Lab-on-a-Chip The Ideal Technology for Bio-chemical Analysis

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Lab-on-a-Chip The Ideal Technology for Bio-chemical Analysis Xingyu Jiang Ph.D. xingyujiang@nanoctr.cn National Center for Nanoscience and Technology, China

What is “Lab-on-a-chip”? A lab-on-a-chip (LOC) is a device that integrates one or several laboratory functions on a single chip of only millimeters to a few square centimeters in size.

What can “Lab-on-a-chip” do? Biochemical assays: real-time PCR, immunoassay, dielectrophoresis for detecting cancer cells and bacteria, etc. Chemical application: separating molecules from mixtures, chemical reactors, chemical detections. etc. Biological application: cell coculture, biosensor, drug screening, single-cell analysis, etc.

Automation for Lab-on-a-chip Development of Microfluidic Large Scale Integration with thousands of mechanical valves Parallel Assays Made Easy!

Microfluidic Diagnostic Methods Portable Point-of-Care (POC) Medical Diagnostic Systems The chips should be inexpensive, accurate, reliable and suited to the medical conditions in developing countries.

How to Prepare a Microfluidic Chip? Photolithography or etching L-Edit, AutoCAD Print Soft embossing

The Method for Preparing a Chip in Our Lab: Soft embossing by polymer (PDMS etc.)

A method for analyzing HIV antibodies in microfluidic channels PDMS:Polydimethylsiloxane PC: Polycarbonates

Improved efficiency in assays Antigens Antibodies Immunoassay results on PC membrane Advantages: Fast (20 min.) High throughput Low cost

Micro Western blot for detecting multiple proteins on one chip Detection of multi-proteins one time 1-3µL Antibody solutions are required

Loop-mediated isothermal amplification (LAMP) integrated on microfluidic chips for detection of pathogen Jiang, et al, Anal. Chem. 2010,82, 3002

Cells on a Chip

Microfluidic technology in cell coculture We developed a method for patterning multiple types of adherent cells on the same substrate.

Naked-Eye Based Assays

Nanotechnology in chemical analysis