DNA Sequencing A population of DNA fragments is generated. –One end is common to all fragments (the 5’ end of the sequencing primer). –The other end terminates.

Slides:

Advertisements

Similar presentations

Analysis of your 16s RNA. DNA sequencing Most current sequencing projects use the chain termination method –Also known as Sanger sequencing, after its.

Advertisements

Replication. N N H R O CH3 O T N N R H N H O C R N N N N H H N A G R N N N O H U.

Cycle Sequencing. Broad and Long Term Objective To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis To characterize.

DNA Sequencing How do you do it?. DNA Sequencing DNA sequencing – used to determine the actual DNA sequence of an organism. Using a computer, one can.

DNA Sequencing.

Chapter 14 The Techniques of Molecular Genetics

Chapter 10 DNA Sequencing.

16.6 – Locating and Sequencing Genes. Learning Objectives Recap how DNA probes and DNA hybridisation is used to locate specific genes. Learn how the exact.

Sanger-Coulson Dideoxynucleotide Sequencing Kwamina Bentsi-Barnes Deisy Mendoza Jennifer Aoki Lecture 10/30/00 Best printed in color for clarity.

7.1 cont’d: Sanger Sequencing SBI4UP MRS. FRANKLIN.

DNA Sequencing. * Sequencing means finding the order of nucleotides on a piece of DNA. * Nucleotide order determines amino acid order, and by extension,

Automated DNA Sequencing LECTURE 7: Biotechnology; 3 Credit hours Atta-ur-Rahman School of Applied Biosciences (ASAB) National University of Sciences and.

DNA Sequencing Today, laboratories routinely sequence the order of nucleotides in DNA. DNA sequencing is done to: Confirm the identity of genes isolated.

Analyzing your clone 1) FISH 2) “Restriction mapping” 3) Southern analysis : DNA 4) Northern analysis: RNA tells size tells which tissues or conditions.

6.3 Advanced Molecular Biological Techniques 1. Polymerase chain reaction (PCR) 2. Restriction fragment length polymorphism (RFLP) 3. DNA sequencing.

How do you identify and clone a gene of interest? Shotgun approach? Is there a better way?

A Lot More Advanced Biotechnology Tools (Part 1) Sequencing.

Chapter 14 The Techniques of Molecular Genetics

19.1 Techniques of Molecular Genetics Have Revolutionized Biology

Molecular Testing and Clinical Diagnosis

Sequencing DNA 1. Maxam & Gilbert's method (chemical cleavage) 2. Fred Sanger's method (dideoxy method) 3. AUTOMATED sequencing (dideoxy, using fluorescent.

GENE SEQUENCING. INTRODUCTION CELL The cells contain the nucleus. The chromosomes are present within the nucleus.

DNA Sequencing.

Biotechniques. DNA sequencing To determine the base sequence of DNA fragments. DNA replicated into smaller fragments incorporating fluorescent tags Fragments.

6.3 Advanced Molecular Biological Techniques 1. Polymerase chain reaction (PCR) 2. Restriction fragment length polymorphism (RFLP) 3. DNA sequencing.

Sequencing by the Sanger Dideoxynucleotide Chain Termination Method 1. Prepare replication template denature, add synthetic primer, promote annealing TAGGCGA.

Locating and sequencing genes

Biotech. Cloning a mammal PCR This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time.

Bioinformatics Computing 1 CMP 807 – Day 2 Kevin Galens.

Sanger or Dideoxy DNA Sequencing

DNA Sequencing Mimi Chen & Joanne Kim

DNA Sequencing Mimi Chen & Joanne Kim

DNA Sequencing Sanger Di-deoxy method of Sequencing Manual versus Automatic Sequencing.

DNA Sequencing Hunter Jones, Mitchell Gage. What’s the point? In a process similar to PCR, DNA sequencing uses a mixture of temperature changes, enzymes.

Automated DNA Sequencing AP Biology Fall Automated DNA Sequencing  Currently, laboratories use automated DNA sequencing to determine the unknown.

핵산 염기서열 분석(DNA SEQUENCING)

Topic Cloning and analyzing oxalate degrading enzymes to see if they dissolve kidney stones with Dr. VanWert.

DNA Sequencing First generation techniques

DNA sequencing DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology.

Restriction Fragments and Mapping

Genomics Sequencing genomes.

DNA Sequencing Techniques

Di-deoxynucleotide Chain Termination

Joseph E. Conley, Alex J. Meisel, and James J

DNA Sequencing.

Genetic Research and Biotechnology

Dideoxy chain termination method and the human genome project

DNA sequencing technology

AMPLIFYING AND ANALYZING DNA.

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

DNA sequencing Direct determination of nucleotide sequence

DNA Sequence Determination (Sanger)

DNA strand synthesis by formation of phosphodiester bonds.

Polymerase Chain Reaction (PCR)

DNA Sequencing The DNA from the genome is chopped into bits- whole chromosomes are too large to deal with, so the DNA is broken into manageably-sized overlapping.

Relationship between Genotype and Phenotype

DNA Sequencing A population of DNA fragments is generated.

DNA Sequencing A population of DNA fragments is generated.

DNA Sequencing A population of DNA fragments is generated.

Changing the Living World & Manipulating DNA

5. DNA Sequencing pp

Molecular Biology lecture -Putnoky

Sequencing DNA – the Sanger Method – the most-popular way (one of many methods) A primer binds to one of the DNA strands at a specific location (such as.

Polymerase Chain Reaction (PCR) & DNA SEQUENCING

Plant Biotechnology Lecture 2

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

Relationship between Genotype and Phenotype

Polymerase Chain Reaction (PCR) & DNA SEQUENCING

Presentation transcript:

DNA Sequencing A population of DNA fragments is generated. –One end is common to all fragments (the 5’ end of the sequencing primer). –The other end terminates at all possible positions (the 3- terminus).

2',3'-Dideoxyribonucleoside Triphosphates

Sanger Sequencing

Automated DNA Sequencing Fluorescent dyes are used for detection of DNA chains instead of radioactive isotopes. Products of all four chain terminator reactions are separated through a single gel or capillary tube. Photocells detect fluorescence of the dyes as they pass through the gel or capillary tube. Output of the photocell is directly transferred to a computer for analysis.

Next Gen

Linker scanning the old fashioned way

Two Hybrid

One-Hybrid