Stage II Stage VII Stage V Stage XII

2-Bromopropane (mg/kg/day) Days on test0 1,000 Day ±32.75 a 397.1±20.23 Day ± ±19.66 Day ± ±19.29** Day ± ±17.24** a Mean±SD (n=10); ** P<0.01 vs control Table 1. Body weight changes of male rats treated with 2-BP for 2 weeks

Table 2. Reproductive organ weights of male rats treated with 2-BP for 2 weeks 2-Bromopropane (mg/kg/day) Parameters0 1,000 Testis: left (g) per body weight (%) 1.77±0.119 a 0.42± ±0.064** 0.41±0.024 Testis: right (g) per body weight (%) 1.77± ± ±0.069** 0.42±0.018 Epidydimis: left (g) per body weight (%) 0.66± ± ± ±0.010 Epidydimis: right (g) per body weight (%) 0.68± ± ± ±0.013 Prostates (g) per body weight (%) 0.73± ± ± ±0.037 Seminal vesicles (g) per body weight (%) 1.30± ± ±0.122** 0.29±0.034 a Mean±SD (n=10); ** P<0.01 vs control

Table 3. The incidence and severity of histopathological lesions of testis and epididymis in male rats Control Days on test (1,000mg/kg/day) Day 3 Day 7 Day 14 Parameters Grades: Testis Atrophy of seminiferous tubules Exfoliation of spermatogenic cells Degeneration of spermatogonia Decrease of spermatogonia Degeneration of spermatocytes Decrease of spermatocytes Multinuclear giant cells Mature spermatid retention Vacuolation of Sertoli cells Epididymis Oligozoospermia Degenerated spermatogenic cells Grades are as follows: 0, normal; 1, slight; 2, moderate; and 3, severe change. N=10

* CD B * * C ESt RSt PSc SC Sg A Stage V

* * MSt C * B D A ZSc Sg SC PSc ESt Stage X

Table 4. The number of spermatogenic cells in seminiferous tubules of male rats Days on test (1,000 mg/kg/day) ParametersControlDay 3 Day 7 Day 14 StageSpermatogonia20.2±3.61 a 4.0±3.65**1.9±1.60**2.3±3.22** Ⅱ Pachytene spermatocytes49.4± ± ± ±7.47** Round spermatids158.3± ± ± ±16.09 Elongated spermatids153.5± ± ± ±39.73 Sertoli cells18.8± ± ± ±2.02 StageSpermatogonia37.4± ±3.89**2.1±2.13**2.0±3.62** Ⅴ Pachytene spermatocytes52.5± ± ± ±16.30* Round spermatids153.6± ± ± ±23.39 Elongated spermatids167.3± ± ± ±29.04 Sertoli cells17.5± ± ± ±2.42 a Mean±SD (n=10); *, ** P<0.05, P<0.01 vs control

Days on test (1,000 mg/kg/day) ParametersControlDay 3 Day 7 Day 14 StageSpermatogonia2.3±0.67 a 2.1± ± ±0.74 Ⅶ Preleptotene spermatocytes38.8± ± ±2.05**4.2±5.65** Pachytene spermatocytes56.4± ± ± ±9.83 Round spermatids151.6± ± ± ±31.61 Elongated spermatids157.0± ± ± ±25.72 Sertoli cells17.8± ± ± ±3.27 StageSpermatogonia3.6± ± ± ±1.51 Ⅻ Zygotene spermatocytes46.9± ± ±25.07**2.4±5.23** Pachytene spermatocytes61.8± ± ± ±19.16 Elongated spermatids168.7± ± ± ±27.83 Sertoli cells19.7± ± ± ±3.38 a Mean±SD (n=10); *, ** P<0.05, P<0.01 vs control Table 4. Continued

D * * * C A SC Sg PSc ESt RSt Stage II B