Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes.

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Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes find the genes! “FIRST” generation sequencing

M.L. Metzker Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs. Ion torrent Nanopore

5 Selected Technologies. 2 nd, 3 rd, and 4 th generation sequencing. Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, Solexa - - ->Illumina

Allows both directions: “pair-end”

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NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

Ion Torrent (BIU)

Pacific Biosciences

New Generation: Template With hairpins On each end

Nanopore DNA sequencing

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