Biosensor using aptamer. Contents  What is biosensor?  What is aptamer?  How can we use aptamer?  How can we find aptamer?

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Presentation transcript:

Biosensor using aptamer

Contents  What is biosensor?  What is aptamer?  How can we use aptamer?  How can we find aptamer?

What is Biosensor? Analytical device using biological element Sensing organic molecule’s state or concentration

Biomarker of Heart attacks

What is aptamer? Piece of single strand DNA or RNA High affinity for certain molecule Used for bioreceptor of biosensor

Small size  easy production, modification High stability  easy to handle, regeneration Economically efficient No immune rejection response Adjust to many toxic material or complex protein

How can we find aptamer? vitro selection SELEX(Systematic Evolution of Ligands by Exponential Enrichment)

Different kind of SELEX  Cell SELEX  Capillary electrophoresis SELEX (CE SELEX)  Speigelmer

How can we use aptamer?  Sensor  Diagnosis  Cure

Discussion  Where can aptamer be used in another way?

Reference  장승훈, 정기준, 압타머 (Aptamer) 개발의 최근 연구 동향, 한 국과학기술원 생명화학공학과  Li Cai, Ze-Zhong Chen, Min-Yan Chen, Hong-Wu Tang*, Dai-Wen Pang, MUC-1 aptamer-conjugated dye- doped silica nanoparticles for MCF-7 cells detection, 2013, Biomaterials

Thank you!!