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Template preparation EE Slawson Tempel, © WUSTL

Template preparation EE Slawson Tempel, © WUSTL

Template preparation EE Slawson Tempel, © WUSTL

Template preparation EE Slawson Tempel, © WUSTL

Template preparation EE Slawson Tempel, © WUSTL

© Roche, EE Slawson Tempel, © WUSTL

EE Slawson Tempel, © WUSTL

Fragment DNA 454 – Paired end reads EE Slawson Tempel, © WUSTL

Attach adapters 454 – Paired end reads EE Slawson Tempel, © WUSTL

Ligate in a circle 454 – Paired end reads EE Slawson Tempel, © WUSTL

Digest with restriction enzyme 454 – Paired end reads EE Slawson Tempel, © WUSTL

Isolate fragments containing adapter 454 – Paired end reads EE Slawson Tempel, © WUSTL

Attach sequencing adapters and sequence 454 – Paired end reads EE Slawson Tempel, © WUSTL