Recognized in 1976 in USA among attendees of a legion (army) convention It has 9 genera and 36 species L. Pneumophila is.

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Presentation transcript:

Recognized in 1976 in USA among attendees of a legion (army) convention It has 9 genera and 36 species L. Pneumophila is the commonest Gram negative rod, aerobic, flagellated

Structure of L. Outer Inner Blebs Capsule (polysaccharide 8 serogroups are Each serogroup has got specific antigen

Pathogenicity of It possesses: # Endotoxin # Haemolysin # Normal flora of amoebae in Survives in chlorinated Transmission: # By inhaling air conditioning After inhalation, it infects the lungs.

Legionnaires‘ Disease Fever, dry cough, dyspnoea, Pneumonia in lung disease Encephalopathy, peripheral neuropathy. # No person to person spread.

Laboratory Specimens: sputum, urine, Inoculation of guinea pigs, chick embryos, and cell Inoculation of selective Mueller Hinton agar with hemoglobin, vitamins, Incubate in 5% CO 2 Identification: morphology, serology.

Serological Rapid microagglutination Immunofluorescence antibody Antigens detected in urine by PCR to detect specific ribosomal RNA.

L. Pneumophila Ciprofloxacin Cleaning of air-conditioners & Water chlorination every six Water pipes are made of material not supporting growth of Amoebicides to destroy amoebae