Cell Structure III VIBS 443/602 Cytoskeleton and Inclusions.

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Cell Structure III VIBS 443/602 Cytoskeleton and Inclusions

CYTOSKELETON MICROTUBULES (25 nM) MICROFILAMENT (6 nM) INTERMEDIATE FILAMENT (10 nM)

EM 4: liquid droplets are sometimes clustered together in irregularly shaped groups visible in intestinal epithelial cells. Note: the large droplets visible in the goblet cell contain mucin, not lipid. 1. Lipid 2. Chylomicrons 3. Mucus

EM 4 (Enlargement of section from class print): Microvilli at apical ends of intestinal absorptive cells with parallel arrays of microfilaments in their cores. The microvilli are covered with a wispy glycocalyx. The terminal web region immediately below the microvilli contains a very high concentration of microfilaments and (keratin) intermediate filaments, and thereby excludes most other cell organelles. 1. Microvilli 2. Glycocalyx 3. Terminal web

EM 4b: intestinal absorptive cell (apex); 60,000x 1. Microfilaments 2. Microvilli 3. Mitochondria 4. Plasma membranes of adjacent cells 5. Terminal web 6. Zonula occludens

EM 2b: Liver; cytoskeleton elements. Microtubules, microfilaments, and intermediate filaments can be compared in this cell, which has a high concentration of cortical microfilaments. 1. Microtubule 2. Microfilaments 3. Intermediate filaments

EM 19c

EM 6a: Insect spermatocyte. Centriolar region of a cell showing both the stable, triplet microtubule arrays within the centriole, and the labile, individual microtubules originating from pericentiolar material. 1. Centriole 2. Stable microtubule 3. Labile microtubule

EM 6b: monkey oviduct; cross-sectional view of cilia and basal bodies. 1. Basal bodies 2. Cilia 3. Axoneme

EM 6c: monkey oviduct; longitudinal section through basal bodies and cilia. Basal body microtubules serve as assembly sites for outer doublet microtubules that are continuous with them. This is not true for the central pair of microtubules. 1. Basal body 2. Axoneme 3. Cilia

EM 2b

EM 10d: peripheral nerve; Schwann cells and axons. Neurofilaments (intermediate filaments) and microtubules can be seen in both myelineated and unmyelineated axons. 1 Neurofilaments 2 Microtubule

EM 10c

CELL COMPOSITION THREE MAJOR CLASSES OF CYTOPLASMIC STRUCTURE MEMBRANOUS ORGANELLES 2. NON-MEMBRANOUS ORGANELLES – CYTOSKELETAL COMPONENTS etc 3. INCLUSIONS - EXPENDABLES

INCLUSIONS - EXPENDABLES a. NUTRIENT e.g., GLYCOGEN, LIPID b. PIGMENT e.g., MELANIN GRANULES c. SECRETORY GRANULE e.g., ZYMOGEN GRANULE OF PANCREAS

EM 2: liver. Glycogen appears as darkly staining, granular structures that are slightly larger than a ribosome. 1. Glycogen 2. Ribosome 3. Golgi vesicle 4. Lipid

EM 25: ovary. Yolk protein has formed a crystalloid inclusion within the cytoplasm of this oocyte. 1. Crystalloid inclusion

EM 24: corpus luteum. Several clear, non-membrane bounded lipid droplets are visible in the cytoplasm of the large cell. 1. Lipid droplets 2. Mitochondria 3. Smooth endoplasmic reticulum 4. Rough endoplasmic reticulum

Slide 156: MonkeyPancreas; toulidine blue 1. Islet 2. Pancreatic acinar cells

Pancreas, monkey (toluidine blue) 156

EM 1: pancreas. Secretory droplets containing digestive enzymes are major constituents of pancreatic acinar cells. 1. Zymogen granules 2. Rough endoplasmic reticulum 3. lumen

EM 4 Secretory droplets

EM 14: stomach. Secretory droplets with a variety of sizes and staining intensities are visible. 1. Secretory granules 2. Secretory vacuoles 3. Rough endoplasmic reticulum

EM 4c: Intestinal absorptive cell (super nuclear region); 60,000x. 1. Budding RER 2. Coated vesicle 3. Golgi 4. Mitochondria 5. Nucleus 6. Plasma membrane 7. Primary lysosome

EM 20: testis. Several darkly staining lipofuscin granules representing indigestible material are visible in this steroid-secreting cell. 1. Lipofuscin granule 2. Nucleus 3. Mitochondrion

Lung

432432lung with bronchi

432432lung with bronchi

EM 8h: Macrophage in testis; 30,000x. Enlarged basal lamina Heterophagic vacuole Leydig cell cytoplasm Nucleolus Myoid cell

EM 19c Lipid droplets

EM 21: Ductus deferens; 11,000x. 1. Golgi 2. Stereocilia 3. Mitochondria 4. Swirl of SER

Opened Interclated disc in cardiac cells of Heart, endocardium 226

Lipofuscin in cardiac cells of Heart 226

Peripheral ganglion, monkey 492

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