Genus Compylobacter Helicobacter pylori Objectives Describe the general structure, biochemical and antigenic structure of Compylobacter Illustrate the.

Slides:



Advertisements
Similar presentations
Gram Negative Bacilli Enterobacteriaceae Family:
Advertisements

Slightly Curved Bacilli
IDENTIFIKASI BAKTERI OLEHSUDRAJAT FMIPA UNMUL 2009.
Escherichia coli.
Gram Negative Rods of the Enteric Tract
DIARRHOEAL DISEASES Causes of Over-indulgence in Chemical Long-term antibiotic Viral causes: # Rotavirus # Norwalk.
Microbe of the Week Mycobacterium marinum The aquarium or fish tank disease,first reported in 1962 Rare but important if not treated Living example-Karen.
Gram-Positive Bacilli Part Two
BY RANJEET RAMAN GRAM NEGATIVE BACILLI- MICRO {ST1]
Chapter 17 – Vibrio, Aeromonas, Plesiomonas, & Campylobacter MLAB 2434 – Clinical Microbiology Cecile Sanders & Keri Brophy-Martinez.
Vibrio cholerae.
Case Study 67 Pathogenic Bacteriology 2009
Gram negative rods VibrionaceaeVibrio. General charcters of Vibrionaceae Gram negative, curved, comma shaped bacilli Motile by single polar flagella Non.
Diagnostic microbiology lecture: 7 CAMPYLOBACTER Abed ElKader Elottol MSc. Microbiology
(And Campylobacter-like species)
Vibrionaceae and Aeromonadaceae. Classification – contain three medically important genera Vibrio Aeromomas Pleisiomonas All members of these families.
Biochemical Tests.
Peptic Ulcer Disease. Peptic ulcer  refers to erosion of the mucosa lining any portion of the G.I. tract.  It is defined as : A circumscribed ulceration.
Escherichia coli 0157: H7 AKA- E. Coli (0157: H7) Greg Jamieson.
Peptic Ulcer and Helicobacter pylori infection:
Clinical Microbiology (MLCM- 201) Prof. Dr. Ebtisam. F. El Ghazzawi. Medical Research Institute (MRI) Alexandria University.
Campylobacter. Campylobacter Campylobacter Comma shaped ,gram (-) rods, μm wide ₓ μm Motile by a polar flagellum Most species microaerophilic.
بسم الله الرحمن الرحيم GENUS: CAMPYLOBACTER Prof. Khalifa Sifaw Ghenghesh.
Campylobacter Dr. Abdulaziz Bamarouf
Campylobacter - Microscopic appearance - Cultural characteristics
CAMPYLOBACTER & Helicobacter Gram negative curved rods Gram negative curved rods Dr. H.Gh.Safaei.
Campylobacter: C. Jejuni and C. coli have emerged as common human pathogens, causing mainly enteritis and occasionally systemic diseases. Morphology: Small.
Campylobacter Spirally small, curved, G-ve, Motile, NS, NC bacteria.
Diseases of The Stomach Prof: Hussien Gadalla. Gastric Disorders Acute Gastritis Chronic Gastritis Peptic Ulcer Disease These three are common and related.
Peptic Ulcer Disease Dr. Wael H. Mansy, MD Assistant Professor College of Pharmacy King Saud University.
Enterobacteriaceae: They include large heterogeneous group of gram negative rods whose natural habitat is the intestinal tract of man or animals. General.
CampylobacteriaceaeCampylobacteriaceae Campylobacter Heliobacter Heliobacter (Gram-negative curved rods)
K. pneumoniae is most common pathogen K. pneumoniae is most common pathogen Pneumonia (nosocomial) or UTI (makes urease) Pneumonia (nosocomial) or UTI.
PowerPoint ® Lecture Slides for M ICROBIOLOGY Slightly Curved Bacilli.
Aerobic Non-Spore Forming Gram-Positive Bacilli
Enterobacteriaceae II
Role of H. pylori in Peptic Ulcer and drugs used in Treatment Dr. Fawzia Alotaibi.
Najran University College of Medicine Enterobacteriaecae 2 BY Dr. Ahmed Morad Asaad Professor of Microbiology.
Campylobacter. Campylobacter curved, spiral or S shaped organism Microscopically resemble vibrio Gram-negative microaerophilic, oxidase-positive, non-fermentative.
VIBRIO CHOLERAE CHOLERA. CHARACTERISTICS GRAM-NEGATIVE, COMMA SHAPED BACILLI MOTILE – POLAR FLAGELLA OXIDASE – POSITIVE CATALASE – POSITIVE ALKALNE PH.
Chapter 16 Vibrio section 1 Vibrio cholera Biological characterization A. Morphology and identification  Comma shaped, curved rod  G-  Single polar.
Vibrios, Campylobacters and Associated Bacteria
Gram Negative Bacilli Dr. Nahed Gomaa. True Bacteria Gm +ve Cocci Bacilli Gm -ve Cocci Neisseria gonorrhea Neisseria meningitidis Bacilli Pleomorphic.
Peptic Ulcer and Helicobacter pylori Infection. History: In 1983, Barry Marshall and Robin Warren, reported that H. pylori is associated with chronic.
BACILLARY DYSENTERY SHIGELLOSIS
SALMONELLA Important Gram-negative Lactose Produce H 2 S.
SALMONELLA.
THE GENUS HELICOBACTER Gram –ve rod, curved microaerophilic oxidase + strong urease activity At least 22 species included in this genus Majority colonize.
Objectives By the end of this lecture the student must be:
Helicobacter pylori and gastric ulcers. Helicobacter pylori (H. pylori) is a spiral shaped bacterium that lives on the lining of the stomach; inflammation.
Brucella Objectives Describe the general structure, biochemical, Antigenic structures and diagnostic criteria of Brucella. Illustrate the pathogenesis.
Department: Microbiology
Vibrio cholerae Vibrio is one of the most common bacteria in surface waters worldwide. They are curved aerobic rods and are motile, by polar flagellum.
Pseudomonas د.وليد خالد سعدون.
Gastritis.
Department: Microbiology
PHARMACEUTICAL MICROBIOLOGY -1I PHT 313
Genus Vibrio Objectives
Enterobacteriaceae Aim of these lectures
ENTEROBACTERIACEAE.
Aerobic Non-Spore Forming Gram-Positive Bacilli
Order: Pseudomonadales
Gastrointestinal pathogens: Helicobacter pylori
Gastrointestinal pathogens: Vibrio cholerae
Peptic Ulcer and Helicobacter pylori infection:
Asst. Prof. Dr. Dalya Basil
ENTEROBACTERIACEAE 1.
Practical No.17 Proteus & Pseudomonas.
Campylobacter Microbiology properties Curved (comma- or S-shaped)
Presentation transcript:

