Data summary – “alleles” Threshold Over threshold, peaks are labeled as allele events All-or-none allele peaks, each given equal status Allele Pair 8,

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Presentation transcript:

Data summary – “alleles” Threshold Over threshold, peaks are labeled as allele events All-or-none allele peaks, each given equal status Allele Pair 8, 8 8, 11 8, 12 8, 14 11, 11 11, 12 11, 14 12, 12 10%12, 14 14, 14

Probability of inclusion (PI) Simple formula: For all "alleles" over threshold, add up their frequencies, and square the number ( ) 2 = (.765) 2 =.585 Threshold match statistic is 1/PI 1/(.585) = 1.71 Computer match statistic is 35 Calculation at locus D13S317

Match statistic comparison DNA locus Likelihood ratio (LR)

Information comparison Jeans 280 thousand (5) 2 quadrillion (15) T-shirt 630 thousand (5) 4 quadrillion (15) Method Combined PI TrueAllele

Validated genotyping method Perlin MW, Sinelnikov A. An information gap in DNA evidence interpretation. PLoS ONE. 2009;4(12):e8327. Ballantyne J, Hanson EK, Perlin MW. DNA mixture genotyping by probabilistic computer interpretation of binomially-sampled laser captured cell populations: Combining quantitative data for greater identification information. Science & Justice. 2013;53(2): Perlin MW, Hornyak J, Sugimoto G, Miller K. TrueAllele ® genotype identification on DNA mixtures containing up to five unknown contributors. Journal of Forensic Sciences. 2015;in press. Greenspoon SA, Schiermeier-Wood L, Jenkins BC. Establishing the limits of TrueAllele ® Casework: a validation study. Journal of Forensic Sciences. 2015;in press. Perlin MW, Legler MM, Spencer CE, Smith JL, Allan WP, Belrose JL, Duceman BW. Validating TrueAllele ® DNA mixture interpretation. Journal of Forensic Sciences. 2011;56(6): Perlin MW, Belrose JL, Duceman BW. New York State TrueAllele ® Casework validation study. Journal of Forensic Sciences. 2013;58(6): Perlin MW, Dormer K, Hornyak J, Schiermeier-Wood L, Greenspoon S. TrueAllele ® Casework on Virginia DNA mixture evidence: computer and manual interpretation in 72 reported criminal cases. PLOS ONE. 2014;(9)3:e92837.

Preserves more match information TrueAllele CPI Perlin MW, Legler MM, Spencer CE, Smith JL, Allan WP, Belrose JL, Duceman BW. Validating TrueAllele ® DNA mixture interpretation. Journal of Forensic Sciences. 2011;56(6):

Preserves more DNA evidence Perlin MW, Belrose JL, Duceman BW. New York State TrueAllele ® Casework validation study. Journal of Forensic Sciences. 2013;58(6):

CPI information CPI million Combined probability of inclusion Perlin MW, Dormer K, Hornyak J, Schiermeier-Wood L, Greenspoon S. TrueAllele ® Casework on Virginia DNA mixture evidence: computer and manual interpretation in 72 reported criminal cases. PLOS ONE. 2014;(9)3:e92837.

MIX05: Thresholds not reproducible National Institute of Standards and Technology Two Contributor Mixture Data, Known Victim 31 thousand (4) 213 trillion (14)

SWGDAM 2010 guidelines Threshold Higher threshold for human review Under threshold, alleles less used Allele Pair 8, 8 8, 11 8, 12 8, 14 11, 11 11, 12 11, 14 12, 12 0%12, 14 14, 14

Modified CPI information CPI million mCPI

MIX13: An interlaboratory study on the present state of DNA mixture interpretation in the U.S. Coble M, National Institute of Standards and Technology 5th Annual Prescription for Criminal Justice Forensics, Fordham University School of Law, MIX13: Thresholds falsely include