Compound Light Microscope Becky Sheridan

Compound (2) Light Microscope Two Lenses Objective Lens Ocular/Eyepiece Lens Total Magnification Both lenses multiplied Multiply the Objective Lens (#) x Eyepiece Lens (#) = Total Magnification Example: 10 x 10 = 100TM

What does the microscope do to an image? “e” Makes it bigger Turns it upside down Turns it backwards Shows more detail Low Power High Power

What happens when you move an object under the microscope? Away Towards you Left Right Move toward you Moves away Moves right Moves left For a tutorial on the Compound Light Microscope Author's): Jen Klipfel, Nabila Jahchan, Peggy Klipfel LeDuff Go to: http://www.csun.edu/scied/7-microscopy/micro_tutorial/index.html

VS Resolution The ability to see two items as separate discrete units. Magnification The size of an image

http://homepages.gac.edu/~cellab/chpts/chpt1/figure1-1.html

http://www. sciencelearn. org http://www.sciencelearn.org.nz/var/sciencelearn/storage/images/contexts/exploring-with-microscopes/sci-media/images/resolving-power-of-microscopes/639092-3-eng-NZ/Resolving-power-of-microscopes.jpg

Refraction and Immersion Oil Refraction: the bending of light as it passes from one medium to another of different density. This is due to differences in their index of refraction. Ex. Light passing through a glass microscope slide, air and then lens is refracted each time. This may result in blurring of the image when using the oil immersion lens. Immersion Oil: the refractive index of immersion oil is about 1.518 which is close to that of glass

Refractive Index of Glass & Immersion Oil Refractive Index of Glass & Immersion Oil. The glass rod placed in immersion oil is practically invisible while the rod in water is visible. Immersion oil has the same refractive index as glass, therefore light passing thru the oil & glass is not bent concealing the surface of the glass. Immersion Oil Water

Stereomicroscope Dissecting microscope

Stereomicroscope Dissecting microscope A microscope with a relatively low magnification. Good for viewing large objects Produces a 3-D image Advantages: it has a large depth of field, so you can observe thick objects with most parts in focus at the same time. can illuminate your sample using incident light as well as transmitted light, so that thick objects which will not transmit much light can be illuminated adequately to view.

Stereomicroscope the stage is much farther from the objective lens, allowing large objects to be placed on the stage. Hornet

Electron Microscopes Use a beam of electrons as the source of illumination allowing much greater magnification and resolution than a compound microscope. Transmission electron microscopes (TEM) transmit electrons through a thin slice of sample. Can view things like organelles in a cell. Scanning electron microscopes (SEM) use incident electrons on whole samples to get a 3-dimensional image. Images are viewed through an eyepiece, or projected onto a screen or photographic plate

TEM SEM Baby Bee Cell

TEM SEM