أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: S IMPLE S TAIN + N EGATIVE S TAIN.

Slides:

Advertisements

Similar presentations

Microbiology Techniques

Advertisements

Prokaryotic Cell Structure and Function

Endospore Staining The name "endospore" is suggestive

Lab 13: Bacteria. Review classification within the biological world Understand characteristics used to identify microorganisms Learn how to prepare a.

381 PHT Lab #2.

Bacteria (and other small topics) The Good, The Bad, and The Ugly.

Crystal Gram’s (Primary stain) Gram’s (Primary stain) Gram’s Crystal violet Gram’s iodine Safranin Alcohol.

BACTERIAL STAINING.

COLONY MORPHOLOGY ON AGAR PLATE CULTURES

Observation of bacteria using staining procedures Simple staining Gram staining.

226 PHT Lab#2 Staining techniques

Microbiology. The study of organisms too small to be seen without magnification. –Bacteria –Viruses –Fungi –Protozoa –Helminths (worms) –algae.

GRAM STAIN PROCEDURE 1. Gather all equipment and supplies Gram stain

Do Now 1.Discuss the primary function of a microscope. 2.Define resolution and justify why electron microscopes are the best tools to use when resolving.

Gram Staining Method Biology 2 Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Inoculating Loop or Wood Splint Paper Towels.

Microscopy Compound light microscope is composed of: 1- stand 2- stage 3- substage ( condenser, diaphragm) 4- body tube (carrying lens system) 5- light.

Use and Care of Microscope, Aseptic Technique, Smears, Simple Stains

Bacteria §Cells are prokaryotic and amongst the smallest known cells ( length µm ). §No membrane bound nucleus. §Have ribosomes,but no other.

Classification of bacteria

أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: SIMPLE STAIN + N EGATIVE S TAIN.

MICROSCOPES. Microscopes Microscope : an instruments used to examine very small objects (Specimens) in microbiology. Functions : To allow us to study.

Negative Stain and Acid Fast Stain

Supplemental instruction Chattahoochee Technical College

CHAPTER 3 Observing Organisms Through a Microscope Units of Measurements Microscopy: The Instruments Preparation of Specimens.

One way to classify bacteria is by examining its morphology. Bacteria appear in many different shapes. We will examine the three basic shapes but note.

Lab. Safety, Microscopes and Colony Morphology Supplemental instruction Picture from

Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.

416PHT Lab#2 Gram’s stain Acid fast stain Spore stain

The Gram Stain In the late 1800’s, Christian Gram observed that some genera of bacteria retained a dye-Iodine complex when rinsed with alcohol, while.

226pht Lab#2 Gram staining.

Lab Exercise 4: Simple Staining and Bacterial Cell Morphology.

Microbial Ecology – From Isolation to Identification

Simple Staining and Bacterial Cell Morphology

Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique.

Simple stain Rana A.Abo-Hajjras. Simple stain The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple.

Figure 4.1 Different tools are employed to study bacteria Morphology Microscopy Staining.

Aseptic techniques, smear preparation and simple staining Lab # 2 Bio 261, Microbiology Prof. Santos.

What is a Stain A stain is a substance that adheres to a cell, giving the cell color. The presence of color gives the cells significant contrast so are.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Lab #5. Review for Practical #1 Colony morphology (pg. 19)

Microbiology Professor Sidelsky 2007

Focusing the microscope simple staining

Classification of bacteria Mrs. Dalia Kamal Eldien MSC in Microbiology Mrs. Dalia Kamal Eldien MSC in Microbiology Lecture NO: 3.

BIOLOGY & BIOTECHNOLODY DEPARTMENT.  In our laboratory, bacterial morphology (form and structure) may be examined in two ways: 1.By observing living.

MICROBIOLOGY LAB 5 BIDISHA PAUL 261-BH.

Lab 3 Goals and Objectives :

Class Four Microbiology Quiz  Quiz Number One – Open Response and Identification  Microscope  Aseptic techniques  Culture transfer techniques.

Experiment 2 Gram Stain and Acid-fast Stain.  Staining - simple stain (only one dye) - differential stain (more than one dye) G+G+ G-G- Gram stain Acid-fast.