Genus Compylobacter Helicobacter pylori Objectives Describe the general structure, biochemical and antigenic structure of Compylobacter Illustrate the pathogenesis and clinical findings of enterocolitis caused by Compylobacter Describe the general structure and biochemical criteria of H.pylori Illustrate the pathogenesis and clinical findings of H.pylori infection List the lab diagnosis of H.pylori infection.

Genus Compylobacter - C. jejuni & C. coli ← emerged as common human pathogens. -Gram negative, comma,S or gall-wing shape rods, motile with single polar flagellum & non-spore forming -They are thermophilic (37oC – 42oC), grow on reduced O2 (0.5%) and 20% CO2 -The selective media is "Skirrows media" incorporated with vancomycin, polymyxin B & Trimethoprim -They are oxidase positive, catalase positive, urease negative, reduce nitrate and produce H2S, they do not ferment CHO. -Hippurate Hydrolysis test is used to differentiate between spp. The antigenic structure: 1-Heat stable lipopolysaccharide O-Ag with endotoxic activity. 2-Heat labile flagellar H-Ag.

Pathogenesis Domestic animals serve as source of the pathogens for humans. Transmission is usually feco-oral, (≥10000m.o.) The bacteria multiply in the small intestine → invading the epithelium & producing inflammation which result in appearance of RBC & WBC. Clinical findings: IP (1-7) days, acute onset begins as watery foul-smelling diarrhea followed by bloody stool accompanied by fever and sever abdominal pain. This enterocolitis usually self-limited (5-8) days.

Laboratory diagnosis: A-Specimen Diarrheal stool. Blood B-Culture on Skirrow's media → small, gray, circular and glistening colonies ← detected by Gram’s stain for typical morphology.

Helicobacter pylori

General characteristics 1-Gram negative, spiral shaped rods. actively motile with multiple polar flagella. 2-Culture on Skirrow’s media (3-6 days∕ 37 o C) 3-Oxidase positive, catalase positive and urease positive. Pathogenesis of H.pylori H.pylori acquired by ingestion, use to attach to epithelial surface where the pH (6-7) Produce protease that modifies the gastric mucosa reducing the ability of acid to diffuse through mucus. Produce potent urease activity that cause accumulation of large ammonia which buffer the acidity. Toxin and LPS may damage the mucosal cells.

Gastritis is characterized by chronic and active inflammation, even epithelial destruction and glandular atrophy may occur, thus H.pylori may be a major risk factor for gastric cancer. Clinical findings After short IP, patient will develop acute gastritis (abdominal pain, nausea, flatulence and bad smell breath) for 2 weeks but hypochlorhydria may persist leading to chronic gastritis, peptic ulcer, deudenal ulcer or high risk of gastric cancer. No bacteremia or disaminated disease. Complete cure observed after elimination of the organism, although some people can harbor the pathogens for years without ill effect.

Labroatory diagnosis of H.pylori *Speciment - Gastric biopsy - Blood (serological Ab) 1-Histological smear: Gastritis and peptic ulcer usually diagnosed by gastroscopy, the taken biopsy can be stained by Giemsa or special silver stain, those can show the curved or spiral organisms. 2-Culture of biopsy: usually use Skirrows media, it take 3-6 days in microaeropilic environment. 3-Urease test either: -In vitro urease test done by inoculating the biopsy material on urea agar media, urease enzyme will split urea within 2hr shifting the pH to alkaline changing the color of indicator (phenol red) from yellow to pink.

4-Serological test: the presence of serum IgG as anti-H.pylori Ab will reflect the infection but is of limited use in follow up. - Invivo (urea breath test) done when C 13 or C 14 labeled urea ingested by the patient  urease activity  labeled CO 2 will be generated and can be detected in patients exhaled breath.

Summary - C. jejuni & C. coli are thermophilic, Gram negative, comma,S or gall-wing shape rods, motile with single polar flagellum & non-spore forming, grow on "Skirrows media”. Transmission is usually feco-oral,(≥10000m.o.) → invading the epithelium & producing inflammation → self-limited watery foul-smelling bloody diarrhea. -H.pylori are Gram negative, spiral actively motile rods with multiple polar flagella. Cultured on Skirrow’s media. Produce protease and potent urease which aids its pathogenic effect causing gastritis, peptic or deudenal ulcer or high risk of gastric cancer. Labroatory diagnosis of H.pylori i.Histology and culture of gastric biopsy ii.In vivo and in vitro urease tests iii.Detection of serological anti H.pylori IgG