Thomas Crouzier, 2015 Engineering mucins for biomedical applications Thomas Crouzier biopolymersforlife.org WBC 19 th of May 2016 Page 1.

Slides:

Advertisements

Similar presentations

Advanced Techniques for Pore Structure Characterization of Biomedical Materials Akshaya Jena and Krishna Gupta Porous Materials, Inc. 20 Dutch Mill Road.

Advertisements

Gastric Mucosal Barrier

3.2 Review PBS.

Hyaluronic Acid Hydrogel Films October 27, 2008 Scott Zawko – Post-doctoral Researcher (Presentation to Dr Raymond Grill)

Effective loading and controlled release of camptothecin by O- carboxymethylchitosan aggregates Liu Nan 4 th semester in Ph. D course Lab. of Biopolymer.

Micron-sized particles Li Junhua Abstract Micron-sized agarose particles were prepared using emulsification/ gelation method as a reservoir.

Nanocellulose in a given medical application

Gel Electrophoresis.

Stimuli Responsive Polymers

History of Cell Theory Why Cells? Limits of Light Eye Piece MagObjective Mag 10x4x 10x 40x 100x (oil emmersion)

Agarose Gel Electrophoresis 1Dr. Nikhat Siddiqi. Agarose is a linear polymer made up of the basic repeating unit of agarobiose which comprises alternating.

Richelle C. Thomas, Christine E. Schmidt University of Texas at Austin.

Photocured Films of Hyaluronic Acid Functionalized with Cyclodextrin

Richelle C. Thomas, Christine E. Schmidt Department of Chemical Engineering University of Texas at Austin 1.

Nanoparticles and their medical applications

CELL COMPOUNDS Water Acids, Bases & Buffers Water Acids, Bases & Buffers.

Laboratory Activity Nine

Projects in the research area Hybrid bionanosystems based on self-assembled structures, quantum dots, plasmonic and magnetic nanoparticles Strategic Line.

CHAPTER 2 The Nature of Molecules. Which Elements Are Most Significant To Biologists? Six of these plus a few other trace elements.

Chapter 4 Introduction to Nanochemistry. 2 Chapter 4 Periodicity of the Elements Chemical Bonding Intermolecular Forces Nanoscale Structures Practical.

NanoPackSafer – um novo sistema de embalagem de alimentos António A. Vicente Departamento de Engenharia Biológica Universidade do.

Introduction and History Self-Assembling Carbohydrates and their Application in the Fight Against Cancer By: Kelly Hunt University of New Hampshire, Department.

Figure 5: (a) Confocal section of m-PEG particle distribution (green) in a tumor spheroid (nuclei stained blue) (40x magnification) (b) Confocal section.

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. CHAPTER 2 LECTURE SLIDES.

Introduction to cell–hydrogel mechanosensing by Mark Ahearne Interface Focus Volume 4(2): April 6, 2014 ©2014 by The Royal Society.

NANOPARTICLES FOR DRUG DELIVERY: THE SMALLER, THE BETTER ? Gurny R., Pourtier M., Vargas A., Delie F. Department of Pharmaceutics and Biopharmaceutics.

Protein Transduction David Shiuan Department of Life Science Institute of Biotechnology National Dong Hwa University.

Liposomes: Formation, preparation, properties and applications

Bioselective Surfaces Harvey Hoch, Convener, Cornell Pengju Luo (Clemson) Jeremy Tzeng (Clemson) Paul Millard (U of Maine) Bruce McPheron (Penn St.) Bruce.

IB2.5.3 Animal tissues © Oxford University Press 2011 Animal tissues.

E-beam Size-Dependent Self- Assembly Protein Array.

UNFIGURE FIGURE 1.1. Dimensions of some example cells. 1 mm = 10 −3 m; 1 µm = 10 −6 m; 1 nm = 10 −9 m.

DNA Fingerprinting 1.How do we do it ? 2. What do we use it for?

Copyright © 2010 Pearson Education, Inc. An Introduction to Cells Cell Theory –Cells are the building blocks of all plants and animals –All cells come.

CELL MEMBRANE STRUCTURE AND FUNCTIONS By. Dr Nazish.

Applications & Analysis DNA Gel Electrophoresis 1.

