동물 분자 유전체 연구의 최신 동향 National Institute of Animal Science Animal Genomics & Bioinformatics 정호영

Genome analysis I Gene expression II Bioinformatics IV Metagenomics III Content

Genome analysis I

Association test Prediction Genomic selection Chromosomal study Linkage analysis Genetic variants In/del situation Gene chips Copy number variation DNA/RNA Chromosome Phenotypes Genome analysis

Genetic identification 개체식별 체계  친자일 경우의 수를 확률로 나타냄 (2 N )  ABO 식 혈액형 이외에 - Restriction fragment length polymorphism (RFLP) - Single strand conformational polymorphism (SSCP) - MS (Microsatellite markers) - SNP (Single nucleotide polymorphism) III AA ? AB  가축의 등록  종모우 선정 Genome analysis

Insertion/Deletion 열성유전자 확인  특정 질병관련 유전자 확인  유전병 진단 Genome analysis

Gene chip Genome wide 및 차등 발현 유전자 탐색  Microarray 이용 차등 발현확인  Gene pathway 구축 및 확인 probe (on chip) sample (labelled) Genome analysis

Microarray chip  Approximately 60 bp long  Expression study Genome analysis

SNP chip  Less than 25 bp long  Genome study Genome Wide Association Study (GWAS) analysis  777,000 SNP  50,000 SNP  8,000 SNP  ? Genome analysis

Copy Number Variation ATTG ACAGTA ACAGTA Genome analysis

Copy Number Variation Autism: De novo deletions more common in patients with Autism. New Syndrome: Deletion on chromosome 17 defines novel genetic disorder. Aids: CNV covering CCL3L1, large copy number is protective of HIV/AIDS infection. Association with Crohn’s disease and BMI. Genome analysis

Figure. A digital array has 12 panels of 765 reaction chambers each. Dube S, Qin J, Ramakrishnan R (2008) Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device. PLoS ONE 3(8): e2876. doi: /journal.pone Genome analysis

Figure. Human genomic DNA NA10860 (left 5 panels) and the RPP30 synthetic construct (right 5 panels) were quantitated using the RPP30 (FAM) assay on this digital array. Dube S, Qin J, Ramakrishnan R (2008) Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device. PLoS ONE 3(8): e2876. doi: /journal.pone Genome analysis

Linkage analysis

Genome analysis

Ahmad T et al. Hum. Mol. Genet. 2003;12: Genome analysis

Gene expression II

Gene expression 차등 발현 유전자 탐색  Microarray 이용 차등 발현 확인  Gene pathway 구축 및 확인 Gene expression

probe (on chip) sample (labelled) Gene expression  Approximately 60 – 250 bp long  Expression study  Big opportunity for everyone

Fluorescence in situ hybridization (FISH) Gene expression

Real time PCR Gene expression

Real time PCR Gene expression

Genetic association: Improvement of accuracy for selecting sires. Prediction of phenotypic performance would be possible. - Various prediction models for genetic diseases As results, genomic selection can be done. - Economic benefitsAssociation

Metagenomics III

Comparisons MRO + grain- based Milk replacer only (MRO), MRO + orchard grass hay Control(MG; Bel Air Calf Starter 22%, Bel Air, MD; 22.0% CP, 4.0% crude fat; 5.5% crude fiber), Fed free choice Changing circumstances of rumen Metagenomics

M vs G M vs H

Metagenomics

Epigenetics Question: Genetic and Environmental effects

Bioinformatics IV

Bioinformatics Systems Biology

당부의 말

젊음젊음 도전도전 창의창의 열정열정 용기용기 자신을 위해 도전하는 나

대한민국의 연구 수준은 선진국과 별 차이점이 없다. 하지만 연구에 대한 생각은 매우 다르다. 세계 최고가 되고자 하지 말고 항상 합리적, 논리적 사고를 하자 세계 최고가 되고자 하지 말고 항상 합리적, 논리적 사고를 하자