Lab Exercise 2 Culture Media. General Purpose Media: Nutrient Broth and Nutrient Agar.

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Presentation transcript:

Lab Exercise 2 Culture Media

General Purpose Media: Nutrient Broth and Nutrient Agar

Enriched Media

Selective Media: Mycological agar

Glucose Fermentation a. Neutral pH uninoculated tube of glucose broth with phenol red pH indicator. b. Fermentation of glucose and acid production (color change from red to yellow) (acidic pH) c. Organism growth, but negative for acid production (alkaline pH) Differential Media a bc

left: no lactose fermentation right: lactose fermentation Differential Media: MacConkey Agar

Gel media (in-between liquid broth and solid agar) is used to test for motility Spreading out indicates motility

Sterilization by autoclaving: psi at 121°C for15-20 minutes

Bunsen burner flame

Aseptically pouring agar plates

Agar plates are stored upside down to prevent condensation.

All labeling is done on the bottom of the agar plate 1.Initials 2. Date (mm/dd/yy) 3. Code # or letter

Environmental sampling

Typical environmental sampling results

Microbes in the Environment. This plate was inoculated with 500 L of indoor air. Approximately how many colonies are on the plate? How many different bacterial species are here?

Microbes in the Environment. This plate was inoculated with a cotton swab that was wiped over a shower drain. Fungi usually produce large, "fuzzy" colonies (marked F). Approximately how many colonies are on the plate? How many are bacterial colonies? How many different bacterial species are here? F