Living up to your Nernst potential: a graphical approach to electrochemical gradients Gregory J. Crowther, Ph.D. –∞ +∞ Membrane potential Net driving force OUT IN Tues., Feb. 9 2:30pm Discovery 464

Learn about coral microbiomes from Jesse Zaneveld Student lunch : Thursday, February 11 th at 12:00 pm. for Research Talk : Thursday, February 11 th at 2:00 pm in UW2-307 Title: Exploring the Microbial Wilderness: Theory, Tools, and Applications from Chronic Disease to Wildlife Conservation Teaching demonstration : Friday, February 12 th at 11:00am in UWBB 205 Title: Interactive Demo: Key Perspectives on Microbial Communities in Health and Disease Photo from:

David Sommerfeld, Ph.D.

BBio 351 – February 1, 2016 Today’s outline Finish endocrinology (adrenal disorders, thyroid paper) Start Chapter 16: Reproduction! More endocrinology, really Return of the hypothalamic-pituitary axis H. Hasuwa et al., Science 2013 Examples of / exceptions to negative feedback Ovarian/uterine cycle LH surge Birth control

Reproduction, homeostasis, negative feedback

Reproduction: connection with endocrinology F. H. Martini et al. (2014)

Gamete and embryo development Sherwood Figures 16-3 and 16-4c

Female (cat) reproductive anatomy biology.clc.uc.edu/fankhauser/

Male reproductive anatomy CAT biology.clc.uc.edu/fankhauser/ SHEEP fau.pearlashes.com/anatomy/ dissections/reproductive/testis10.jpg

The ovarian & uterine cycles 10 th Martini Figure (like Sherwood Figure 16-16) Day 0 Day 14 Day 28

The ovarian & uterine cycles 10 th Martini Figure Day 0 Day 14 Day 28

10 th Martini Figure (like Sherwood Figure 16-17)

What’s “wrong” with this picture? Sherwood Figure 16-18a

Tracking the cycle to promote or avoid pregnancy Urine test – which hormone should be tracked?

Birth control: “the pill” What does it contain? How is pregnancy avoided? Prevent ovulation? Prevent fertilization? Prevent implantation? Picture: JournalTimes.com

faculty.washington.edu/crowther/Misc/Songs/

New research on the hypothalamic-pituitary-gonadal axis: H. Hasuwa et al., Science 341: 71-73, 2013 Title: “miR200b and miR429 function in mouse ovulation and are essential for female fertility” Review: hypothalamic-pituitary-gonadal axis Main question: exactly does GnRH promote LH secretion? Review: control of gene expression

What are microRNAs (miRNAs)? Stefanie S. Jeffrey, Nature Biotechnology 2008

Genetic tools/tricks: reporter assay to see how miR-200b & miR-429 affect Zeb1 expression Hasuwa et al. (2013), Figure S13

Genetic tools/tricks: overexpressing Zeb Google Images search results; Hasuwa et al. (2013), Figure 3B-C

Literature-reading tip: figure (and table) titles often collectively tell the story! Fig. 1. Infertility phenotype of miR-DKO mice. Fig. 2. Pituitary defects in miR-DKO female mice. Fig. 3. Lhb expression is regulated by ZEB1 as a potential target of miR-200b and miR-429.

Fig. 1. Infertility phenotype of miR-DKO mice. Hasuwa et al. (2013), Figure 1A

Fig. 1. Infertility phenotype of miR-DKO mice. Hasuwa et al. (2013), Figure 1C-F

Fig. 2. Pituitary defects in miR-DKO mice. Hasuwa et al. (2013), Figure 2A-D

Fig. 2. Pituitary defects in miR-DKO mice. Hasuwa et al. (2013), Figure 2E-H

Fig. 3. Lhb expression is regulated by ZEB1 as a potential target of miR-200b and miR-429. Hasuwa et al. (2013), Figure 3D-E

Diagram to summarize main findings?

What questions could be researched next?