Supplemental Figure 1. Loss of Stat3 expression did not change IFN-induced chemokine levels in tumors. (A) Realtime PCR analysis of mRNA expression levels.

Slides:

Advertisements

Similar presentations

Clancy-Thompson et al., Supplemental Figure 1 A B C Supplemental Figure 1: Mice were given intravenous B16 injection on day 0. A) After bearing tumors.

Advertisements

SUPPLEMENTAL MATERIAL AND METHOD Ag-presenting CpG-activated pDCs prime Th17 cells that induce tumor regression Leslie Guéry, Juan Dubrot, Carla Lippens,

Volume 94, Issue 2, Pages (March 2014)

Volume 39, Issue 5, Pages (November 2013)

Cheng-Ming Sun, Edith Deriaud, Claude Leclerc, Richard Lo-Man Immunity

Volume 80, Issue 2, Pages (July 2011)

Volume 28, Issue 2, Pages (February 2008)

Phenformin Inhibits Myeloid-Derived Suppressor Cells and Enhances the Anti-Tumor Activity of PD-1 Blockade in Melanoma Sun Hye Kim, Man Li, Sebastian.

by Norman Nausch, Ioanna E

Inhibition of Th1 Differentiation by IL-6 Is Mediated by SOCS1

Volume 31, Issue 2, Pages (August 2009)

Naive T cells sense the cysteine protease allergen papain through protease-activated receptor 2 and propel TH2 immunity Genqing Liang, PhD, Tolga Barker,

Volume 82, Issue 1, Pages (July 2012)

Volume 32, Issue 3, Pages (March 2010)

Pak2 regulates myeloid-derived suppressor cell development in mice

Volume 5, Issue 4, Pages (April 2009)

Volume 24, Issue 5, Pages (November 2013)

Volume 29, Issue 6, Pages (December 2008)

Volume 20, Issue 4, Pages (April 2004)

Volume 6, Issue 6, Pages (March 2014)

IL-17 enhances the migration of B cells during asthma by inducing CXCL13 chemokine production in structural lung cells Roua Al-Kufaidy, MS, Alejandro.

Volume 30, Issue 4, Pages (April 2009)

Volume 39, Issue 5, Pages (November 2013)

Volume 36, Issue 1, Pages (January 2012)

Volume 11, Issue 5, Pages (November 1999)

Increased chemokine content and leukocyte infiltrate in D6-negative tumors. Increased chemokine content and leukocyte infiltrate in D6-negative tumors.

Volume 29, Issue 2, Pages (August 2008)

Volume 7, Issue 4, Pages (October 2016)

Volume 13, Issue 1, Pages (January 2008)

Volume 45, Issue 1, Pages (July 2016)

Volume 47, Issue 2, Pages e5 (August 2017)

Volume 13, Issue 12, Pages (December 2015)

Volume 41, Issue 5, Pages (November 2014)

Volume 38, Issue 6, Pages (June 2013)

IFN-γ induces TNF family ligand protein expression in vitro and in vivo. IFN-γ induces TNF family ligand protein expression in vitro and in vivo. (A and.

Tumor-Residing Batf3 Dendritic Cells Are Required for Effector T Cell Trafficking and Adoptive T Cell Therapy Stefani Spranger, Daisy Dai, Brendan Horton,

Volume 13, Issue 2, Pages (October 2015)

A Mutation in the Nlrp3 Gene Causing Inflammasome Hyperactivation Potentiates Th17 Cell-Dominant Immune Responses Guangxun Meng, Fuping Zhang, Ivan Fuss,

Pivotal Role of Dermal IL-17-Producing γδ T Cells in Skin Inflammation

C5a Negatively Regulates Toll-like Receptor 4-Induced Immune Responses

Tumor-Residing Batf3 Dendritic Cells Are Required for Effector T Cell Trafficking and Adoptive T Cell Therapy Stefani Spranger, Daisy Dai, Brendan Horton,

Volume 32, Issue 5, Pages (May 2010)

Volume 32, Issue 4, Pages (April 2010)

Volume 38, Issue 3, Pages (March 2013)

Epithelial loss of TβRII results in increased Th17 cell development.

CXCR5 expression accelerates Eμ-Tcl1 leukemogenesis and is indispensable for tumor cell recruitment to lymphoid B-cell follicles. CXCR5 expression accelerates.

Volume 6, Issue 4, Pages (October 2004)

STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation

Volume 19, Issue 9, Pages (May 2017)

Volume 34, Issue 5, Pages (May 2011)

Javed Mohammed, Andrew Ryscavage, Rolando Perez-Lorenzo, Andrew J

Volume 2, Issue 2, Pages (February 2008)

Volume 30, Issue 4, Pages (April 2009)

Microbial Colonization Drives Expansion of IL-1 Receptor 1-Expressing and IL-17- Producing γ/δ T Cells Jinyou Duan, Hachung Chung, Erin Troy, Dennis L.

Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells. Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells.

Volume 35, Issue 4, Pages (October 2011)

PD-L1 selectively marks circulating NCMs.

Differential Production of Th1- and Th2-Type Chemokines by Mouse Langerhans Cells and Splenic Dendritic Cells Hideki Fujita, Akihiko Asahina, Makoto.

PD-1 blockade results in increased expression of the chemokine receptor CXCR3. PD-1 blockade results in increased expression of the chemokine receptor.

Volume 19, Issue 4, Pages (April 2017)

CD49b+ cells from tumor-bearing mice are more prone to conversion into MDSCs compared with naive CD49b+ cells. CD49b+ cells from tumor-bearing mice are.

Inflammation Mediated by JNK in Myeloid Cells Promotes the Development of Hepatitis and Hepatocellular Carcinoma Myoung Sook Han, Tamera Barrett, Michael.

Volume 25, Issue 6, Pages (June 2017)

by Gonghua Huang, Yanyan Wang, Peter Vogel, and Hongbo Chi

Volume 13, Issue 11, Pages (December 2015)

Collapse of the Tumor Stroma is Triggered by IL-12 Induction of Fas

Combination of R848 and anti-CD200R affects activation of tumor-infiltrating myeloid cells. Combination of R848 and anti-CD200R affects activation of tumor-infiltrating.

LSECtin, expressed by B16 cells, inhibits the tumor-specific immune responses both in vivo and in vitro. LSECtin, expressed by B16 cells, inhibits the.

Endogenous Control of Immunity against Infection: Tenascin-C Regulates TLR4- Mediated Inflammation via MicroRNA-155 Anna M. Piccinini, Kim S. Midwood

Volume 2, Issue 6, Pages (December 2012)

Presentation transcript:

Supplemental Figure 1. Loss of Stat3 expression did not change IFN-induced chemokine levels in tumors. (A) Realtime PCR analysis of mRNA expression levels of CXCL9, CXCL10, CXCL11 and (B) ELISA of CXCL10 levels in tumor single-cell suspensions from B16 tumor bearing Stat3 +/+ (Stat3 f/lf ) or Stat3 -/- (CD4Cre/Stat3 f/f ) mice. A B ns

Stat3  Stat3  *** A B Supplemental Figure 2. Tumor infiltrating macrophages are expressing CXCL10. (A) Left, representative flow plot of sorted macrophages gated on CD11b + cells, (B) Real-time PCR analysis of CXCL10 mRNA levels in CD11b + F4/80 + macrophages, from B16 tumors harvested from Stat3  /  or Stat3  /  mice. F4/80 CD11b Stat3  Stat3  80.6% 79.0%

CXCR3 CD8 Stat3  Stat3  24.3% 24.4% ns Supplemental Figure 3. Ablating Stat3 resulted no significant change in CXCR3 levels in CD8 + T cells from TDLN. Left, representative flow plot of cells from TDLN in B16 tumor bearing Stat3 +/+ or Stat3 -/- mice, gated on CD3 + CD8 +. Right, quantification analysis of percentages of CXCR3 + in CD3 + CD8 + T cells. Ns, not significant.

ns * Supplemental Figure 4. Blockade of IFNγ in tumors lead to significant reduction of tumor infiltrating IFNγ-expression CD8 + T cells in absence of Stat3 expression. Flowcytometry analysis of IFNγ- expressing tumor infiltrating T cells (first gated on CD3 + CD8 + ) from B16 tumor bearing Stat3 +/+ or Stat3 -/- mice. Quantification of CD8 + IFNγ + percentages are showing on the right. PBS αIFNγ 4.39% 8.37% 3.10% 2.29% IFNγ CD8 Stat3  Stat3  PBSαIFNγ

Supplemental Figure 5. CXCL10 induced CD8 + T cell migration depends on Rho signaling but independent of Cdc42 and Rac1 signaling. (A)-(B) In vitro migration of splenic CD8 + T cells from B16-bearing Stat3 +/+ or Stat3 -/- mice toward medium alone or CXCL10 at 100ng/ml. Cells were pretreated with ROCKi (Rho kinase inhibitor, 10 μM for 15 min), CT04 (Rho A family inhibitor, 1 μg/ml for 2hrs), ML141 (Cdc42 inhibitor, 10 μM for 1hr) and NSC23766 (Rac1 inhibitor, 100 μM for 1hr) before migration assay. ** A B ns

IFNγ Stat3 IFNγ CXCL10 CD8 + T cell Myeloid cell Supplemental Figure 6. Illustration of STAT3 signaling in regulating CD8 + T cells migration toward tumors in an IFNγ/CXCL10/CXCR3- dependent manner.