Microbiology: why should we care?. There are 10 times more bacteria in your digestive system than there are cells in your entire body.

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Presentation transcript:

Microbiology: why should we care?

There are 10 times more bacteria in your digestive system than there are cells in your entire body.

There are more microbes on your hand than there are people on the planet

Microbes generate at least half the oxygen we breathe

If a single bacterium with a generation time of 20 min reproduced for 48 hr, the population it produced would weigh about 4000 times the weight of the Earth

Microbes are significant.

Virus

Archaea

VirusArchaea Bacteria

VirusArchaea Bacteria Protists

VirusArchaea Bacteria Protists Characteristics?

Microscopes Probably invented early 1600’s Popularized by Antonie van Leeuwenhoek “Father of microscopy” Discovered microorganisms 1676

Microscopes: light vs. electron

Light microscopy (and Fluorescence microscopy)

Microscopes: light vs. electron Light microscopy (and Fluorescence microscopy) Can be used on living samples Active processes Observe in real time

Microscopes: light vs. electron Light microscopy (and Fluorescence microscopy) Can be used on living samples Active processes Observe in real time Colour

Microscopes: light vs. electron Light microscopy (and Fluorescence microscopy) Can be used on living samples Active processes Observe in real time Colour Maximum magnification: 1000x

Microscopes: light vs. electron Light microscopy (and Fluorescence microscopy) Can be used on living samples Active processes Observe in real time Colour Maximum magnification: 1000x

Microscopes: light vs. electron Electron microscope

Microscopes: light vs. electron Electron microscope 2 million x magnification

Microscopes: light vs. electron Electron microscope 2 million x magnification Black and white

Microscopes: light vs. electron Electron microscope 2 million x magnification Black and white Sample must be dead

Microscopes: light vs. electron Electron microscope 2 million x magnification Black and white Sample must be dead Two-types: Transmission electron microscope (TEM) Extremely thin section of specimen 2-D image

Microscopes: light vs. electron Electron microscope 2 million x magnification Black and white Sample must be dead Two-types: Transmission electron microscope (TEM) Extremely thing section of specimen 2-D image Scanning electron microscope (SEM) 3-D surface image

Microscopes: light vs. electron Electron microscope 2 million x magnification Black and white Sample must be dead Two-types: Transmission electron microscope (TEM) Extremely thing section of specimen 2-D image Scanning electron microscope (SEM) 3-D surface image

Electron microscope 2 million x magnification Black and white Sample must be dead Two-types: Transmission electron microscope (TEM) Extremely thing section of specimen 2-D image Scanning electron microscope (SEM) 3-D surface image Microscopes: light vs. electron

Important parts:

Calculations: magnification

3.2 cm

Calculations: object size

Microscope lab skills Safe behaviour! Carrying your microscope Creating a slide Focusing your microscope: 4x, 10x, 40x Troubleshooting