MICROBIOLOGIA GENERALE Viral replication

Possible cytopathogenesis of cells infected with animal viruses

The reproductive cycle of animal viruses Virus attachment to host cell Virus entry into cells Transcription, translation and genome replication Assembly, exit and maturation of progeny virions

ATTACHMENT PENETRATION VIRAL SYNTHESIS UNCOATING ASSEMBLY EXIT TRANSCRIPTION UNCOATING TRANSLATION REPLICATION ASSEMBLY (MATURATION) EXIT

virus attachment to host cells Viral replication virus attachment to host cells

Viral receptors and coreceptors

Influenza virus structure

virus entry into host cells Viral replication virus entry into host cells

Three entry and uncoating strategies

Mechanism of uncoating within endosomes

Virus entry via receptor-mediated endocytosis

transcription, translation and genome replication Viral replication transcription, translation and genome replication

Genomes of DNA viruses unimolecular ds or ss 5-240 kb linear or circular Genomes of RNA viruses unimolecular or segmented ss or ds 1,7-25 kb linear or circular (+ )or (-) polarity

The diversity of viral transcription strategies Synthesis of viral macromolecules The diversity of viral transcription strategies The diversity of viral translation strategies The diversity of viral genome replication strategies

The diversity of viral transcription and translation strategies: Rhabdoviruses (-) ssRNA

The diversity of viral translation strategies: Picornaviruses polyprotein synthesis

The diversity of viral genome replication strategies: adenovirus DNA replication

The Baltimore classification system Class I and VII ds DNA

The Baltimore classification system Class I and VII ds DNA Class II ss DNA

The Baltimore classification system Class I and VII ds DNA Class II ss DNA Class III ds RNA (+/-)

The Baltimore classification system Class I and VII ds DNA Class II ss DNA Class III ds RNA (+/-) Class IV ss RNA (+)

The Baltimore classification system Class I and VII ds DNA Class II ss DNA Class III ds RNA (+/-) Class IV ss RNA (+) Class V ds RNA (-)

The Baltimore classification system Class I and VII ds DNA Class II ss DNA Class III ds RNA (+/-) Class IV ss RNA (+) Class V ds RNA (-) Class VI

The Baltimore classification system Parvovirus (B19) Class I and VII ds DNA Class II ss DNA Class III ds RNA (+/-) Class IV ss RNA (+) Class V ds RNA (-) Class VI Adenovirus Herpesvirus Papilloma Polyomavirus Poxvirus Hepadnavirus Retrovirus Orthomyxovirus Paramyxovirus Rhabdovirus Reovirus Picornavirus Togavirus

transcription, translation and genome replication of DNA viruses Viral replication transcription, translation and genome replication of DNA viruses

Flow of events in multiplication of Herpeviruses: an example Class I virus Figure: 16-20 Caption:

Structure and genome organization of Herpes simplex 1 virus (HSV-1)

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

Structure and genome organization of the Poxvirus Vaccinia virus: an example of Class I virus that replicates in the cytoplasm

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

transcription, translation and genome replication of RNA viruses Viral replication transcription, translation and genome replication of RNA viruses

V IV III

Structure and genome organization of the Picornavirus Poliovirus: an example of Class IV virus

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

Poliovirus RNA replication

Structure and genome organization of the Rhabdovirus Vesicular Stomatitis Virus: an example of Class V virus

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

The Orthomyxovirus Influenza A virus: an example of Class V virus that replicates in the nucleus

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

Class VI virus: the Retroviruses

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

Replication process of a Retrovirus

Replication process of a Retrovirus

Translation of Retrovirus mRNA and processing of the proteins: the GAG and POL proteins

Translation of Retrovirus mRNA and processing of the proteins: the ENV proteins SU TM

Structure and genome organization of the Class VII virus: Hepadnaviruses

Attachment Entry Uncoating Viral Synthesis Assembly Maturation Exit

assembly, exit and maturation of progeny virions Viral replication assembly, exit and maturation of progeny virions

Hypothetical pathway of virion assembly and release

Assembly of Poliovirus in the cytoplam of an infected cell

Assembly of a Retrovirus from polyprotein precursors

Mechanism of budding of enveloped viruses