NON-INVASIVE MEASUREMENT METHOD OF SKIN CONDITIONS USING DERMALAB® COMBO Presented by: Asst. Prof. Dr. Hazrina Ab Hadi BPharm, Hons., MSc. Drug Delivery, PhD Pharmaceutics Department of Pharmaceutical Technology Kulliyyah of Pharmacy International Islamic University Malaysia

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

Skin structure and function Schematic illustration of cross-section of human skin (adapted from Servier Medical Art)

DermaLab® Combo

TEWL open chamber probe Principle of vapor diffusion gradient Sensor inside - to avoid any air currents and direct light. The TEWL measurement is expressed as g/m 2 /hr.

Hydration pin probe Conductance principle is applied Measure skin moisture. Conductivity - to measure the water binding capacity of the stratum corneum.

Skin color probe Based on the light absorption by the skin The color measurement is based on the principle of reflectance spectrophotometry. This probe has an optics design in order to ensure minimal sensitivity to ambient light condition.

Elasticity suction probe Measure the necessary force needed to lift the skin Provide a light weight probe Skin elasticity of subjects is determined by refraction time (R) once the negative pressure is released.

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

Aims and Objectives To evaluate biophysical parameters (TEWL, hydration, melanin content and elasticity) of the skin using Dermalab Combo To compare skin parameters between genders To identify possible factors that affect functional state of the skin based on the skin condition measurements

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

Methodology 100 volunteers (IIUM students) Age: 18 – 27 Consent Form + simple questionnaire (demographic profile and daily activities) Acclimatize to ambient T Measurement on the volar forearm

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

Results and Discussion 1/9 TEWL in different gender

Results and Discussion 2/9 Use of moisturiser vs TEWL

Results and Discussion 3/9 Average hydration between genders

Results and Discussion 4/9

Results and Discussion 5/9 Average melanin content between genders

Results and Discussion 6/9 Type of activity vs Melanin content

Results and Discussion 7/9 Sunblock usage vs Melanin content

Results and Discussion 8/9: Skin Elasticity between genders

Results and Discussion 9/9: Use of moisturiser vs elasticity Not Used

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

Conclusions Biophysical parameters of the skin vary among different genders. The differences may be due to individuals daily activity, use of skin care products as well as environmental factors. The use of DermaLab® Combo in this experiment provides a reliable measurements for TEWL, hydration, pigmentation and elasticity. Thus, this baseline reading can be used for further research study such as comparison of skin care products and assessment of skin care efficacy.

OUTLINE Background Aims and Objectives Methodology Results and Discussion Conclusions References

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