Revision G 8/22/13 PARC/T. Cassady Pro K  FD15 (+/- 5 days), FD30 (+/- 14 days), FD60 (+/- 14 days), FD120 (+/- 14 days), and FD180/R-14 (+14/-0 days)

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Revision G 8/22/13 PARC/T. Cassady Pro K  FD15 (+/- 5 days), FD30 (+/- 14 days), FD60 (+/- 14 days), FD120 (+/- 14 days), and FD180/R-14 (+14/-0 days) sessions required per subject.  At least 7 days between FD15 and FD30 sessions; at least 3 weeks between all other sessions; final session within 2 weeks of landing, but at least 3 weeks from the previous session.  Frozen blood collection sessions are combined with concurrent sessions for Biochem Profile and/or Repository when subjects also participate in these experiments. When combined, refer to this slide. Session Day -1 (Day 4) :15 HRF-F/BLD-OPS25_SUB (ProK, ProK/Repo sessions post-sleep) OR HRF-F/BLD-OPS35_SUB (ProK/Biochem/Repo session post-sleep) HRF-RC-SPIN 30 Session Day (Day 5) HRF-F/BLD-SETUP :10 HRF-RC-CNFG HRF-F/BLD-STOWHRF-SPIN-CNCLD HRF-MELFI-INS OR HRF-GLCR-INS < 1:10 HRF2-RACK PWRUP-CMD :05:10 :20 :25 OR :35 HRF2-RC-TRACK :30 ESA-HRF2-ACT (ESA) HRF2-RACK/PC-TRACK :30 mDPCmDPC CREWWAKECREWWAKE HRF-MED TRNR-CONSULT 1:00 ESA-HRF2-DEACT (ESA) :30 :05 HRF2-RACK PWRDN-CMD HRF Generic Frozen Blood Collection Crew Commanding Additional Activities Unattended TEXTFlexible HRF-F/BLD-COAG :15 HRF-SAMPLE-PHOTO (one FD session/subject, any photo camera operator) HRF-F/BLD-OPS_OPR REMINDER-HRF F/BLD (pre-sleep)

Revision G 8/22/13 PARC/T. Cassady Pro K HRF-URINE-OPS_SUB :20 24 hrs :20:05:20 HRF-URINE-STOW HRF-URINE-OPS_SUB (post-sleep) HRF-URINE-OPS_SUB HRF-MELFI-INS OPS OR HRF-GLCR-INS OPS :05:20 :05 HRF-MELFI-R/INS OPS OR HRF-GLCR-R/INS OPS HRF-MELFI-R/INS OPS OR HRF-GLCR-R/INS OPS HRF-URINE-SETUP OR HRF-URINE-SETUP1 (1 st performance/subject) REMINDER-HRF URN END (post-sleep) HRF-URINE-OPS_SUB (post-sleep) HRF-URINE-OPS_SUB REMINDER-HRF URN CLT (pre-sleep) :05:20:05 :20 :05 HRF Generic Urine Collection :05 Crew TEXTFlexible Session Day -1 (Day 4/Start) Session Day -2 (Day 3/Day Before) Session Day (Day 5/Closeout)  FD15 (+/- 5 days), FD30 (+/- 14 days), FD60 (+/- 14 days), FD120 (+/- 14 days), and FD180/R-14 (+14/-0 days) sessions required per subject.  The start of the 24-hour urine collection window occurs 24 hours before blood collection. FD session windows correspond to blood collection day.  If urine ops/inserts are scheduled on off-duty days, schedule 3 urine ops/inserts on first day of collection and 2 urine ops/inserts on second day of collection.  Urine collection sessions are combined with concurrent sessions for Biochem Profile and/or Repository when subjects also participate in these experiments. When combined, refer to this slide.  Urine collection sessions should be combined with concurrent sessions for Salivary Markers when subjects also participate in that experiment, when possible. When combined, refer to this slide.  Urine collection sessions for multiple subjects should be scheduled concurrently or on consecutive days when possible. HRF-SAMPLE-PHOTO (one FD session/subject, any photo camera operator) HRF-MELFI-INS OPS OR HRF-GLCR-INS OPS HRF-MELFI-INS OPS OR HRF-GLCR-INS OPS :05

