การยับยั้งจุลินทรีย์โดยใช้วัสดุซิลเวอร์นาโน ที่ได้จากเคมีสีเขียวเพื่อประยุกต์ใช้ในการ บำบัดน้ำ ( Antibacterial Ability of Ag and Ag/AgCl Nanoparticles from Radish and Tea Extracts for Water Treatment Application ) น. ส. ยุภาดา บุญโต ดร. จิรภัทร์ อนันตภัทรชัย, รศ. ดร. พวงรัตน์ ขจิตวิชยานุกูล

Change for safe the world

Comparisons of conventional method and green synthesis for produce green nanomaterials

Plant extract Green tea thumb/d/d8/Caffeine.svg/764px-Caffeine.svg.png Caffeine Radish Folic acid

Nano Technology

Silver NPs Bactericidal vc/ImageService/Articleimage/2014/CC/c4cc03001j/c4cc03001j-f1_hi-res.gif

Material and Method Radish juice DI water Blend (10min, 2 times) Tea extract DI water Boiled and Filtered Preparation of plant extract

Material and Method Synthesis Ag/AgCl-Radish (Ag/AgCl-R) 20 min AgNO 3 NaCl Radish Juice Ag

Synthesis Ag-Radish (Ag-R) Material and Method 15 min AgNO 3 Radish Juice Ag

Synthesis Ag-Tea (Ag-T) Material and Method AgNO 3 Mixing Settle at RT Tea extracted Ag

Result and Discussion Characterization of Ag NPs by SEM Ag/AgCl-R Ag-C Ag-R Ag-T

Result and Discussion Characterization of Ag NPs AgNP Crystal size (nm) Particle size (nm) pH pzc Ag/AgCl-R68.4± ±17.92 Ag-R59.1± ± Ag-T65.5± ± Ag-C79.6± ± XRD patterns of synthesized AgNP Crystal size, particle size and pH pzc of various types of AgNP Ag-T Ag/AgCl-R Ag-R Ag-C

LB agar 10 8 cfu/ml Incubate 37˚C 24 h Shaking at 250 rpm Bacteria in LB medium Incubate at 37˚C overnight CFU Ag NPs LB agar Agar disc diffusion test Inactivation test Bacteria culture E. coli S. aureus

Result and Discussion Agar disc diffusion test Ag/AgCl-R Ag-C Ag-T Control Ag-RControl Ag-R Ag/AgCl-R Ag-C Ag-T Control Ag-R Control Ag-R Ag/AgCl-R AgNP Inhibition zone diameter (mm) E. coliS. aureus Control (SDW)0.0±0.0 Ag-C0.0±0.0 Ag-T6.50± ±0.0 Ag-R8.75±0.25 Ag/AgCl-R8.75 ± ±0.10

Ag/AgCl-R Ag-R Ag-C Ag-T Result and Discussion Antibacterial activity of AgNP (100 µg/mL)

Result and Discussion Ag-T Ag/AgCl-R Ag-R Antibacterial activity (N/No) of AgNP on E. coli using different concentrations as a function of time

Result and Discussion AgNP Concentration of AgNP (µg/mL) Inactivation Rate (µg/mL-min) Antibacterial efficiency (%) Ag/AgCl-R Ag-R Ag-T Comparison of inactivation rate and antibacterial efficiency of AgNP on E. coli using different concentration of AgNP

Conclusion The obtained AgNP from plant extracts were presented in a smaller size (good dispersion ability, less agglomeration) The AgNP from Radish extracted provided the highest percentage in inhibition of E. coli and S. aureus. The inactivation of E.coli using the AgNP was greater than that of S. aureus for all AgNP. The Ag/AgCl-R and Ag-R provided higher antibacterial activity more than Ag-T

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