Innovative solutions. Measurable results. equipment products targets Environmental Professional Contracting Services Dale Anderson EPCS Environmental LLC July 1, 2008 EPCS Environmental LLC

Innovative solutions. Measurable results. equipment products targets Biofilm New Health Risk EPCS Environmental LLC

2 © 2007 MaxSpray International Corporation Kill Attacking Amoebae ScienceDaily (July 25, 2008) — Bacteria rarely come as loners; more often they grow in crowds and squat on surfaces where they form a community together. These so-called biofilms develop on any surface that bacteria can attach themselves to. The dilemma we face is that neither disinfectants and antibiotics, nor phagocytes and our immune system can destroy these biofilms. Environmental Professional Contracting Services  Amoebae normally attack and kill harmful pathogens  Pathogens have become Resistant (biofilm bacteria use chemical weapons to defend themselves.)  They clump together and build a slimy fortress of defense  Effective in the presence of all amoebae, killing them or causing them to become de-activated  Athletic Facilities  Hospitals  Implants  Catheters  Surfaces  Surgical equipment Common Problem Areas

3 © 2007 MaxSpray International Corporation ÆGIS/mPale … Microbe Shield  Microbe Shield Program based on a unique antimicrobial technology which effectively controls a broad spectrum of bacteria, fungi, algae and yeasts on a wide variety of treated substrates  Used safely and effectively for 30 years  Tested and approved for use in EPCS electrostatics sprayers Environmental Professional Contracting Services

4 © 2007 MaxSpray International Corporation ÆGIS/mPale … Microbe Shield The Mark of Excellence FEATURESBENEFITS Non-LeachingDoes not migrate Apply with ElectrostaticsEfficient and timely application process Permanent ApplicationBONDS to surface EPA Registered in 1976Complies to strict Federal guidelines SAFERenders many microorganisms lifeless via an electrochemical process Provides Water Repellency Intrinsic feature that helps preserve the substrate Non-FlammableSafe for all surfaces OdorlessDoes not cause allergic reactions WarrantyAvailable against mold/mildew growth Environmental Professional Contracting Services

5 © 2007 MaxSpray International Corporation TreatedNot Treated Photographs taken by electronic microscope ÆGIS/mPale Microbe Shield Treatment Staphylococcus aureus ( MRSA )

6 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services ÆGIS/mPale … Microbe Shield Representative Microorganisms Tested: A Partial Compendium BACTERIAEscherichia coliBacillus subtilis Micrococcus sp.Proteus mirabilisBacillus cereus Staphylococcus epidermidisProteus mirabillisClostridium perfringens Enterobacter agglomeransCitrobacter diversusHaemophilus influenzae Acinetobacter calcoaceticusSalmonella typhosaHaemophilus suis Staphylococcus aureus (pigmented) Salmonella choleraesuisLactobacillus casei Staphylococcus aureus (non-pigmented) Corynebacterium bovixLeuconostoc lactis Klebsiella pneumoniae ATCC 4352 Mycobacterium smegmatisListeria monocytogenes Pseudomonas aeruginosaMycobacterium tuberculosisPropionibacterium acnes Pseudomonas aeruginosaBrucella caniaProteus vulgaris Pseudomanas aeruginosa PDR-10 Brucella abortusPseudomonas cepacia Streptococcus faecalisBrucella suisPseudomonas fluorescens Escherichia coli ATCC 23266Streptococcus mutansXanthomonas Campestris

7 © 2007 MaxSpray International Corporation ÆGIS/mPale … Microbe Shield Representative Microorganisms Tested: A Partial Compendium (cont’d) FUNGIMucor sp.ALGAE Aspergillus nigerTricophyton mentagrophytes Oscillatoria borneti LB143 Aspergillus fumigatusTricophyton interdigitalieAnabaena cylindrica B C Aspergillus versicolorTrichoderma flavusSelenastrum gracile B-325 Aspergillus flavusChaetomium globusumPleurococcus sp. LB11 Aspergillus terreusRhizopus nigricansSchenedesmus quadricauda Penicillium ChrysogenumCladosporium herbarumGonium sp. LB 9c Penicillium albicansAerobasidium pullulansVolvox sp. LB 9 Penicillium citrinumFusarium nigrumChlorella vulgarus Penicillium elegansFusarium solani Penicillium funiculosumGliocladium roseumYEAST Penicillium humicolaOospora lactisSaccharomyces cerevisiae Penicillium notatumStachybotrys atraCandida albicans Penicillium variabile Environmental Professional Contracting Services

8 © 2007 MaxSpray International Corporation The ÆGIS/mPale Microbe Shield  Highly Effective  Long-Lasting  Odorless/Colorless  Extremely Low Toxicity  Long History of Safe Use  Permanent Chemical Bonding  Won’t Wash Off or Affect Skin Microflora Environmental Professional Contracting Services

