MBV2010 Colloquium, February 11

RULES Multiple choice Only one correct answer seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

Example Below is a series of equations. Which numbers would fit logically into the last equation ? (13 – 4) : 9 = 1(27 – 9) : 9 = 2 (87 – 15) : 9 = 8(68 - ??) : 9 = ?? A( ) : 9 = 6 B (68 - 5) : 9 = 7 C ( ) : 9 = 5 D ( ) : 9 = 4 0

Example Below is a series of equations. Which numbers would fit logically into the last equation ? (13 – 4) : 9 = 1(27 – 9) : 9 = 2 (87 – 15) : 9 = 8(68 - ??) : 9 = ?? 0

Dideoxynucleotides are used in A DNA sequencing B Cloning of DNA C PCR D Southern hybridization 1

Dideoxynucleotides are used in 1

Ribosomal RNAs A code for ribosomal proteins B function in transcription of genes C are absent from mitochondria D function in protein synthesis 2

Ribosomal RNAs 2

The smallest human chromosome has a size of approximately A 32 x 10 6 bp B 41 x 10 6 bp C 47 x 10 6 bp D 58 x 10 6 bp 3

The smallest human chromosome has a size of approximately 3

The polymerase chain reaction (PCR) is used for A amplifying proteins B copying plasmids C copying RNA D amplifying DNA 4

The polymerase chain reaction (PCR) is used for 4

DNA ligases A catalyze the formation of ß-N- glycosidic bonds B play a role in transcription of DNA C catalyze the formation of phosphodiester bonds D catalyze the formation of hydrogen bonds 5

DNA ligases 5

Which of the following is not found in RNA ? A G-U base pairing B ribonucleotides C double helices D supercoiling 6

Which of the following is not found in RNA ? 6

What is shown in this figure? A a random RNA structure B a microRNA structure C a small nuclear RNA structure D a tRNA structure 7

What is shown in this figure? 7

Protein structures can be determined by A mass spectrometry B 2D-polyacrylamide gel electrophoresis C x-ray diffraction D liquid chromatography 8

Protein structures can be determined by 8

The amino acid sequence of proteins can be determined by A mass spectrometry B 2D-polyacrylamide gel electrophoresis C x-ray diffraction D liquid chromatography 9

The amino acid sequence of proteins can be determined by 9

Centromeres A are present in all chromosomes B are normally in the center of chromosomes C are nucleosome-free sequences D normally contain repetitive DNA 10

Centromeres 10

The average size of genes in bacterial genomes is 11 A 1000 – 1500 bp B 500 – 1000 bp C 3000 – 4000 bp D 1500 – 3000 bp

The average size of genes in bacterial genomes is 11 Vibrio cholerae Deinococcus radiodurans (Conan the bacterium)

Microsatellites are 12 A genome-wide repeats B up to 1000 bp in length C found at the ends of chromosomes D used in genetic profiling

Microsatellites are 12

What is shown in this picture ? 13 A a metaphase chromosome B an anaphase chromosome C two homologous chromosomes D a chromosome before replication of DNA

What is shown in this picture ? 13 Chromatid Centromere