ISOLATION, SEPARATION AND DETECTION OF PROTEINS part I Michael Jelínek, Jan Šrámek Lenka Rossmeislová.

Biological Molecules B2, B3, B4. Water and It’s role in biological Systems  describe how the polarity of the water molecule results in hydrogen bonding.

Dr. Mustafa Saad Histology - Introduction 1. Histology is the study of the various tissues of the body; how these tissues appear, how they interact with.

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property Shuai Wu a,d, ZhuoyaoHuang a, JianhuiYue a,d, DiLiu a,c,

MUHAMMAD ALAM Supervisory Team: Dr Amal Ali Elkordy & Dr Timothy paget Department of PHW, University of Sunderland. UK TITLE: Local mucoadhesive drug delivery.

Porous-Wall Hollow Glass Microsphere as novel potential nanocarriers for biomedical applications Shuyi Li, M.D., Ph.D. NIH Nanomedicine Center for Nucleoprotein.

PEG Hydrogel Coating for Medical Devices PEG Hydrogel Coating for Medical Devices B. Mulawka, B. Roedl, P. Schenk, D. Patel Advisor: Professor William.

Chapter 5 A Journey into the Eukaryotic Cell Mrs. Park December 2015.

Advanced Drug Delivery Reviews 54 (2002) 3–12 Hydrogels for biomedical applications Allan S. Hoffman.

Co-cross-linked chitosan hydrogel as carrier for the local delivery of cisplatin. Liposome inclusion. Maria José Moura 1,2, Maria Helena Gil 2, Maria Margarida.

Histology - Introduction

Date of download: 10/1/2017 Copyright © ASME. All rights reserved.

Composite Polysaccharide Hydrogels

Calculating Diffusion Coefficients of Dyes in Hydrogels

The Release Of Curcumin From Alginate Beads In Different Media

Fig. 1. Optical microscopy images of protoplasts and vacuoles isolated from C. roseus leaves. Protoplast fraction (A), vacuole fraction (B), and Western.

Tailoring drug release kinetics from peptide hydrogels Mohamed A

MICROSCOPY AND STAINING

Maryam Rahmati 1, Ali Samadikuchaksaraei 2,3,4, Masoud Mozafari 1,5,

No measureable binding

Designs of adipocyte-specific binding microgel for fat reduction

1.3 - Cell Membrane Structure

Low-Molecular-Weight Gels: The State of the Art

Cell wall structure. Cell wall structure. (A) Transmission electron micrograph of a section of a C. albicans cell prepared by freeze-substitution, showing.

FACT neither disassembles nor binds properly assembled nucleosomes.

Volume 113, Issue 9, Pages (November 2017)

Comparison of Matrigel and type-I-collagen matrix architecture.

Fig. 2 Mineralization and surface colonization of films of three PBAT [poly(butylene adipate-co-terephthalate)] variants during a 6-week soil incubation.

Electron micrograph of negatively stained protofilaments assembled in vitro from E. coli FtsZ (1 μM FtsZ, 50 mM MES [morpholineethanesulfonic acid] [pH.

Thermosensitive chitosan hydrogels for biomaterial applications

Fig. 3 Characterization of PCNs and PCN-loaded microneedles.

Fig. 1 Photoluminescence characteristics of the exciplex system.

SEM and TEM images and photoluminescence properties of composites

Presentation transcript:

Thomas Crouzier, 2015 Engineering mucins for biomedical applications Thomas Crouzier biopolymersforlife.org WBC 19 th of May 2016 Page 1

Thomas Crouzier, 2015 Page 2 Research on extracellular matrix Biomedical products Cosmetics Hyaluronic acid

Thomas Crouzier, 2015 Page 3 Collage n Research on extracellular matrix Biomedical products Cosmetics

Thomas Crouzier, 2015 Page 4 Chitosan and Chitin Agriculture Biomedical products Cosmetics

Thomas Crouzier, 2015 Mucins Page 5

Thomas Crouzier, 2015 Author unknown Page 6 Mucins

Thomas Crouzier, 2015 Page 7 From our infographic “Mucus, it’s snot what you think”:

Thomas Crouzier, 2015 Mucins cover our wet epithelium Page 8

Thomas Crouzier, 2015 Tomas Sandberg, Hans Blom, Karin D. Caldwell. J Biomed Mater Res A Dec;91(3): Mucins’ chemical structure Page 9

