Dr. Mark F. Feldlaufer Research Leader Chemicals Affecting Insect Behavior Laboratory (CAIBL) Beltsville Maryland Deployed War Fighter Protection (DWFP) Research Program 2007 DoD Pest Management Workshop photo by J. Gathany CAIBL activities

Manipulation of Arthropod Behavior For Protection of Humans Chemical Signals for Managing Insects CAIBL 4 entomologists 3 organic chemists DWFP

Master Memorandum of Understanding (MOU) – “DWFP is an initiative to develop and validate novel methods to protect US military deployed abroad from threats posed by disease-carrying insects.” “The DWFP is designed not only to encourage the rapid development of such products but to improve the capability of USDA to provide long term, innovative support to military preventive medicine.” against reformulations of existing products discovery of new products fundamental research insect olfaction

reformulation of existing product – reformulation of existing product “Development and Reformulation of IR3535-Based Repellents” (IR3535 ® - Ethyl butylacetylaminoproprionate) 10% lotion 20% pump spray 20% aerosol Supplying CAIBL with Supplying CAIBL with: ‘neat’ IR3535 blank formulations formulated compounds AVON’S SKIN SO SOFT BUG GUARD PLUS IR3535® GOALS: superior product facilitate military availability

Master Memorandum of Understanding (MOU) – “DWFP is an initiative to develop and validate novel methods to protect US military deployed abroad from threats posed by disease-carrying insects.” “The DWFP is designed not only to encourage the rapid development of such products but to improve the capability of USDA to provide long term, innovative support to military preventive medicine.” against reformulations of existing products discovery of new products fundamental research insect olfaction

BASF supplies CAIBL with chemicals from their library CAIBL confirms identity and checks purity CAIBL prepares stock solutions for testing CAIBL conducts bioassays / statistical analyses discovery of new products – discovery of new products

Aedes aegypti (Liverpool) K&D module BIOASSAY

K&D module - four TEST compounds (25nM/cm 2 ) solvent control (acetone) positive control (  -cypermethrin standard) five mosquitoes/chamber (12 reps) Category 1 – no statistical difference from solvent control “INACTIVE” Category 3 – no statistical difference from  -cypermethrin standard “ACTIVE” Category 2 – statistical difference from both compounds ranked blood fed or not blood fed compounds ranked

1174 (synthetic) 33 - discovery of new products USUHS (Uniformed Services University of the Health Sciences) Kasetsart University (Thailand)

BASF supplies CAIBL with chemicals from their library CAIBL confirms identity and checks purity CAIBL prepares stock solutions for testing CAIBL conducts bioassays / statistical analyses discovery of new products – discovery of new products USUHS (Uniformed Services University of the Health Sciences) Don Roberts John Grieco Nicole Achee

measures (i) Contact irritancy (CIA) (ii) Spatial Repellency (SRA) Ae. aegypti (Thai; Houston) Culex pipiens An. albimanus An. minimus Two additional in vitro mosquito assays

Kasetsart University (Thailand) Kanchanaburi Chaing Mai

1174 (synthetic) 33 - discovery of new products 4 K&D CIA K&D CIA SRA Chemical Optimization Dose titration (25 nM/cm2) Mode of action CostRegisterability 10 K&D CIA K&D

Master Memorandum of Understanding (MOU) – “DWFP is an initiative to develop and validate novel methods to protect US military deployed abroad from threats posed by disease-carrying insects.” “The DWFP is designed not only to encourage the rapid development of such products but to improve the capability of USDA to provide long term, innovative support to military preventive medicine.” against reformulations of existing products discovery of new products fundamental research insect olfaction

Wind Tunnel research Dr. Jerry Klun attraction human breath odors with and without CO2

Manipulation of Arthropod Behavior For Protection of Humans Chemical Signals for Managing Insects CAIBL 4 entomologists 3 organic chemists DWFP

Electrophysiology of the CPB

Antenna (Nose) Single cell recording Air Hostplant odor Dick Dickens, Principal Investigator with Meiling Webb, Support Scientist Microelectrode Sensillum Dr. J. C. Dickens, BARC, CAIBL, Beltsville, MD 2007

photo by E. Erbe

Master Memorandum of Understanding (MOU) – “DWFP is an initiative to develop and validate novel methods to protect US military deployed abroad from threats posed by disease-carrying insects.” “The DWFP is designed not only to encourage the rapid development of such products but to improve the capability of USDA to provide long term, innovative support to military preventive medicine.” against reformulations of existing products discovery of new products fundamental research insect olfaction

Chemical Affecting Insect Behavior Laboratory SCIENTISTS Jeff Aldrich Kamal Chauhan Dick Dickens Mark Feldlaufer Ashot Khrimian Jerry Klun Kevin Tran * Aijun Zhang SUPPORT STAFF John Davis Blanca Evans * Fil Guzman Sandra Lara * (s) Matt Lawrence * June Nie Deborah Smith * Meiling Webb OFFICE Julia Cypriano Kawal Sethi * * DWFP - funded