In the name of God Summer School Influenza Unit, Pasteur Institute of Iran 1.

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Presentation transcript:

In the name of God Summer School Influenza Unit, Pasteur Institute of Iran 1

Practical approach in virology; Virus quantification method A. Abdoli Sh. Gholami P. Biglari H. Sohani H. Rahmatollahi M. Abdoli Influenza Unit, Pasteur Institute of Iran Summer School

Outline of the lecture 2 fold dilution 10 fold dilution Multiplicity of infection(moi) Hemagglination assay (HA) hemagglutination inhibition assay (HI) Cell culture infective dose %50 (CCID50) Plaque Assay fluorescent-focus assay Influenza Unit, Pasteur Institute of Iran summer

Hemagglutination assay Influenza Unit, Pasteur Institute of Iran summer

5

TCID50 6

Plaque Assay 7

8

cytopathic effect of virus in pfu 9

10

Principal of fluorescent assay 11

fluorescent-focus assay 12

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