Proper Micropipette Technique and Slide Preparation Day 2 http://www.chanceglass.co.uk/?p=slides

Proper Micropipetting Technique http://www.allposters.com/-sp/Tissue-Culture-Micro-Pipette-Well-Plate-Simulation-Posters_i3971574_.htm Proper Micropipetting Technique Part 1

What is a Micropipette? http://www.alibaba.com/product-free/104253083/Pipette_Controller_Pipette_Micropipette_Pipet_Autopipette.html Micropipettes are pipets that scientists use to measure and dispense (give out) very precise and small amounts of liquids. Micropipettes have two main components: the body and the tip.

Parts of Micropipette Plunger – 3 Positions (Rest, 1st Stop, 2nd Stop) Volume Adjustment – The knob you use to adjust the volume Volume Readout – How much volume you are measuring Tip Attachment – Where the tip attaches Tip Eject Button – Button to eject tip SIDE FRONT http://www.di.uq.edu.au/sparqmicropipette It is important that with a micropipette, students have a 1 per group (or pair) and a box of tips to follow along with the power point as the teacher goes over the slides and demonstrates with a micropipette for students to see.

1 L = 1000 mL 1 mL = 1000 μL 4 7 2 9 μL UNITS Ones Place Thousands Place Hundreds Place Tens Place Ones Place

200 – 1000 μL pipette (blue tips) First RED number is in the thousands place of μL: 0350 μL 20 – 200 μL pipette (yellow tips) First number is in the hundreds place of μL: 095 μL 2 – 20 μL pipette (yellow tips) First number is in the tens place of μL; RED number is after the decimal: 02.5 μL 0.2 – 2 μL pipette (white tips) First number is in the ones place of μL; RED numbers are after the decimal: 0.50 μL http://www.di.uq.edu.au/sparqmicropipette

Proper Micropipetting Procedure Draw up the Liquid Dispense the Liquid Pick the correct micropipette and set the volume that you wish to obtain. Hold the micropipette upright at all times. Push the plunger down to the first position then put it in the liquid. SLOWLY release the plunger. With a filled micropipette, slowly push the plunger from rest to Position 3. Remove tip from the liquid. Place tip into the waste container. http://www.clker.com/clipart-eppendorf-closed-with-pellet.html

You have until ______ (insert time) Practice Time! http://wordartworld.blogspot.com/2009/10/practice-makes-perfect.html Complete Section 2.1 You have until ______ (insert time)

Preparing Slides Part 2 http://www.thermoscientific.com/ecomm/servlet/home?storeId=11152

What is the Purpose of a Microscope? “Micro-” – Small “-Scope” – To look at A microscope is an instrument for viewing what is small by magnifying it. First invented in 1590 in the Netherlands http://www.biologycorner.com/worksheets/microscope-advanced.html

Parts of a Microscope 3. Objectives 1. Eye piece (Ocular Lens) 4. Stage 2. Adjustment Knob 5. Illuminator http://www.microsporetech.com/images/cordlessmicro_lg.jpg

Parts of a Microscope - Definitions 3. Objectives – Different levels of magnification 1. Eye piece (Ocular Lens) – Lens used to view your specimen 4. Stage – Flat platform where slides are placed 2. Adjustment Knob – Focuses the slide under various magnifications 5. Illuminator – A steady light source which illuminates specimen http://www.microsporetech.com/images/cordlessmicro_lg.jpg

When using microscopes… Use TWO HANDS when carrying the microscope. Only use LENS PAPER when cleaning the lens and eyepieces. Always start with the LOWEST MAGNIFICATION. Lower the stage ALL THE WAY before placing the slide on the stage. Focus using the COARSE ADJUSTMENT knob first. http://www.securityworldnews.com/2011/02/10/safety-posters-for-your-workplace/

Slide Preparation – Materials http://www.ehow.co.uk/how_7644973_prepare-wetmount-slide-pond-water.html Slide Coverslip 2-5mm pieces of double stick tape Micropipette Liquid sample Nail polish (optional)

Slide Preparation – Procedure http://www.ehow.co.uk/how_7644973_prepare-wetmount-slide-pond-water.html Place 2-5 x 20 mm pieces of double sided tape on a slide spaced about 10 mm apart. Place the coverslip on top of the tape. Using a micropipette, place 100 μL of your liquid solution in the channel created. Place under the microscope. Observe. If students are not finished, you can have them seal their coverslips to their slides with clear nail polish to prevent the slide from drying out overnight.

You have until ______ (insert time) Practice Time! http://wordartworld.blogspot.com/2009/10/practice-makes-perfect.html Complete Section 2.2 You have until ______ (insert time)