Revision G 8/22/13 PARC/T. Cassady Pro K Session Day -2 (Day 3) Session Day -5 (Day Before) Session Day -4 (Day 1/Start) Session Day -3 (Day 2) Session Day (Day 5/Closeout) :10 PROK-URN PH-TEST_SUB (post-sleep) PROK-DIET C-LOG1_SUB (midday) OR PRK-DIET M-LOG1_SUB (FD30 session midday) :05 PROK-LOG-PHOTO :05:10:05 PROK-DIET C-LOG_SUB (midday) OR PROK-DIET M-LOG_SUB (FD30 session midday) PROK-URN PH-TEST_SUB (post-sleep) PROK-DIET C-LOG_SUB (midday) OR PROK-DIET M-LOG_SUB (FD30 session midday) PROK-URN PH-TEST_SUB (post-sleep) PROK-URN PH-TEST_SUB (post-sleep) PROK-URN PH-TEST_SUB (post-sleep) :05 REMINDER-PROK DIET-C (pre-sleep) OR REMINDER-PROK DIET-M (FD30 session pre-sleep) PROK-PH KIT-UNSTOW PROK-DIET C-LOG_SUB (midday) OR PROK-DIET M-LOG_SUB (FD30 session midday) Session Day -1 (Day 4) Controlled Diet and Log and Urine pH Test Crew TEXTFlexible  FD15 (+/- 5 days), FD30 (+/- 14 days), FD60 (+/- 14 days), FD120 (+/- 14 days), and FD180/R-14 (+14/-0 days) sessions required per subject.  The start of the 4-day controlled diet (monitored FD30) window occurs 96 hours before blood collection, 72 hours before 24-hour urine collection start. FD session windows correspond to blood collection day.

Revision G 8/22/13 PARC/T. Cassady Pro K :30 HRF1-SLAMMD-BMM_SUB (all subjects, sequentially, in any order) HRF1-SLAMMD-SETUP OR HRF1-SLAMMD-SETUP1 OR (1 st time operator performance) HRF1-SLAMMD-SU CTRL OR HRF1-SLAMMD-SU CTRL1 OR (control run performed) (control run performed, 1 st time operator performance) HRF1-SLAMMD-SU PARTL OR HRF1-SLAMMD-SU PRTL CR (SLAMMD partially deployed) (SLAMMD partially deployed, control run performed) HRF1-SLAMMD-STOW OR HRF1-SLAMMD-STOW TMP OR HRF1-SLMD-STW TMP CR (SLAMMD left partially deployed) (SLAMMD left partially deployed, control run performed) HRF1-SLAMMD-CTRL RUN (control run performed, assume 1 st session/increment) :20 OR :30 OR :15 OR :20 :15:10 :20 OR :10 OR :20 HRF1-SLAMMD-TRACK SLAMMD Body Mass Measurement Crew Commanding Unattended HRF1-RACK-PWRDN CMD ESA-HRF2-DEACT (ESA) ESA-HRF1-ACT (ESA) HRF1-RACK/PC-TRACK 1:00 HRF1-RACK-PWRUP CMD  FD14 and every 30 days thereafter (all once/month) sessions required per subject.  SLAMMD BMM sessions are combined with concurrent sessions for Biochem Profile and Body Measures. When combined, refer to this slide.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Baseline27/2812/8/10 EO10-PPO Baseline27/28 & Subs 4/1/11 Nutrition Nominal: Reserve: 0.75 Pro K Nominal: 1.83 Reserve: 0.00 Repository Nominal: 3.25 Reserve: 0.00 Inc 27/28 Working PTP 11/1/10 Nutrition Nominal: Reserve: 0.75 Pro K Nominal: 1.67 Reserve: 0.00 Repository Nominal: 3.25 Reserve: 0.00 Nutrition Nominal: -Coleman (I27 FE6): 2 sessions and 2 monthly SLAMMD BMMs. - Nespoli (I27 FE5): 2 sessions and 2 monthly SLAMMD BMMs. - Garan (I27/28 FE3): 5 sessions and 6 monthly SLAMMD BMMs (includes 1 cntrl run). - Fossum( I28 FE6): 3 sessions and 4 monthly SLAMMD BMMs. - Furukawa (I28 FE5): 3 sessions and 4 monthly SLAMMD BMMs. Reserve: Photo ops (Garan, Furukawa, & Fossum). Pro K Nominal: Nespoli (I27 FE5): 2 sessions w/Nutrition. Reserve: N/A Repository Nominal: - Coleman I27 FE6): 2 sessions w/Nutrition. - Nespoli (I27 FE5): 2 sessions w/Nutrition. - Garan (I27/28 FE3): 5 sessions w/Nutrition. - Fossum (I28 FE6): 3 sessions w/Nutrition. - Furukawa (I28 FE5): 3 sessions w/Nutrition. Reserve: N/A PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision A29/30 & Subs 6/23/11 Nutrition Nominal: Reserve: 0.25 Pro K Nominal: Reserve: 0.25 Repository