Lymphocyte Development and Antigen Receptor Gene Rearrangement Chapter 8.

Slides:

Advertisements

Similar presentations

Self/Non-Self Recognition

Advertisements

Control of Eukaryotic Gene Expression. 2 Eukaryotic Gene Regulation Prokaryotic regulation is different from eukaryotic regulation. 1.Eukaryotic cells.

TODAY B CELL DEVELOPMENT.

How is antibody diversity generated? Two early theories: Germline hypothesis The genome contains many loci encoding antibody molecules. B cells express.

Lecture 9- T cell development Flow cytometry- how it works Thymic architecture and cells Thymic development I- generation of a TCR  chain Thymic development.

Methylation, Acetylation and Epigenetics

Antibody structure Heavy chain constant region determines antibody class.

Self-MHC restriction of the T cell receptor. Self-MHC restriction of T C cells R. Zinkernagel & P. Doherty.

Structure of T Cell Receptor CHO Variable region “V” Constant region “C” Hinge “H ” Alpha chain Beta chain Disulfide bridge Transmembrane region Cytoplasmic.

Introduction to C.M.I., the TCR, and T Cell Development web Abbas Chapters 7,8.

Generation of diversity in lymphocyte antigen receptors Jan. 31, Feb. 2 & 5 Chapter 4.

Outline Immunoglobulin Superfamily Antigen Recognition Members:

Structure of Class II MHC (continued) 3.Transmembrane region – stretch of hydrophobic amino acids spanning membrane 4.Cytoplasmic region – contains sites.

T Cell Development Amy Lovett-Racke, PhD Associate Professor

Generation of antibodies and T cell receptors by V(D)J recombination Lymphocyte development: generation of cells with functional and useful antigen receptors.

Antibodies and T Cell Receptor Genetics 2011

Chapter 14 B Lymphocytes. Contents  B cell receptor and B cell complex  B cell accessory molecules  B cell subpopulations  Functions of B cells 

Organization & Expression of Immunoglobulin Genes

Chapter 11 B-Cell Generation, Activation, and Differentiation.

T-cell development and differentiation. T-cell diversity is generated in the thymus The TCR is a recognition unit that looks like an arm of the BCR In.

Control of gene expression Transcriptional Post-transcriptional Epigenetics and long range control.

Lymphocyte Development & Generation of Lymphocyte Antigen Receptors Pin Ling ( 凌斌 ), Ph.D. ext 5632; References: 1. Abbas, A,

Immunology 6 Specificity 8. Specificity of immunoglobulin molecule on B cell – BCR of receptor on T cell – TCR is defined and produced before their exposition.

Eukaryotic Genome & Gene Regulation The entire genome of the eukaryotic organism is present in every cell of the organism. Although all genes are present,

Organization and Expression of Immunoglobulin Genes.

This will be covered later in the course and is presented here to provide context to understanding isotype switching. It will not to be tested in Exam.

B Cell Activation and Antibody Production Lecture 15.

V(D)J Recombination and the Role of CTCF Richard Wolfe; Xinhao Liu.

Aims Gene rearrangement and class switching of B-cell Igs.

B Cell Development Learning Objectives for Lecture 11

B-Cell Maturation, Activation, and Differentiation.

Epigenetic control of Gene Regulation Epigenetic vs genetic inheritance  Genetic inheritance due to differences in DNA sequence  Epigenetic inheritance.

The genetic basis of antibody structure

Clonal Selection. Antibody Structure Made up of 4 polypeptide chains Made up of 4 polypeptide chains –2 identical heavy chains –2 identical light chains.

Chapter 4 and 5 Ig study questions (Tu): Can you name at least four ways in which CSR and V(D)J recombination differ? What are the substrates (what genes,

REVIEW: IMPORTANT INFORMATION TO DATE ABOUT ===> B cells ANTIBODY ABOUT ===> B cells & ANTIBODY

Ig Polypeptides Are Encoded by Multiple Gene Segments LIGHT CHAIN

Chapter 9 T-cell Receptor Dr. Capers. Kuby IMMUNOLOGY Sixth Edition Chapter 9 T-Cell Receptor Copyright © 2007 by W. H. Freeman and Company Kindt Goldsby.

Rearrangement The normal process by which antibodies and T cell receptors are made.

