Torino, 03 Novembre 2010 C5.6 Technologies: A young, highly innovative biotechnology company Stefano Paravisi – Project leader.

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Torino, 03 Novembre 2010 C5.6 Technologies: A young, highly innovative biotechnology company Stefano Paravisi – Project leader

C56 Italy, Srl THE STRUCTURE Enzyme cloning Technology Process Engineering PRO.E.Sa Project The joint venture Torino, 3 Novembre 2010

C56 Italy, Srl Why C5-6 Italy in Piedmont? Throughout the territory: Universities: - Politecnico - Avogadro University Innovation poles for energies Pro.E.Sa project - Politecnico - Chemtex s.r.l Biosolar lab Torino, 3 Novembre 2010

C56 Italy, Srl The project PST Tortona Chemtex s.r.l Avogadro University -Enzymes technology experience - Available laboratories - Competences - Joint venture cooperation Torino, 3 Novembre 2010

C56 Italy, Srl Project goals Using the Unique cloning tools and Single cell genomics techniques developed by C5-6 Technologies, C5-6 Italy will find the best enzymes to fit the process of enzymatic hydrolysis of ligno-cellulosic pre-treated material. Torino, 3 Novembre 2010

C56 Italy, Srl MICRORGANISMS IDENTIFICATION AND SELECTION SETUP COCKTAIL COMPOSITION AND CONDITIONS SCALE UP Activities and objectives SINGLE ENZYMES SELECTION AND ISOLATION ANALYSIS OF MICRORGANISM SECRETED ENZYMES PATTERN ENZYMES CHARACTERIZATION PERFORMANCE EVALUATION ON PRE-TREATED MATERIAL Torino, 3 Novembre 2010

C56 Italy, Srl KEY FACTORS The project: KEY FACTORS Cost for 2° generation bioethenol - High potential of enzymes activities - Low costs of enzymes production - Selection of good enzymatic package for the pre-treated selected biomass Enzymes in ethanol production Enzymes production Torino, 3 Novembre 2010

C56 Italy, Srl Enzymes will play a key role for the success of BIOFUELS From the plant… ….to distribution ENZYMES Torino, 3 Novembre 2010