Presented By Junaid Gabol

Slides:

Advertisements

Similar presentations

Lab 6. Gram staining was originally developed in 1844 by Christian Gram Because most bacteria have one of these two types of cell walls, we can use this.

Advertisements

Watershed Assessment Analysis of Data. Q Values Raw data values are NOT Q values. We need to convert the raw data/observations into “grades” (Q values)

381 PHT Lab #2.

Hazards to Human through Food Chain Bacterial Viral Toxins Drug Residues.

Identifying and Controlling Microbes Unit 7 Donna Howell Medical Microbiology Blacksburg High School.

General Microbiology Laboratory By: Mahmoud W El-Hindi1.

Protecting people and livestock through pathogen treatment Microbiology Environmental microbiology water, wastewater, soil, air Medical microbiology, pathology,

Food Microbiology Risk Reduction Program for Rutgers Dining Halls.

Observation of bacteria using staining procedures Simple staining Gram staining.

By: Prof. Eqrar. UseTypical quality parameters Public Water SupplyTurbidity, TDS, inorganic and organic compounds, microbes Water contact recreationTurbidity,

CULTURE MEDIA LECTURE 5: Microbiology and Virology; 3 Credit hours

Differential Stains (Gram stain & Acid Fast Stain) Abdelraheem BA

Review of Gram Stain Selective and differential Media

Wastewater Treatment Plants & Bacteria Strategies for Compliance Best Practices for Effluent Sampling TANNY BUSBY & LAURA BONJONIA ENVIRODYNE LABORATORIES,

Food Safety & Sanitation Foods & Nutrition 1 Food Borne Illness Result from eating contaminated foods For bacteria growth warmth, moisture, and food.

Microbiology Overview KWWOA April 09, 2014 Department for Environmental Protection Energy & Environment Cabinet.

Classic Culture Procedures MAJ (First Mi. Last) Chief, Food Analysis Department.

Interplay between food safety climate, food safety management system and microbiological hygiene and safety ir. Elien De Boeck Prof.dr.ir.

Lab 4 Goals and Objectives: ***Use wax pencils (China Marker): Sharpie will wash off with alcohol! Be sure to resuspend broth cultures completely! Exercise.

QUALITY CONTROL IN MEDICAL MICROBIOLOGY Quality control is divided Internal quality control: performed locally inside External quality control:

Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Umass Food Science EXTENSION RESEARCH

Turn Key Suppliers & Consultants For Samarthan Systems Pvt Ltd..

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Class Four Microbiology Quiz  Quiz Number One – Open Response and Identification  Microscope  Aseptic techniques  Culture transfer techniques.

Gram staining. Types of staining Simple –Bacteria stained to contrast environment Differential –Bacteria differing chemically react differently to staining.

Born from the Governor’s efforts to engage all stakeholders to solve problems Designed to provide technical support to local organizations ODEQ Program.

Experiment 6 Isolation & identification of pathogenic cocci

Lab 4 Lab 3 Goals and Objectives

PREPARATION OF A SMEAR GRAM STAIN ACID FAST STAIN

Lab # 3 Gram and Acid Fast stain Medgar Evers College Biology 261 Prof. Santos.

 Prepare a separate smears of E. coli and S. aureus on two different slides. Fix these preparation by allowing them to dry by passing over the Bunsen.

Safety Notes Bunsen burners> open flame

Biology 11 Microbiology Gram staining. What is Gram Staining? Basic classification of bacteria that is based on the cell wall structure. There are 2 main.

TOPICS Staining and stain Staining and stain Types of staining techniques Types of staining techniques Gram Staining - Principles Gram Staining - Principles.

THE GRAM STAIN. The gram stain is the most widely used staining procedure in bacteriology It is called a differential stain it differentiates between.

GRAM STAINING. OBJECTIVES ● Describe reagents used in Gram stain & purpose of these reagents. ● Color expected of Gram Pos & Gram Neg after performing.