PREPARATION OF A SMEAR GRAM STAIN ACID FAST STAIN

Microbiology Laboratory Procedures.  Microbiology = the study of microbes (bacteria, fungi, and viruses) Bacteriology = study of bacteria Virology =

Classifying and identifying Microbes. Slide preparations for bacteria A. Living preparations *Hanging drop method ** see Bacteria alive in their environment.

Gram staining Techniques. Some history Bacteria are translucent Bacteria are translucent Staining make them visible under microscope Staining make them.

*One of the most important techniques in bacteriology is the Gram stain, first described by Hans-Christian Gram in it allows the differentiation.

THE GRAM STAIN. The gram stain is the most widely used staining procedure in bacteriology It is called a differential stain it differentiates between.

GRAM STAINING. OBJECTIVES ● Describe reagents used in Gram stain & purpose of these reagents. ● Color expected of Gram Pos & Gram Neg after performing.

Simple Stains & Gram stain

Microbial staining Lab 6 Abeer Saati. Microbial staining Since bacterial organisms are so minute, it is impossible to view the organisms without compound.

Gram Staining Method Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Innoculating Loop Paper Towels.

Classifying and identifying Microbes

Lab 2: Staining Bacterial Cells

Bacterial Morphology Bacteria: are unicellular free living organisms without chlorophil having doth DNAand RNA.

بسم الله الرحمن الرحيم 140 Micro Lab 7: Gram stain.

Week 4W Review cultures: Exp 2B Lab Two & Exp 3 Lab Two

Dr. Souki MICRO 200 Cerritos College

140mic General Microbiology

Simple Staining and Bacterial Cell Morphology

SHAPE OF THE BACTERIA.

COLONY MORPHOLOGY ON AGAR PLATE CULTURES

Presentation transcript:

أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: S IMPLE S TAIN + N EGATIVE S TAIN

P URPOSE  To recognize the three basic shapes of bacterial cells.

THE THREE COMMON SHAPES OF BACTERIA :

having one of the following arrangements :  Diplococcus: a pair of cocci  Streptococcus: a chain of cocci  Tetrad: a square of 4 cocci  Sarcina: a cube of 8 cocci  Staphylococcus: cocci in irregular, often grape-like clusters

 Bacillus: a single bacillus  Streptobacillus: bacilli in chains  Coccobacillus: oval and similar to a coccus

 Vibrio: an incomplete spiral or comma-shaped  Spirillum: a thick, rigid spiral  Spirochete: a thin, flexible spiral

S IMPLE STAIN

 The simple stain is a very simple staining procedure involving only one stain.  You may choose from methylene blue, safranin, and crystal violet. S IMPLE STAIN :

1. Prepare the smear. - place a small drop of water on a clean slide. Drag the sterile inoculating needle tip through the edge of colony. - Gently spread the mixture into a circle to spread out.

S IMPLE STAIN : 2. Let the smear air dry completely.

S IMPLE STAIN : 3. Heat-Fix the smear.  Smears are heat-fixed by quickly passing the slide through a flame two or three times.  This causes the microbes to stick to the slide and not get washed off during the staining process.

S IMPLE STAIN : 4. Stain the smear.  Place the slide on a rack over the sink.  Flood the smear with stain and let it for seconds.  Rinse gently and blot dry.

S IMPLE STAIN : 5. Then, place a drop of oil directly on the stained smear.Turn the oil lens into position and fine focus to observe the cells.

RESULT

Coccus (cocci pl.)

Bacillus (Bacilli pl.)

Spirillum (Spirilli pl.)

N EGATIVE S TAIN

N EGATIVE S TAIN : 1. Place a very small drop of nigrosin near one end of a slide. 2. Remove a small amount of the culture with an inoculating loop and disperse it in the drop of stain.

N EGATIVE S TAIN : 3. Use another clean slide to spread the drop of stain containing the organism using the following technique.  

5. Allow the smear to dry without heating. 6. Observe the cells with microscope. N EGATIVE S TAIN :

RESULT Negatively Stained Bacillus Negatively Stained Cocci

Video time

Thank you أ. شروق الشهراني