9 © 2007 MaxSpray International Corporation mPerial™  Cleaner, sanitizer and deodorizer  Registered with the EPA as a germ killer and fungicide and is authorized for use in food preparation areas. It is specially formulated for compatibility with mPale Antimicrobial.  Virucide  Fungicide (against pathogenic fungi)  Mildewstat (on hard, inanimate surfaces)  Effective in the presence of 5% serum contamination  For home, hospital, institutional and industrial use, school, restaurant, food handling and processing areas  Kills germs Environmental Professional Contracting Services

10 © 2007 MaxSpray International Corporation ÆGIS/mPale  mPerial™ Treatment  All touch surfaces Fountain area for drinks and ice Carpet and floor mats Doorknobs, handrails Rest rooms Play ground support products Environmental Professional Contracting Services

11 © 2007 MaxSpray International Corporation ÆGIS/mPale  mPerial™ Treatment  New construction Ceilings Floors Walls Wall cavities Above ceiling space Chases Furnishings A/C ducts

12 © 2007 MaxSpray International Corporation ÆGIS/mPale  mPerial™ Treatment  Transportation: Trucks and Trailers Reduces bacteria load Reduces odors Makes treated surfaces easier to clean Tremendous value to cost ratio Tagging agent built-in to verify validity Environmental Professional Contracting Services

Innovative solutions. Measurable results. equipment products targets Evaluation of a Silane Quarternary Ammonium Salt as Antimicrobial Fabric Treatment Arvye Davis, Randall Sumner, SLSTP-Flight Group2004 EPCS Environmental LLC

14 © 2007 MaxSpray International Corporation Overview Antimicrobial agents are used to kill or limit growth of microorganisms. ÆGIS Microbe Shield™, an antimicrobial agent composed of a silane quaternary ammonium salt, is featured for its durability and efficacy against both gram-negative and gram-positive bacteria. This research studied the effectiveness of the antimicrobial agent as a treatment for cotton fabric. Cotton fabric samples were coated with the antimicrobial agent, then tested against three species of bacteria. The samples were shaken in a bacterial solution to allow contact with the microbes, and the number of viable bacteria were counted. The data were compared to the numbers of bacteria with untreated fabric. This research reveals that the antimicrobial agent reduced the numbers of bacteria exposed to the treated cotton fabric. Environmental Professional Contracting Services

15 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services Research  The three types of bacteria were Escherichia coli, Staphylococcus epidermidis, and Bacillus subtilus. For each test, the bacteria were grown in Tryptic Soy Broth (TSB) for eighteen hours at room temperature. The bacteria were diluted in 0.003M KH 2 PO 4 to 72 percent transmission using a Vitek Colorimeter. For testing, the bacteria were diluted 1:1000 to x 10 5 cfu/mL. Then, 50mL aliquots of the diluted bacteria were distributed into sterile 250mL flasks.  The number of bacteria were determined by the pour plate method using Tryptic Soy Agar (TSA). The control was plated at “0” contact time. The nine flasks were agitated in a reciprocal shaker at 120 RPM. After one hour, all bacteria samples were plated and incubated for 24 hours at 35°C.

16 © 2007 MaxSpray International Corporation Results Graph 1: Comparison of all pure cultures used with and without the antimicrobial agent. It was discovered that the antimicrobial agent did exhibit the ability to kill the three species of bacteria. Acknowledgements This research was conducted as part of the 2004 Spaceflight and Life Sciences Training Program funded by the National Aeronautics and Space Administration. The authors recognize the support of the Dynamac Corporation, the NASA Spaceflight and Life Sciences Training Program Academic Partner Alliance and the United States Department of Agriculture. I would like to thank my Principal Investigator, Randall Sumner, for his hard work in preparation of this research, and I would also like to thank Jason Johns and Adam Santone for their help and guidance in this research. Environmental Professional Contracting Services

Innovative solutions. Measurable results. equipment products targets Electrostatic Spraying Technology EPCS Environmental LLC

Innovative solutions. Measurable results. equipment products targets Inefficiencies of Conventional Sprayers EPCS Environmental LLC

19 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services floor/ground drifts on target Inefficiencies of conventional sprayers  Of the total amount of product applied with conventional sprayers 60% to 70% goes on the floor/ground 15% to 20% drifts from immediate area only 15 to 20% is deposited on the target less than 3% contacts near the intended area under target

Innovative solutions. Measurable results. equipment products targets Advantages Electrostatic Technology EPCEPCS Environmental LLC

21 © 2007 MaxSpray International Corporation Air-Assisted Electrostatic Sprayers  The charged droplets seek out surfaces by electrostatic forces. The result is a uniform spray coverage in hidden areas: underside, crevices, holes, and empty spaces you can’t see with a naked eye. RESULTS: is over 30 to 45-fold increase to these hidden areas. Environmental Professional Contracting Services