Thomas Crouzier, 2015 Mucins’ interesting properties Hydration Lubrication Selective filter Physical gels Intermolecular interactions Cell repulsion Page 10

Thomas Crouzier, 2015 In house from pig stomachs Commercially available mucin (Sigma-Aldrich) Other animal or plant sources: Mucin extraction Page 11

Thomas Crouzier, 2015 Mucin coatings Mucin thin films Mucin hydrogel 12 nm µm mm Page 12 Assembling mucins into materials Crouzier et al. Biomacromolecules 14, 3010–3016 (2013). Co et al. Adv. Mater. Interfaces 2, (2015). Polak et al. Biomacromolecules 15, 3093–3098 (2014). Crouzier et al. Biomacromolecules 13, 3401–3408 (2012). Duffy et al. Acta Biomater. 20, 51–59 (2015).

Thomas Crouzier, D mucin hydrogels for drug delivery Page 13

Thomas Crouzier, 2015 Drug delivery in soft tissue engineering. 2011, Vol. 8(9) p Controlled drug release Page 14

Thomas Crouzier, 2015 Hydrogels for drug delivery Cortisone release from hyaluronic acid hydrogel J. Control. Release, 13 (1990), pp. 33–41 Page 15

Thomas Crouzier, 2015 Mucins are sticky molecules Mucins bind molecules that are hydrophobic, thiol-bearing, positively charged Page 16

Thomas Crouzier, 2015 Methacrylation of mucins Photo-crosslinked with Irgacure UV light Page 17

Thomas Crouzier, 2015 Scanning electron microscopy 5 µm 1 µm 4 mm Page 18

Thomas Crouzier, 2015 Model drugs Paclitaxel (Hydrophobic anti-cancer) Polymyxin B (Positively charged antibiotic ) Page 19

Thomas Crouzier, 2015 Polymyxin B (hydrophilic, positively charged) Sustained release from BSM-MA gels Page 20

Thomas Crouzier, 2015 Hydrophilic positively charged Sustained release from BSM-MA gels Page 21

Thomas Crouzier, 2015 Paclitaxel (hydrophobic) Hydrophilic positively charged Sustained release from BSM-MA gels Page 22

Thomas Crouzier, 2015Page 23 Drug release from BSM-MA gels Lieleg, O. & Ribbeck, K. Biological hydrogels as selective diffusion barriers. Trends Cell Biol. 21, 543–551 (2011). Size filtering Interaction filtering

Thomas Crouzier, 2015 Control molecules Dextrans (Neutral, high MW) Polyglutamic acid (negatively charged, high MW) Page 24

Thomas Crouzier, 2015 Hydrophobic drug Hydrophilic drug Polyglutamic acid (hydrophilic) Sustained release from BSM-MA gels Page 25

Thomas Crouzier, 2015 Sustained release from BSM-MA gels Dextran (5 kDa) Hydrophilic drug (1.3 kDa) PGA (25 kDa) Hydrophobic drug (0.8 kDa) Page 26

Thomas Crouzier, 2015Page 27 Dextran release from BSM-MA gels Lieleg, O. & Ribbeck, K. Biological hydrogels as selective diffusion barriers. Trends Cell Biol. 21, 543–551 (2011). Size filtering Interaction filtering

Thomas Crouzier, 2015 Dextran release from BSM-MA gels Page 28

Thomas Crouzier, 2015 BSM-MA (-) + polymyxin Antimicrobial effect of BSM-MA gels Page 29

Thomas Crouzier, 2015 No gelBSM-MA BSM-MA + Paclitaxel Antimicrobial effect of BSM-MA gels Page 30

Thomas Crouzier, 2015 Katharina Ribbeck Connor Duffy Funding: Thanks to: Page 31 Xueying Zhong Victoria Linderberg Sujit Kootala

Thomas Crouzier, 2015 Thank you for listening! Page 32

Thomas Crouzier, 2015 Measuring drug release Prepare BSM-MA gel with fluorescently labeled drug mixed in Release by incubating with PBS at 37ºC Measure fluorescence of supernatant Thomas Crouzier, 2014 Page 33

Thomas Crouzier, 2015