Locating the Repressor Binding Sites in the Bacteriophages Presented by Sonia Sharma BNFO 301.

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Locating the Repressor Binding Sites in the Bacteriophages Presented by Sonia Sharma BNFO 301

CONFIDENTIAL2 Repressors What are They Bacteriophages when infecting the Bacteria have to make an important decision

CONFIDENTIAL 3 If Life is bestowed Lytic promoters have to be silenced Repressors come to limelight to do their job They Block the promoter’s sites for lytic pathway then proceed to Lysogeny Repressor also binds to DNA at specific sequences Found multiple times in genome

CONFIDENTIAL4 Objective is to locate those sites By Utilizing computational tools integrated in BioBIKE,- A graphical programming interface that enables biologist to approach complex research questions Upstream sequences of relevant phages

CONFIDENTIAL5 Data- available for Che12, e-lambda,L5 and Bxb1

CONFIDENTIAL6

7 Same method for L5 and Bxb1

CONFIDENTIAL8

9

Testing Time Apply the same technique to find to binding sites of some random phages Packman Shaka CONFIDENTIAL10

CONFIDENTIAL11

CONFIDENTIAL12 Concluding thought Results yet to be analyzed

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