22 © 2007 MaxSpray International Corporation Over 4 to 7-fold deposit efficiency improvement Provides uniform dispersion and coating Leaves no visible residue Reduces “hang-time” Coats air-borne and non-conductive targets OTHER BENEFITS: Using electrical charges to enhance the behavior of spray Environmental Professional Contracting Services

23 © 2007 MaxSpray International Corporation 1/30 th of a second Charged Spray Uncharged Spray Charged droplets move much faster to the target BENEFIT: Reduces “Hang-Time” Environmental Professional Contracting Services

24 © 2007 MaxSpray International Corporation Uncharged spray Charged spray Demonstration of Electrostatic Coating on Non-conductive Target BENEFIT: Reduces “hang-time”; Coats air-borne and non-conductive targets Environmental Professional Contracting Services

Innovative solutions. Measurable results. equipment products targets Electrostatics Demo “Black Ball” EPCS Environmental LLC

26 © 2007 MaxSpray International Corporation Black Ball Demo (The target object was sprayed from a distance of 3 meters; the black background was added to make the spray more visible.) First, we spray the sphere with electrostatics turned OFF: 1 – 2 – 3 – 4 seconds. Environmental Professional Contracting Services

27 © 2007 MaxSpray International Corporation Black Ball Demo Notice the coverage on the target. Environmental Professional Contracting Services

28 © 2007 MaxSpray International Corporation Black Ball Demo (All other settings are kept constant, so half the amount of material is sprayed.) Finally, we turn electrostatics ON, and spray a new sphere for half the time: 1 – 2 seconds. Environmental Professional Contracting Services

29 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services Black Ball Demo Notice the coverage on the target. The charged droplets wrapped around, reaching the back of the sphere.

30 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services Black Ball Demo Results Original Sphere Electrostatics OFF Electrostatics ON

Innovative solutions. Measurable results. equipment products targets Successful Applications Healthcare, Athletics/Sports Facilities, Food Industry, Correctional Facilities, Airline Industry, Schools/Colleges, Residential/Businesses EPCS Environmental LLC

32 © 2007 MaxSpray International Corporation Control of Microorganisms in Indoor Environments  Medical Centers / Hospitals / Hospices DEMO  Athletics / Sports Facilities Virginia Tech University  Correctional Facilities Metro Detention Center  Airline Industry  Schools / Colleges Over 75 school coated in 2006 & 2007  Residential / Businesses Mohawk - World’s largest floor manufacturer EXAMPLES Environmental Professional Contracting Services

33 © 2007 MaxSpray International Corporation Case Study Arthur G. James Cancer Hospital & Research Institute, Columbus, OH Lee Memorial Hospital, Lee County, FL Improved Control of Microbial Exposure in Hospitals: A 30 Month Field Study

34 © 2007 MaxSpray International Corporation Case Study Washington Redskins, Washington, D.C. Over 26 sports facilities coating in the last 22 months. Environmental Professional Contracting Services

35 © 2007 MaxSpray International Corporation Case Study Metro Detention Center Atlanta, GA Environmental Professional Contracting Services

36 © 2007 MaxSpray International Corporation Case Study Airlines Industry Test coating cabins of two 757’s Environmental Professional Contracting Services

37 © 2007 MaxSpray International Corporation Various Institutions: Environmental Professional Contracting Services  SwathmoreCollage  Atlanta Thrashers  Cal State Bakersfield  Stevens Institute of Technology  Columbia University  Grundy Wrestling  John Hopkins University  Virginia Tech  Ohio State University  University of South Carolina  University of Tulsa  Southern Illinois University  University of Illinois Southern  Missouri State

38 © 2007 MaxSpray International Corporation Environmental Professional Contracting Services Various Sports Facilities:  Miami Heat  Cleveland Browns  Washington Wizards  Atlanta Hawks  New Jersey Nets  Washington Redskins  Minnesota Vikings  Detroit Lions

39 © 2007 MaxSpray International Corporation The WOW F actors  Electrostatic technology = The 1 st Wow!  mPale  mPerial products = The 2 nd Wow!  Ability to measure the amount of mPale on the surface/target = The 3 rd Wow! $15M research was invested to discover these Wows! Environmental Professional Contracting Services

40 © 2007 MaxSpray International Corporation Three Step Game Plan  You need a plan designed to protect your sports facilities, equipment and synthetic turf fields.  The big advantages to the Three Step Game Plan  Less Invasive  More Effective  Faster  Lower Cost  Longer Lasting  More Versatile  More Efficient  Moisture Resistant Environmental Professional Contracting Services

41 © 2007 MaxSpray International Corporation Three Step Game Plan  Sophisticated Technologies  Advanced Application Protocols  More Affordable Than Ever  What's your game plan? Environmental Professional Contracting Services

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