APALIGHT 

APALIGHT Hexagonal structure (P63/m) Cell dimensions a=b=9,42 Å, c=6,88 Å d = 3.19 hardness 5 refraction index = Ca 5 (PO 4 ) 3 (OH, F, Cl, CO 3 ) HYDROXYAPATITE

APALIGHT One of the few minerals produced and used by biological micro-environmental systems It is the major component of teeth enamel and dentine (97% of the enamel and 70% of the dentine) It is the main component of bones (70%) Ca 5 (PO 4 ) 3 (OH, F, Cl, CO 3 ) HYDROXYAPATITE VERY SAFE

APALIGHT Prosthesis covering Semi-permanent filler in non-surgical options in skin rejuvenation Promotes bone growth in nutraceutical products Effective in increasing bones mineral density Promotes osseointegration HYDROXYAPATITE MEDICAL APPLICATIONS

APALIGHT It is a new skin-friendly mineral active, belonging to the hydroxyapatites group Our tests: 1.as an active in a series of evaluations related to the anti-ageing efficacy 2.as a booster of sun protection in SPF in vivo tests (enhancing significantly UV-B ray protection) APALIGHT

APALIGHT IN-VIVO TEST

APALIGHT ANTI-AGING EFFICACY ANTI-AGING EFFICACY 6% HA vs Placebo 10 vol. ( 4 weeks) 1. Skin Roughness 2. Skin Elasticity 3. rh

p < 0.05 p > 0.05 APALIGHT SKIN ROUGHNESS 1 - SKIN ROUGHNESS (ANTI-AGING EFFICACY) R z = Maximum Roughness - 9.5% APALIGHT  PLACEBO %∆ of roughness SEBUMETER SM 810 Courage & Khazaka + SKIN SURFACE REPLICA and surface analysis, Monaderm,Monaco

p = 0.05 p > 0.05 APALIGHT 2 - SKIN ELASTICITY (ANTI-AGING EFFICACY) R2 = Overall biological elasticity % APALIGHT  PLACEBO %∆ of elasticity CUTOMETER SEM 575, Courage & Khazaka +

APALIGHT SKIN ELASTICITY 2 - SKIN ELASTICITY (ANTI-AGING EFFICACY) R6 = Viscoelastic ratio % APALIGHT  PLACEBO %∆ of viscoelasticity CUTOMETER SEM 575, Courage & Khazaka

S5 / Tf APALIGHT 3 – SOFT FOCUS EFFECT S5 / T0 S10 / T0S10 / Tf

APALIGHT Biological Mineral Skin friendly Environmentally friendly Physical a new… Sun Protection Sun Protection Agent!

APALIGHT CURRENT SUN FILTERS SITUATION It is well known organic chemical filters could potentially produce harmful substances under UV irradiation Inorganic physical filters (TiO 2 ), used for years, are generally considered to be inert and safe. Anyway Ti0 2 may generate a conspicuous number of ROS in aqueous solutions

SUN PROTECTION FACTOR (SPF) APALIGHT Colipa Standard P3 SPF % TiO 2 Colipa Standard P3 SPF % HA Colipa Standard P3 SPF % HA Sunscreen SPF % TiO 2 Sunscreen SPF % HA Sunscreen SPF % TiO 2 Sunscreen SPF % HA METHOD COLIPA CTFA-SA JCIA and CTFA 2006

APALIGHT SUNSCREEN FILTERS % P3 SPF 15SPF 30SPF 50+ ETHYLHEXYL METHOXYCINNAMATE 310 ENSULIZOLE 2.78 ETHYLHEXYL SALICYLATE 44 ETHYLHEXYL TRIAZONE 1.53 DIETHYLAMINO HYDROXYBENZOYL HEXYL BENZOATE 710 BUTYL METHOXYDIBENZOYLMETHANE VERY POOR (UVB filters) (UVA filters)

APALIGHT SUNSCREEN EFFICACY (COLIPA P3)+ 6% TiO % HA+ 6% HA SPF (Sunscreen) + 4% TiO 2 + 4% HA SPF 3034,1040,30 - (Sunscreen) + 5%TiO 2 + 5% HA SPF ,00 66,20 - IN VIVO TESTED

APALIGHT SPF % SPF % SPF % Vs TiO 2 SUN PROTECTION FACTOR

EDTA 0,3 MICROCRYSTALLYNE CELLULOSE/CELLULOSE GUM 1,5 C12-15 ALKYL BENZOATE 5 CETYL ALCOHOL/GLYCERYL STEARATE/PEG-75 STEARATE/CETETH-20/STEARETH-20 5 POLYESTER-7 3 CETEARETH-25 0,25 DIMETHICONE 5 CYCLOPENTASILOXANE/DIMETHICONE VINYL DIMETHICONE CROSSPOLYMER 3 CYCLOPENTASILOXANE 3 ETHYLHEXYLMETHOXYCINNAMATE 7,5 BUTYL METHOXYDIBENZOYLMETHANE 3,0 OCTOCRYLENE 2,7 METHYLENE BIS-BENZOTRIAZOLYL TETRAMETHYLBUTYLPHENOL 9,9 APALIGHT further SUNSCREEN TESTS + 6% HA 30 SPF 30 Water as needed to reach 100% IN VIVO TESTED

EDTA 0,1 MICROCRYSTALLYNE CELLULOSE/CELLULOSE GUM 1,5 C12-15 ALKYL BENZOATE 5 CETYL ALCOHOL/GLYCERYL STEARATE/PEG-75 STEARATE/CETETH-20/STEARETH-20 5 POLYESTER-7 3 CETEARETH-25 0,25 DIMETHICONE 5 CYCLOPENTASILOXANE/DIMETHICONE VINYL DIMETHICONE CROSSPOLYMER 3 CYCLOPENTASILOXANE 3 ETHYLHEXYLMETHOXYCINNAMATE 7,0 BUTYL METHOXYDIBENZOYLMETHANE 3,0 OCTOCRYLENE 2,7 APALIGHT further SUNSCREEN TESTS + 6% HA 15 SPF 15 Water as needed to reach 100% IN VIVO TESTED

APALIGHT SPF 15SPF 17, % SPF 30SPF 40, % SUN PROTECTION FACTOR

APALIGHT IN VIVO AN IN VIVO TESTED EFFICACY

SUN PROTECTION FACTOR (SPF) APALIGHT Sunscreen 20% ZnO + 5% ZnO ZnO: Zano 10 plus (Ultra Chemical Inc.) UVA/UVB protection coated with triethoxycaprylylsilane. Sunscreen 20% ZnO + 5% HA Same ZnO + Apalight METHOD ISO 24444:2010 ONLY PHYSYCAL FILTERS (SPF16) 20 ZnO + 5% ZnO 20 ZnO + 5% HA SPF20,420,7

APALIGHT more features…

APALIGHT NO BLUE WHITE APPEARANCE

Is a physiological ingredient Is able to enhance UV protection Its performances are comparable or even superior to those of Titanium dioxide No blue-white appearance Antiage effect: Elasticity, Antiwrinkle, Roughness Lower cost of formula vs TiO 2 For Sun Care products or day cream APALIGHT CONCLUSION APALIGHT 

APALIGHT pH 7÷8 Microfine white stable suspension (28÷30% solid) or powder Around 100 nm but not nanoparticle (Cosmos) Special ionic composition FEATURES

APALIGHT Via Pastore Botticino Sera (Brescia) Italy Tel Fax Thank you