AllPure Technologies aseptic sampling system

© 2011 llPure ® Simplified sampling Precision controlled sampling Effortless protection against contamination Factory assembled Gamma irradiated aseptic sampling

© 2011 llPure ® What are you measuring? pH pO 2 pCO 2 Osmolality Air Saturation Total CO 2 CO 2 HCO 3 Sodium Potassium Cell Viability Cell Density Ammonium Glucose Lactate Glutamine Glutamate Glycerol does it all

© 2011 llPure ® Which is right for you? integrated purge with QUICK SEAL ® dedicated purge shared purge without QUICK SEAL ® integrated purge dedicated purge shared purge Click to explore product features

© 2011 llPure ® swabbable needleless access site multi-access sampling site aseptically connect syringe up to 140 times one-way check valve effortless protection against contamination QUICK SEAL ® aseptic disconnect secure & reliable sterile disconnect C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Recommended Procedure 1.1 Weld onto bioreactor 2.1 Wipe access site with alcohol 3.1 Engage syringe for purge and clear line 4.1 Disengage purge syringe 5.1 Engage syringe for sample and collect sample Click mouse to advance slide

© 2011 llPure ® Recommended Procedure 1.1 Weld onto bioreactor 2.1 Wipe access site with alcohol 3.1 Engage syringe for purge and clear line 4.1 Disengage purge syringe 5.1 Engage syringe for sample and collect sample swabbable needleless access site multi-access sampling site aseptically connect syringe up to 140 times one-way check valve effortless protection against contamination C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Click mouse to advance slide

© 2011 llPure ® swabbable access site multi-access sampling site aseptically connect syringe up to 140 times swabbable access site on stopcock multi-access sampling site aseptically connect syringe up to 140 times divert flow between sample & purge one-way check valve (2) effortless protection against contamination QUICK SEAL ® aseptic disconnect secure & reliable sterile disconnect C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Recommended Procedure 1.1 Weld onto bioreactor 2.1 Wipe access site on purge line with alcohol 3.1 Divert flow & engage syringe for purge and clear line 4.1 Wipe access site on sample line with alcohol 5.1 Divert flow & engage syringe for sample and collect sample Click mouse to advance slide

© 2011 llPure ® swabbable access site multi-access sampling site aseptically connect syringe up to 140 times swabbable access site on stopcock multi-access sampling site aseptically connect syringe up to 140 times divert flow between sample & purge one-way check valve (2) effortless protection against contamination C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Recommended Procedure 1.1 Weld onto bioreactor 2.1 Wipe access site on purge line with alcohol 3.1 Divert flow & engage syringe for purge and clear line 4.1 Wipe access site on sample line with alcohol 5.1 Divert flow & engage syringe for sample and collect sample Click mouse to advance slide

© 2011 llPure ® purge bottle integral molded cap 250mL PETG with vent filter swabbable access site on stopcock multi-access sampling site aseptically connect syringe up to 140 times divert flow between sample & purge one-way check valve (2) effortless protection against contamination QUICK SEAL ® aseptic disconnect secure & reliable sterile disconnect C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Recommended Procedure 1.1 Weld onto bioreactor 2.1 Divert flow for purge into bottle 3.1 Wipe access site on sample line with alcohol 4.1 Divert flow & engage syringe for sample and collect sample Click mouse to advance slide

© 2011 llPure ® purge bottle integral molded cap 250mL PETG with vent filter swabbable access site on stopcock multi-access sampling site aseptically connect syringe up to 140 times divert flow between sample & purge one-way check valve (2) effortless protection against contamination C-Flex ® thermoplastic elastomer tubing ⅛” ID x ¼” OD sterile weld to bioreactor system Recommended Procedure 1.1 Weld onto bioreactor 2.1 Divert flow for purge into bottle 3.1 Wipe access site on sample line with alcohol 4.1 Divert flow & engage syringe for sample and collect sample Click mouse to advance slide

© 2011 llPure ® Swabbable Access Site 1. Align luer fitting on syringe 2. Luer tip seals against blue silicone ‘valve’ 3. Screw-on Luer to open valve 4. Fluid pathway is open Validated Microbial Barrier 140 actuations no contamination

© 2011 llPure ® One-way Check Valves USP Class VI Silicone diaphragm Operate in any orientation 87psi (6 bar) back pressure Effortless protection against contamination