Baseline 06/10/10 TDC Combination Protocol Overview Controlled/Monitored Diet Ops Urine Ops 5 Sessions/Subject: FDs 15, 30, 60, 120, & 180; FDs correspond to blood collection day: FD15 scheduled +/- 5 days All other FDs scheduled +/- 14 days except: - No less than 7 days between FD15 and FD30 - No less than 3 weeks between other FDs If mission not exactly 180 days, final session scheduled within 2 weeks of landing, but greater than 3 weeks from previous session Day 1 Urine Setup Urine pH Testing Diet Logging Urine pH Testing Diet Logging Urine pH Testing Diet Logging Urine pH Testing Diet Logging 24-Hr Urine Collection (day 1) Urine pH Testing Closeout 24-Hr Urine Collection Closeout (day 2) Blood Collection & Processing Diet & pH Log Photo Blood Setup Blood Ops Day 2Day 3Day 4Day 5 Nutrition - Repository - PRO K Diet & pH Log Photo OCA Downlink Blood Stow Urine Stow

Baseline 06/10/10 TDC PRO K Crew Ground 5 Sessions/Subject: Scheduled for each FD session (FDs 15, 30, 60, 120, & 180): Scheduled over 5 consecutive days beginning 96 hrs prior to blood ops and 72 hrs prior to start of 24-hr urine ops (4-day diet period: days 1 - 4; diet closeout: day 5): - Urine pH testing scheduled daily preferably in post-sleep (or at same time of day) for 5 consecutive days during 4-day diet period and diet closeout day - Diet logging scheduled daily mid-day during 4-day diet period - Diet & pH test log photos scheduled at end of session on diet closeout day (after urine and blood ops) - OCA downlink scheduled ASAP after photos Minimum 1-week separation between SOLO and PRO K sessions PROK-URN PH-TEST_SUB PROK-DIET-LOG1_SUB 4-Day Controlled/Monitored Diet Urine pH Testing & Diet Logging :05 Day 1 1st Day of Diet Day 5 Closeout Day of Diet Day 2 2nd Day of Diet Day 3 3rd Day of Diet Day 4 4th Day of Diet PROK-URN PH-TEST_SUB PROK-DIET-LOG2-4_SUB PROK-URN PH-TEST_SUB PROK-DIET-LOG2-4_SUB PROK-URN PH-TEST_SUB PROK-DIET-LOG2-4_SUB PROK-URN PH-TEST_SUB PROK-LOG-PHOTO_SUB :15 PROK-OCA-DNLK :05

Baseline 06/10/10 TDC 24-Hour Urine Sample Collection 5 Sessions/Subject: Scheduled for each FD session (FDs 15, 30, 60, 120, & 180): Scheduled over 24-hr period (first morning void on day 1 through post-sleep void on day 2 to capture first morning void on both days); For planning purposes, 5 voids per crew per 24-hr period chosen as basis of calculation: - First and last urine collects/inserts of each 24-hr period scheduled in post-sleep - CS represents Cold Stowage unit: CS = MELFI for MELFI; CS = GLCR for Glacier - If urine ops scheduled on off-duty days, use 3 urine collect/insert placeholders on day 1 and 2 urine collect/insert placeholders on day 2 to ensure 24 hrs of collection For NUTR/REP/PROK combined sessions (X = A): Scheduled to begin 72 hrs after start of 4-day diet period and 24 hrs prior to blood ops For NUTR/REP combined sessions (X = B): Scheduled to begin within 24 hrs of blood ops Crew Nutrition - Repository - PRO K Once/Subject (any session) Day prior to 24-Hr Collection start HRF-URINE-STOW 1st Day of 24-Hr Collection2nd/Closeout Day of 24-Hr Collection HRF-URINE-SETUP :20 3 times throughout the day :20:05 24 hrs HRF-URN-CLCT_SUB_X HRF-URINE CS-INS :20:05 HRF-URN-CLCT_SUB_X HRF-URINE CS-INS HRF-URN-CLCT_SUB_X HRF-URINE CS-IN1 :10:20 :05 NUTR-HISD-PHOTO

Baseline 06/10/10 TDC HRF-BLOOD-COAG Day prior to Blood Collection :20 HRF-BLOOD-SETUP_X ESA-HRF2-ACT CMD PRO-HRF2-ACT CMD HRF2-RACK-PWRUP CMD HRF2-RC-TRACK HRF2-RACK/PC-TRACK HRF-BLOOD-STOW Blood Collection & Processing Day Blood Sample Collection & Processing Nutrition - Repository - PRO K < 1 hr HRF-BLOOD-DRAW_OPR HRF-BLOOD CS-INS HRF-RC-CNFG_X HRF2-RC-SPIN 30 HRF-SPIN-CNCLD HRF-RC-SETUP_X :05 :15 :20:10 :30:15 :05 :10 1:00 NUTR-HISD-PHOTO Crew Ground Time Critical Optional (Subject preference) Once/Subject 5 Sessions/Subject: Scheduled for each FD session (FDs 15, 30, 60, 120, & 180): Scheduled on FD session day: - Blood draws: Scheduled in post-sleep; Not scheduled two weeks prior to EVA unless approved and supervised by crew surgeon or his/her designee - CS represents Cold Stowage unit: CS = MELFI for MELFI; CS = GLCR for Glacier For NUTR/REP/PROK combined sessions (X = A): Scheduled 96 hrs after start of 4-day diet period and 24 hrs after start of 24-hr urine ops For NUTR/REP combined sessions (X = B): Scheduled within 24 hrs of 24-hr urine ops start :10 1:00 :30 HRF2-RACK-PWRDN CMD PRO-HRF2-DEACT CMD ESA-HRF2-DEACT CMD HRF-BLOOD-DRAW_SUB_X :10 :15 HRF-PHOTO OCA-DNLK Scheduled within 5 days after Nutrition photo series (3 photos: 1 for urine ops & 2 for blood ops)