Quad Rat Vitals Monitor Team: Jack Ho Kuya Takami Nathan Werbeckes Joseph Yuen Client: Dr. Alex Converse Advisor: Dr. Tom Yen.

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Presentation transcript:

Quad Rat Vitals Monitor Team: Jack Ho Kuya Takami Nathan Werbeckes Joseph Yuen Client: Dr. Alex Converse Advisor: Dr. Tom Yen

Problem Statement Vitals for anesthetized rats during PET imaging – SpO 2 (±2%) – Heart rate ( bpm) – Rectal temperature (93 – 100° F) – Respiration rate (20-30 bpm) Current oximeter is designed for “small animals” – Inaccurate at faster heart rates Current devices too expensive – Budget = $4,000 USD Problem Statement : Background Info : Strategy : Future Work Jack Ho Team Leader

Quad Adjustable Isoflurane Delivery * O2O2 Isoflurane Rat *Slide by Dr. Alex Converse Problem Statement : Background Info : Strategy : Future Work Jack Ho Team Leader

Pulse Oximeter Red and infrared LED and photodiode Absorption is different between oxyhemoglobin and its deoxygenated form Oxygen absorption can calculated from ratio Measures heart rate Mostly for humans Nellcor, Criticare, Nonin, etc. Problem Statement : Background Info : Strategy : Future Work Joseph Yuen BSAC

Current Devices Veterinary Oximeters – Nonin, Bionet, etc. Starr Life Sciences – MouseOx Expensive Problem Statement : Background Info : Strategy : Future Work Joseph Yuen BSAC

Buy pulse oximeter built for rats/mice Buy sensors designed for rats/mice Build a multiplexor DesignCostConstructionDesign/planningAdaptable 4 rats # Design 1: Buy Problem Statement : Background Info : Strategy : Future Work Nate Werbeckes BWIG

Build sensor designed for rat’s paw Build filters, comparators, differential amps, etc. to compute pulse/ox Convert to digital signal and input through USB DesignCostConstructionDesign/planningAdaptable 4 rats # Design 2: Build Problem Statement : Background Info : Strategy : Future Work Nate Werbeckes BWIG

Reverse engineer human sensor Split wires and plug into data acquisition box Code pulse/ox computation in LabVIEW DesignCostConstructionDesign/planningAdaptable 4 rats # Design 3: Adapt Problem Statement : Background Info : Strategy : Future Work Nate Werbeckes BWIG

Design Matrix DesignCostConstructionDesigning/PlanningAdaptable4 rats Buy Build91158 Adapt67799 Weights DesignCostConstructionDesigning/PlanningAdaptable4 ratsTotal Buy Build Adapt Problem Statement : Background Info : Strategy : Future Work Nate Werbeckes BWIG

Flow Chart of LabVIEW processes Red + IR + Ground Red - Ground IR - Ground Red/IR Graph Detect increasing edges Calculate SpO 2 Calculate time in between edges Convert to bpm Display SpO 2 Display bpm Problem Statement : Background Info : Strategy : Future Work Nate Werbeckes BWIG

Future Work Problem Statement : Background Info : Strategy : Future Work Kuya Takami Communicator Computer LabView Computer LabView DAq Box Pulse/Ox Sensor Isoflurane & O 2 Control Thermometer Respiratory Rate

References Webster, J. G., Design of Pulse Oximeters. IOP Publishing Ltd D. Ford, D. Nachreiner, R. Thomas, “Design of a Pulse Oximeter for Use in Mice”, 2005 Starr Life Sciences, (website).

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