Stages of Hematopoietic Development The hematopoietic system generates blood cells. The hematopoietic differentiation is unique because it does not require.

DIFFERENTIATION AND MATURATION OF T CELLS IN THE THYMUS.

Lecture 1: Immunogenetics Dr ; Kwanama

Farshid Yeganeh PhD. Virtually any substance can be the target of an antibody response. "Beadle and Tatum in 1941 One gene-one polypeptide hypothesis“

Chapter 13 Lymphocyte Maturation and Antigen Receptor Expression

ADAPTIVE IMMUNITY. Adaptive immunity Specific Slow during the primary response, but very fast during the secondary responses memory.

Antigen recognition in adaptive immunity Mechanism of diversity of antigen receptors Maturation and selection of B lymphocytes Maturation and selection.

Chapter 5 Organization and Expression of Immunoglobulin Genes Dr. Capers.

ANTIGEN-INDEPENDENT DEVELOPMENT

The Code of Life: Topic 4 Regulation of gene expression.

Immunoglobulin Gene Rearrangement

Chapter 8 The Development and Survival of Lymphocytes.

Gene Regulation, Part 2 Lecture 15 (cont.) Fall 2008.

Immunoglobulin Genetics

IMMUN 441 Week 4 AC Quiz Section

Control of gene expression

B cells I. Differentiation of B cells in Bone marrow II

GENE REGULATION in Eukaryotic Cells

Immunoglobulin Expression and the Role of Antigen in

Immunogenetics Lecture 3: TcR.

Antibody production and B cell differentiation

Immunoglobulins (2 of 2) Ali Al Khader, MD Faculty of Medicine

Eukaryote Gene Expression/Regulation

Epigenetics Heritable alteration of gene expression without a change in nucleotide sequence.

Epigenetics Drives RAGs to Recombination Riches

Immunoglobulins (2 of 2) Ali Al Khader, MD Faculty of Medicine

Abbas Chapter 8 Lymphocyte Development and the

Genome-Wide Epigenetics

Molecular mechanisms of IgE regulation

A Role for Epigenetics in Psoriasis: Methylated Cytosine–Guanine Sites Differentiate Lesional from Nonlesional Skin and from Normal Skin Johann E. Gudjonsson,

Antigen recognition in adaptive immunity

Presentation transcript:

Lymphocyte Development and Antigen Receptor Gene Rearrangement Chapter 8

Stages of lymphocyte maturation

Pluripotent stem cells give rise to distinct B and T lineages

Epigenetics, MicroRNAs, and Lymphocyte Development Many nuclear events in lymphocyte development are regulated by epigenetic mechanisms Epigenetics refers to mechanisms that control gene expression (as well as gene rearrangement in developing lymphocytes) that go beyond the actual sequence of DNA in individual genes The mechanisms that make genes available or unavailable in chromatin are considered to be epigenetic mechanisms including DNA methylation on certain cytosine residues that generally silences genes, post-translational modifications of the histone tails of nucleosomes (e.g., acetylation, methylation, and ubiquitination

Checkpoints in lymphocyte maturation

Positive and negative selection during lymphocyte maturation

REARRANGEMENT OF ANTIGEN RECEPTOR GENES IN B AND T LYMPHOCYTES

Germline organization of human Ig loci

Domains of Ig and TCR proteins [V(D)J Recombination]

Germline organization of human TCR loci

Diversity of antigen receptor genes

V(D)J recombination

Transcriptional regulation of Ig genes

Sequential events during V(D)J recombination

Junctional Diversity

B LYMPHOCYTE DEVELOPMENT

Stages of B cell Maturation

Ig heavy and light chain gene recombination and expression

Pre-B cell and pre-T cell receptors

B lymphocyte subsets

Co-expression of IgM and IgD

MATURATION OF T LYMPHOCYTES

Stages of T cell maturation

Maturation of T cells in the Thymus

TCR α and β chain gene recombination and expression

CD4 and CD8 expression on thymocytes and positive selection of T cells in the thymus

γδ T Lymphocytes In fetal thymuses, the first TCR gene rearrangements involve the γ and δ loci The diversity of the γδ T cell repertoire is theoretically even greater than that of the αβ T cell repertoire Paradoxically, however, the actual diversity of expressed γδ TCRs is limited because only a few of the available V, D, and J segments are used in mature γδ T cells, for unknown reasons