DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

Slides:

Advertisements

Similar presentations

Biotechniques. Gel electrophoresis Separates molecules according to size and charge. Different sized segments of DNA cut by restriction enzymes Segments.

Advertisements

Gel Electrophoresis.

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

Separation of Proteins by Gel Electrophoresis Carolina Biological Kit #

“Gel electrophoresis”. Gel electrophoresis is a procedure for separating a mixture of molecules through a stationary material (gel) in an electrical field.

LAB SIX Molecular Biology. Three samples of phage lambda DNA: 1.Digested with EcoRI 2.Digested with HindIII (fragments are of known size) 3.Undigested.

1,000,000 1,000,000,000 PCR cycles Theorectical yield Actual yield “Plateau effect” No. of DNA copies How much DNA amplification has occurred?

& Gel Plasmid Electrophoresis Mapping.

Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Gel Electrophoresis Biotech I.

Gel electrophoresis Separating molecules by size and charge.

Electrophoresis Electrophoresis is the movement of molecules by an electric current .This is practically done in a matrix to limit migration and contain.

Connect!  What is physical therapy?  What is respiratory therapy?  What does “therapy” mean?  So, what do you think “gene therapy” might mean?

Aim: How do scientists identify people using DNA Fingerprinting?

DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!

Unit 8 Vocabulary 1. micropipette- tool used to transfer small volumes of liquid 2. P 20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used.

Biotechnology. Southern Blot/Electrophoresis Electrophoresis- separate DNA with electricity Step 1: DNA is isolated and cut with restriction enzymes –Makes.

Biotechnology Introduction Gel Electrophoresis. Method of analyzing DNA Allows a researcher to determine how alike or different two samples of DNA are.

Visualizing DNA. What is it?  Gel electrophoresis is one of the techniques scientists use to look at the DNA they have.  This technique separates DNA.

 DNA can be collected from any living tissue:  blood, skin, hair, urine, semen, sweat or tears.

Agarose (Horizontal) Gel Electrophoresis Malasian word for seaweed is “agar-agar”. Agarose is derived from red seaweed. Electrophoresis means “carrying.

PCR is DNA replication in a test tube Ex. 25: PCR Based Testing for Water Contaminants Day 2.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

(RFLP Electrophoresis)

Gel Electrophoresis. Definition – COPY ME! Separation of DNA fragments according to size and charge Based on movement through a gel medium when an.

Gel Electrophoresis.  This workforce solution was funded by a grant awarded under the President’s High Growth Job Training Initiative as implemented.

Gel Electrophoresis of DNA. DNA as Forensic Evidence Individual evidence – identify a single person Trace evidence – small amount left at crime scene.

DNA fingerprinting is not taking someone’s fingerprint. It is cutting up a DNA strand and separating them by size.

Gel Electrophoresis.

DNA Profiling (DNA fingerprinting) pard/cleared.html.

Semester 2 Final Review Part 1 Genetics, Biotechnology, Protein Synthesis and Evolution.

Gel Electrophoresis of DNA. What is Gel Electrophoresis? Electro = flow of electricity, phoresis, from the Greek = to carry across A gel is a colloid,

Page Gel Electrophoresis gel electrophoresis – moving DNA through a gel medium using an electric current Why can we move DNA with electricity?

Gel Electrophoresis What is Gel Electrophoresis? Gel electrophoresis separates molecules on the basis of their charge and size. The charged macromolecules.

What happens now that the DNA has been extracted?

Gel Electrophoresis By Erin Martin & Satya Moolani.

DNA Fingerprinting Techniques

Analyzing DNA Fragments AP Biology Fall DNA Fingerprint  DNA fingerprint: unique array of base sequences in each organism that is slightly different.

Estimation of Nucleic Acid NAHLA BAKHAMIS. 1.Agarose Gel Electrophoresis: Separation and analysing DNA of varying sizes, by moving –ve charge na through.

Biotechnology. Gel Electrophoresis A technique that separates macromolecules on the basis of their rate of movement through a gel under the influence.

One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects and compare it.

1 DNA Technology Part I: Restriction Enzymes & Electrophoresis Created by: C. Massengale Edited by: Beth Walker.

Gel Electrophoresis. What is Gel Electrophoresis? Electrophoresis: Movement of charged particles through a solution (gel) under the influence of an electric.

Gel Electrophoresis.

How do scientists identify people using DNA Fingerprinting?

Lecture 4 January 7, 2016 Biotech 3.

DNA Paternity Test RFLP analysis

DNA Fingerprinting One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects.

By Zainab sajjad (117114) Ayesha Rehman (117115)

Gel Electrophoresis of DNA

Gel Electrophoresis By: Sariah Arnold.

DNA Fingerprinting.

Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments.

Restriction Enzymes & Electrophoresis

DNA ELECTROPHORESIS OR DNA FINGERPRINTING.

Technology that uses electricity to separate molecules in a gel slab

Electrophoresis.

Visualizing DNA Gel Electrophoresis.

DNA FINGERPRINTING Gel Electrophoresis

DNA Fingerprinting and Gel Electrophoresis Notes

Chapter 7 DNA Fingerprinting.

Restriction Enzymes The ability to cut and paste DNA predictably is due to the use of restriction enzymes. They were first identified in and isolated.

Creating a DNA Fingerprint by Gel Electrophoresis

Introduction to Gel Electrophoresis

Gel Electrophoresis Ms. Cuthrell.

Separating molecules by size and charge

How to fingerprint the bad guy

Presentation transcript:

DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

What is Gel Electrophoresis? Process used to separate fragments of DNA using a charge

1 st Step in Electrophoresis Prepare the gel Need agarose, mold, and comb

2 nd Step in Electrophoresis Prepare the box Need electrophoresis chamber and buffer

3 rd Step in Electrophoresis Load DNA into the wells of the gel using a micropipette DNA Well

4 th Step in Electrophoresis Run electricity to separate DNA fragments

5 th Step in Electrophoresis Stain the gel to see the DNA fragments Analyze results

What is the Result? Well DNA Fragment Shorter Fragments Larger Fragments ___ +

How to read a gel? Start by reviewing paternity bands (from Mom and Dad) Larger Smaller Sex D1 = Real Daughter D2 = Daughter of Dad S1 = Real Son S2 = Adopted Son

EXAMPLE I: WHO IS THE CRIMINAL?

EXAMPLE II: WHO IS THE CRIMINAL?

Example III: WHO IS THE CRIMINAL?