Good Morning! Please pick up the yellow Gram- Staining Lab worksheets from the overhead cart. Grab some goggles and a lab apron (in box underneath goggles).

Preparing Slides There are 4 different ways of preparing slides – the one you pick is dependent on the specimen and resolution required. The four types.

BTEC 223 Lab Exercise Water Module

Food Safety & Sanitation

GRAM and ACID FAST STAINS

PRACTICAL GRAM STAINING

Food Safety & Sanitation

بسم الله الرحمن الرحيم 140 Micro Lab 7: Gram stain.

Increase in number of cells, not cell size Populations Colonies

Lecturer Ishraq Hasan Elewi.

Gram Staining: Principle, Procedure and Results MSc. Sarah Ahmed

Bacterial Cell Walls.

PRACTICAL GRAM STAINING

THE GRAM STAIN.

2 loops each organism Wax Pencil mixed together

Faculty of Medicine and Health Sciences

Laboratory facilities

PRACTICAL GRAM STAINING

الفحص البكتيريولوجى لمياة الشرب والصرف الصحى

Untouched purity from a Norwegian artesian well to the consumer

General Microbiology Laboratory

CHAPTER 10!!!!!yeAAAAAAAAA Cleaning & Sanitizing

Food Safety FACS 7 Mrs. Otos.

Food Safety & Sanitation

Classification by Structure

Medical Microbiology Lab. 3 By Assistant lecturer Zainab farooq shafeeq.

Presentation transcript:

Presented By Junaid Gabol alshaheer corporation (pvt) Ltd Microbiological, Chemical Laboratory Testing and Ro Water Quality Assurance Presented By Junaid Gabol

Salmonella (kit method) Total plate count (TPC) (Dilution Method) Meat tests E.Coli 0157 (kit method) Salmonella (kit method) Total plate count (TPC) (Dilution Method) Staph.aureus (Dilution Method) Coli forms (MPN Method) (Total coli forms, Fecal coli forms)

Slides preparation (Difference b/w gram +ve and gram _-ve bacteria 1. Smear preparation 2. Crystal violet 3. Iodine solution 4. Alcohol 5. Saffranine 6. Microscopy (Gram+ve will be appear purple and Gram -ve will be appear pink colour)

(Plate Exposure Method) Area Monitoring Tests Beef Hall Mutton Hall Debone Hall Chillers Trucks Ro plant Chemical Lab Micro Lab (Plate Exposure Method)

Swab tests 1.Beef Hall 2.Mutton Hall 3.Micro Lab 4.Meat contacts (knocking box, Hooks, conveyors, trolleys, tunnels, saws, knives) 2.Mutton Hall (slaughtering table, hooks, conveyors, trolleys, tunnels, knives) 3.Micro Lab (knives, cutting board, Meat grinder jar, laminar cabinet, Equipments) 4.Meat contacts (fabric, tags, chillers walls, chillers hooks, weight table, trucks)

Hands dabs and hands swabs 1. Workers hand dabs (House keeping staff) 2. Butchers hand dabs (Mutton and beef butchers) 3.Labor hand dabs (loading and carcass handling staff) 4. Q/A and lab staff hand dabs

Total plate count Pseudomonas (Filtration method) (Dilution method) Water Tests Pseudomonas (Filtration method) Total plate count (Dilution method) 3. Coli forms (Total coli forms, Fecal coli forms) (MPN method) (Ro water, bore water, drinking water, animals drinking water)

Chemical Tests Hardness of water (hardness kit) 2. TDS (Total dissolved solids) (calibration of tds meter) 3. pH of water (calibration of ph meter) 4. Chlorine estimation (chlorine test kit)

1. Temperature of carcasses 2. Temperature of chillers Physical Monitoring 1. Temperature of carcasses 2. Temperature of chillers 3. PH of Meat (All parameters of compliances

Reverse Osmosis Plant For Water PROCESS FLOW OF RO WATER 1. Sand filters 2. Micron filters 3. Ro membranes 4. Uv light Product water for washing and chemical testing (Tds, Ph, hardness)

Thank you