FPIA results in an inverse dose response curve such that lower levels of patient analyte result in a higher signal (in this case, the signal.

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Presentation transcript:

FPIA results in an inverse dose response curve such that lower levels of patient analyte result in a higher signal (in this case, the signal is polarized light). High signal at low patient analyte levels results in a highly sensitive Assay.

If high concentrations of the sample analyte are present more specimen analyte binds to the antibody, leaving the analyte tracer unbound. If low concentrations of the sample analytes are present less specimen analyte binds to the antibody leaving analyte tracer bound.

Polarized light can be used to produce a polarized flurorescent emission from the analyte-tracer. The average polarization of the emitted fluorescence is related to the speed of rotation of the molecule. The rate of molecule rotation in liquid is related to the size of the molecule. Small, unbound analytes rotate more rapidly than the larger analyte-antibody complex. Axsym system undergo to this priniciple.

Measurement of plasma prolactin has been used as an index of response to the injection of TRH, which stimulates release of prolactin in addition to stimulating the release of TSH.(clinical notes of clinical chemistry). If you are taking medicine for a prolactinoma, you will have your hormone levels checked at least once or twice a year.

When performing the assay slowly bring samples to room temperature. It is recommended that all samples be assayed in duplicate. DO NOT USE HEAT-TREATED SPECIMENS. This test cannot be made for mother at lactation period. Patient should be avoid Emotional stress and strenuous exercise.