome only has room for two tRNA. – The ribosome forms a peptide bond between the two amino acids At the same time it breaks the bond with first tRNA and the ribosome moves down to the next codon – The polypeptide chain continues to grow until the ribosome gets to the stop codon on the mRNA and releases the polypeptide chain.

2. Protein exist ER in vesicle 3. Protein Travels to Golgi Apparatus 4. Golgi Apparatus ships it to the cell membrane 5. Protein is secreted from cell 1. Secreted proteins enters Rough ER as they are being synthesized by ribosomes

Central Dogma of Molecular Biology Happens in the nucleus Happens in the Ribosome Define on back: Replication, Transcription, Translation

Gene Mutation Mutation are any changes to your DNA Point Mutation – one nucleotide substituted for another – the fat cat ate the rat the fat car ate the rat

Frameshift Mutation frameshift mutation – a nucleotide is either inserted or deleted from a gene – all of the DNA triplets from the point of mutation onward are altered.

Deletion mutation A nucleotide is deleted from the sequence –the fat cat ate the rat the ftc ata tet her at

Insertion Mutation Adds extra nucleotides –the fat cat ate the rat the fan tca tat eth era t