Nominal: 2.83 Reserve: 0.00 Inc 29/30 Working PTP 4/22/11 Nutrition Nominal: Reserve: 0.25 Pro K Nominal: Reserve: 0.00 Repository Nominal: 2.83 Reserve: 0.00 Nutrition Nominal: - Fossum (I29 CDR): 2 sessions (27S FD120 & FD180) and 2 SLAMMD sessions (monthly). - Furukawa (I29 FE5): 2 sessions (27S FD120 & FD180) and 2 SLAMMD sessions (monthly). - Pettit (I30 FE6): 3 sessions (29S FD15, FD30, & FD60) and 4 SLAMMD sessions (monthly) with Control Run included in first session. Reserve: Photo ops (Pettit). Pro K Nominal: - Pettit (I30 FE6): 3 sessions w/Nutrition (29S FD15, FD30, & FD60). - Kuipers (I30 FE5): 3 sessions (29S FD15, FD30, & FD60) and 4 SLAMMD sessions (monthly). Reserve: Photo ops (Kuipers). Repository Nominal: - Fossum (I29 CDR): 2 sessions w/Nutrition (27S FD120 & FD180). - Furukawa (I29 FE5): 2 sessions w/Nutrition (27S FD120 & FD180). - Pettit (I30 FE6): 3 sessions w/Nutrition (29S FD15, FD30, & FD60). - Kuipers (I30 FE5): 3 sessions w/Pro K (29S FD15, FD30, & FD60). - UMS Maintenance Flush: 4 sessions. Reserve: N/A PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision B31/32 & Subs 12/8/11 Nutrition Nominal: Reserve: 0.25 Pro K Nominal: Reserve: 0.50 Repository Nominal: 1.25 Reserve: 0.00 Inc 31/32 Working PTP 10/31/11 Nutrition Nominal: Reserve: 0.25 Pro K Nominal: Reserve: 0.50 Repository Nominal: 3.25 Reserve: 0.00 Nominal: - Kuipers (I31 FE5): 2 Nutrition/Repository/Pro K sessions (29S FD120 & FD180) and 2 SLAMMD sessions (monthly). (Crewtime booked under Pro K/Repository). - Pettit (I31 FE6): 2 Nutrition/Repository/Pro K sessions (29S FD120 & FD180) and 2 SLAMMD sessions (monthly). (Crewtime booked under Nutrition/Repository). - Acaba (I31/32 FE3): 5 Nutrition/Repository/Pro K sessions (30S FD15, FD30, FD60, FD120, & FD180) and 6 SLAMMD sessions (monthly). (Crewtime booked under Pro K/Repository). - Williams (I32 FE5): 3 Nutrition/Repository/Pro K sessions (31S FD15, FD30, & FD60) and 4 SLAMMD sessions (monthly) with Control Run included in first session. (Crewtime booked under Nutrition/Repository). - Hoshide (I32 FE6): 3 Nutrition/Repository/Pro K sessions (31S FD15, FD30, & FD60) and 4 SLAMMD sessions (monthly). (Crewtime booked under Pro K/Repository). - Nutrition PTP crewtime does not include first-time increases for SLAMMD ops (additional 10 min/initial setup performance/crew; assume one initial setup/inc). - Crewtime does not include UMS Prefilter Replace ops (the crewtime will be included once UMS is operational). - Crewtime for UMS Maintenance Flush ops is included in the UMS crew roll-up. Reserve: Photo ops (Williams, Acaba, & Hoshide). PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision C33/34 & Subs 6/28/12 Nutrition Nominal: Reserve: 0.25 Pro K Nominal: Reserve: 0.50 Repository Nominal: 1.25 Reserve: 0.00 Inc 33/34 Working PTP 5/11/12 Nutrition Nominal: Reserve: 0.00 Pro K Nominal: Reserve: 0.50 Repository Nominal: 1.25 Reserve: 0.00 Nutrition: 4.33 hrs/subject/FD session, no crewtime when performed with Pro K; add 0.83 hrs/subject/SLAMMD session, 0.17 hrs for initial SLAMMD session (time increase for first performance), and 0.25 hrs for control run; add 0.25 hrs/subject for photos. Nominal: - Ford (I33/34 FE3/CDR): 5 FD sessions (32S FD15, FD30, FD60, FD120, and FD180/R-14) and 6 SLAMMD sessions (monthly) with setup/stow per session and control run in first session. - Williams (I33 CDR): 2 FD sessions w/Pro K (31S FD60/FD120 and FD180/R-14). - Hoshide (I33 FE6): 2 FD sessions w/Pro K (31S FD60/FD120 and FD180/R-14). - Hadfield (I34 FE5): 3 FD sessions w/Pro K (33S FD15, FD30, and FD60). - Marshburn (I34 FE6): 3 FD sessions w/Pro K (33S FD15, FD30, and FD60). Reserve: Photos: Ford (I33/34 FE3/CDR). Pro K: 5.25 hrs/subject/FD session; add 0.17 hrs/subject/SLAMMD session; add 0.25 hrs/subject for photos. Nominal: - Williams (I33 CDR): 2 FD sessions (31S FD60/FD120 and FD180/R-14) and 2 SLAMMD sessions (monthly). - Hoshide (I33 FE6): 2 FD sessions (31S FD60/FD120 and FD180/R-14) and 2 SLAMMD sessions (monthly). - Hadfield (I34 FE5): 3 FD sessions (33S FD15, FD30, and FD60) and 4 SLAMMD sessions (monthly). - Marshburn (I34 FE6): 3 FD sessions (33S FD15, FD30, and FD60) and 4 SLAMMD sessions (monthly). Reserve: Photos: Hadfield (I34 FE5) and Marshburn (I34 FE6). Repository: 0.08 hrs/subject/FD session when performed with Nutrition or Pro K. Nominal: - Williams (I33 CDR): 2 FD sessions w/Pro K (31S FD60/FD120 and FD180/R-14). - Hoshide (I33 FE6): 2 FD sessions w/Pro K (31S FD60/FD120 and FD180/R-14). - Ford (I33/34 FE3/CDR): 5 FD sessions w/Nutrition (32S FD15, FD30, FD60, FD120, and FD180/R-14). - Hadfield (I34 FE5): 3 FD sessions w/Pro K (33S FD15, FD30, and FD60). - Marshburn (I34 FE6): 3 FD sessions w/Pro K (33S FD15, FD30, and FD60). Reserve: N/A PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision D33/34 & Subs 8/30/12--- OCR hops…1063 incorporated (3x5 Mesh Bag Usage). - GJOP Action incorporated (De-chunkify HRF Generic Blood Collection). - No change to crew roll-up from last revision. EO10-PPO Revision E35/36 & Subs 12/19/12 Pro K Nominal: Reserve: 0.75 Nutrition Nominal: 0.00 Reserve: 0.00 Repository Nominal: 1.00 Reserve: 0.00 Inc 35/36 Working PTP 10/24/12 Pro K Nominal: Reserve: 0.75 Nutrition Nominal: 0.00 Reserve: 0.00 Repository Nominal: 1.00 Reserve: 0.00 Pro K: 5.42 hrs/subject/FD session, 0.25 hrs/subject for photo ops; 0.83 hrs/SLAMMD session for one subject, 0.17 hrs for each additional subject on the same day, 0.17 hrs for first time SLAMMD session (time increase for first performance), 0.25 hrs for control run. Nominal: - Hadfield (I35 CDR): 2 FD sessions (33S FD120, FD180/R-14) and 2 SLAMMD sessions (monthly). - Marshburn (I35 FE6): 2 FD sessions (33S FD120, FD180/R-14) and 2 SLAMMD sessions (monthly). - Cassidy (I35/36 FE3): 5 FD sessions (34S FD15, FD30, FD60, FD120, FD180/R-14) and 6 SLAMMD sessions (monthly) with control run in first session. - Parmitano (I36 FE5): 3 FD sessions (35S FD15, FD30, FD60) and 4 SLAMMD sessions (monthly). - Nyberg (I36 FE6): 3 FD sessions (35S FD15, FD30, FD60) and 4 SLAMMD sessions (monthly). Reserve: -Photo ops: Cassidy (I35/36 FE3), Nyberg (I36 FE5), and Parmitano (I36 FE6). Nutrition: No additional time when performed with Pro K. Nominal (0.00 hrs for I35/36): - Marshburn (I35 FE6): 2 FD session w/Pro K (33S FD120, FD180/R-14). Reserve (0.00 hrs for I35/36): - N/A Repository: 0.08 hrs/subject/FD session when performed with Pro K or Nutrition. Nominal: - Hadfield (I35 CDR): 2 FD sessions w/Pro K (33S FD120, FD180/R-14). - Marshburn (I35 FE6): 2 FD sessions w/Pro K (33S FD120, FD180/R-14). - Cassidy (I35/36 FE3): 5 FD sessions w/Pro K (34S FD15, FD30, FD60, FD120, FD180/R- 14). - Parmitano (I36 FE5): 3 FD sessions w/Pro K (35S