StainsStains. Negative Stain Cheek Cells Without Stain.

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Presentation transcript:

StainsStains

Negative Stain

Cheek Cells Without Stain

Cheek Cells with Methylene Blue

Differential Stains: Gram Stain Color of Gram + Color of Gram – Primary stain: Crystal violet Purple Mordant: Iodine Purple Decolorizing agent: Alcohol-acetone PurpleColorless Counterstain: Safranin PurplePink

Gram Positive and Gram Negative Bacteria

Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

Capsule Stain

Figure 3.12a-c

Non Acid-Fast(A) and Acid-Fast (B)

Endospore Stain: Ziehl-Neelsen

Figure 3.12a-c

Endospore Stain

Flagella Stain

Figure 3.12a-c

Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST SPORE STAIN PRIMARY STAINCrystal violet Carbol Fuschia Malachite green MORDANTIodine Heat DECOLORIZERETOH or acetone: Cell is purple ETOH or acetone: Cell is clear Acid alcohol Water COUNTER-STAINSafranin: Cell is purple Safranin: Cell is pink Methylene Blue Safranin: Spores are green Cell is pink

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