거핵구 (Megakaryocytic)

Thrombocyte(Platelets )

Platelet Satelitism ( 혈소판 위성현상 ) EDTA Pseudothrombocytopenia

VIII Collagen binding site Adhesion site Aggregation, vWF and fibrinogen binding site GpIIb GpIIIa GpIb GpIa Platelet membrane Subendothelium(collagen myofibrils) vWF 1) Adhesion ( 점착능 )

2) ( 응집능 )

Aggregation test Platelet Function Test

(min) 광선투과율 (%) PPP PRP 방출이상 혈소판무력증 EPI (3mM) 정상정상 정상 경증 vWD 중증 vWD Ristocetin (1.2ug/ml) ADP (3uM) 혈소판무력증 방출이상 정상 방출이상 정상 혈소판무력증 Collagen (1:200) (min)

, ATP, Serotonin, Ca vWF 3) ( 방출능 )

정상 Normal 저하 Decreased 불량 Poor 4) Clot retraction ( 혈병수축능 )

5) Coagulation support 5) Coagulation support

Platelet Function Test BT (Duke)

40mmHg BT (Ivy) Platelet Function Test

모세혈관 저항성검사 (Rumpel-Leede tourniquet test) 80mmHg Platelet Function Test

INTRINSIC PATHWAY … CT, RECALCIFIED CT, APTT (XII, XI, IX, VIII) EXTRINSIC PATHWAY … PT (VII) COMMON PATHWAY … TT (I)

Platelet Function Analyzer-100 COL/EPI cartridges with pipetting of blood sample in PFA-100 Collagen-Epinephrine cartridges

Cartridge plug formation aperture  150 µm collagen + EPI or ADP capillary  200 µm membrane sample 800 µl  Start platelet plug End of test

Development of plug closure 15 sec 45 sec 80 sec 110 sec

Coagulation groups 1) Fibrinogen group (I, V, VIII, XIII) 2) Prothrombin group (II, VII, IX, X) - BaSO 4 absorbed plasma ?? - Vitamin K dependent - Serine protease 3) Contact group (XII, XI, HMWK, Prekalikrein)

INTRINSIC PATHWAY … CT, RECALCIFIED CT, APTT (XII, XI, IX, VIII) EXTRINSIC PATHWAY … PT (VII) COMMON PATHWAY … TT (I)

Coagulation time (capillary method) Coagulation time (Lee-White method) Intrinsic pathway 8mm 시험관 => 5-8 분 11mm 시험관 => 6-12 분

3) aPTT 2) Recalcified CT 1) CT 4) Factor 8 assay