FD15, FD30, FD60). Reserve: -N/A PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision F35/36 & Subs 5/6/13 Pro K Nominal: Reserve: 0.75 Nutrition Nominal: 0.00 Reserve: 0.00 Repository Nominal: 0.17 Reserve: 0.00 Inc 35/36 Working PTP 2/19/13 Pro K Nominal: Reserve: 0.75 Nutrition Nominal: 0.00 Reserve: 0.00 Repository Nominal: 1.00 Reserve: 0.00 Ops: - Pro K (FD15, FD30, FD60, FD120, & FD180/R-14): 5.50 hrs/session, additional 0.25 hrs for photos (one session/subject). - Nutrition (FD15, FD30, FD60, FD120, & FD180/R-14): 0.00 hrs/session when performed with Pro K. - Repository (FD15, FD30, FD60, FD120, & FD180/R-14): 0.00 hrs/session when performed with Pro K; 0.08 hrs/session/subject when performed with Nutrition. - SLAMMD BMM (once/month): 0.83 hrs/session for one subject, 0.17 hrs for each additional subject on the same day, additional 0.17 hrs for 1 st SLAMMD session (time increase for 1 st performance), additional 0.25 hrs for control run (once/Inc during 1 st monthly session). Nominal: - Hadfield (33S CDR): FD120 and FD180/R-14 ProK/Repo sessions and 2 monthly SLAMMD sessions (1x/month). - Marshburn (33S FE6): FD120 and FD180/R-14 ProK/Nutr/Repo sessions and 2 monthly SLAMMD sessions (1x/month). - Cassidy (34S FE3): FD15, FD30, FD60, FD120, and FD180/R-14 ProK/Repo sessions and 6 monthly SLAMMD sessions (1x/month) with control run during 1st monthly session. - Parmitano (35S FE5): FD15, FD30, and FD60 ProK/Repo sessions and 4 monthly SLAMMD sessions (1x/month). - Nyberg (I35S FE6): FD15, FD30, and FD60 ProK sessions and 4 monthly SLAMMD sessions (1x/month). Reserve: - Photos: Cassidy (34S FE3), Nyberg (35S FE5), and Parmitano (35S FE6). PPO Change Log *Crew Roll-up is not maintained real-time.

Revision G 8/22/13 PARC/T. Cassady Pro K Change#RevisionIncDateCrew Roll-up* (hrs) PTP (hrs) Crew Roll-up Notes EO10-PPO Revision G37/38 & Subs 8/22/13 Pro K Nominal: Reserve: 0.25 Repository Nominal: 0.67 Reserve: 0.00 Inc 37/38 Working PTP 2/19/13 Pro K Nominal: Reserve: 0.25 Repository Nominal: 0.92 Reserve: 0.00 Ops: - Pro K Blood Collection and Urine Collection (FD15, FD30, FD60, FD120, FD180/R-14): 4.17 hrs/session, 0.25 hrs for HISD photo (one FD session/subject). - Pro K Controlled Diet and Log and Urine pH Test (FD15, FD30, FD60, FD120, FD180/R- 14): 1.08 hrs/session. - Pro K SLAMMD Body Mass Measurement (FD14, every 30 days thereafter): 0.83 hrs/session for one subject, 0.17 hrs for each additional subject on the same day, 0.17 hrs for first time operator setup performance (assume once/increment), 0.25 hrs for control run (once every 6 months). Nominal: - Parmitano (35S FE5): FD120, FD180/R-14 ProK/Repo blood/urine sessions and ProK diet/log/pH sessions; 2 monthly ProK SLAMMD sessions (once/month; crewtime for setup/stow carried under Hopkins). - Nyberg (35S FE6): FD120, FD180/R-14 ProK blood/urine/diet/log/pH sessions; 2 monthly ProK SLAMMD sessions (once/month; crewtime for setup/stow carried under Hopkins). - Hopkins (36S FE3): FD15, FD30, FD60, FD120, FD180/R-14 ProK/Biochem/Repo blood/urine sessions and ProK diet/log/pH sessions; 3 monthly ProK/Biochem/BodyM and 3 monthly ProK/Biochem SLAMMD sessions, 6 total (once/month; crewtime for setup/BMM/stow carried by Pro K), control run (1st monthly session). Reserve: - HISD photo: Hopkins (36S FE3). *Crew Roll-up is not maintained real-time. PPO Change Log