Finnish Food Safety Authority

Scientific Activities on ASF in Finland Ulla Rikula Tallinn 26 Feb 2016

1. Possible Routes of Entry into the country for African Swine Fever – Risk profile  Evira Research Reports 4/2011, in Finnish with English summary, Risk Assessment Unit (updating 2016) 2. The Effects of Structural Change in Agriculture on the Spread of Animal Disease in Finland  Evira Research Reports 3/2015, in English Risk Assessment Unit & Natural Resources Institute Finland (Luke) 3

3. Rapid risk assessment tool for animal disease risk assessment - method development project  Used when rough estimate of risk needed soon after the threat arises  emergency risk management activities  Research Group: Jonna Kyyrö, Leena Sahlström, Tapani Lyytikäinen, Risk Assessment Unit, Evira   Current Projects  Epizootic Diseases 4

4. Diagnostic methods for ASF – virus detection  Conventional PCR (Aqüero et al. 2003)  Real time PCR (Fernández-Pineiro et al. 2013)*  Conventional PCR for genotyping (presequencing PCR) SOP/CISA/ASF/GENOTYPING/1 (Bastos et al. 2003)*  Antigen ELISA (Ingenasa)  * FINAS accreditation 5

4. Diagnostic methods for ASF – antibody detection  Antibody ELISA (Ingenasa)* FINAS accreditation  Indirect immunoperoxidase test (IPT) for ASF antibodies (plates and SOP from ASF reference laboratory CISA-INIA, Madrid, Spain)  Annual participation to ASF proficiency tests since 2004  methods used fit for purpose 6

Tallinn 26 Feb 2016 Cumulative numbers of hunted/dead wb tested for ASF in (n = 138 and 171, respectively) in Finland 7

5. COST Action CA15116 COST Association Understanding and combating African Swine Fever in Europe (ASF-STOP)  Objective:  Stop ASF from spreading further in Europe and protecting the European pig industry by combating ASF through a comprehensive, multi- and interdisciplinary approach  Research objectives:  Development of knowledge on ASF-virus, diagnostics and vaccines  8

The broader the smile, the better the biosecurity! Thank you! 9 EXCELLENT GOOD ROOM FOR IMPROVEMENT POOR