Maximum Ingredient Level Optimization Workbook MIOW User Guide Rashed A. Alhotan, Graduate Student Dmitry V. Vedenov, Associate Professor Gene M. Pesti,

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Presentation transcript:

Maximum Ingredient Level Optimization Workbook MIOW User Guide Rashed A. Alhotan, Graduate Student Dmitry V. Vedenov, Associate Professor Gene M. Pesti, Professor

Overview of MIOW Workbook

Step 1:Design the experiment being simulated by making changes in cells C5:C6 & C8:C9 (Levels & Reps Worksheet) ❶ The desired number of ingredient levels should be typed in here (cell C5) ❷ The desired number of experimental replications should be typed in here (cell C6) ❸ Specify the min and max levels of the ingredient here ❹ Click it when finished designing the experiment to create the experimental grid ❺ The experimental grid: a combination of the replications and ingredient levels specified

Step 2:Select baseline model and type in the regression coefficients of the selected model (Simulations Worksheet) ❶ Select one model by clicking on it ❷ Type in the maximal biological response ❸ Decide the optimal value of the rate constant for the fitted function ❹ Type in the level that produces the maximal biological response in ❷ For the 2 end Order Polynomial, the Constant, Linear and Quadratic terms should be β o, β 1 and β 2 for the equation of the form y= β o +β 1 x+β 2 x 2 +ϵ

Step 3:Provide guesses for regression coefficients for the desired models (Simulations Worksheet) ❶ Select up to 3 models to show the results for ❷ Provide guesses for regression coefficients

Step 4:Select simulation parameters and run simulations (Simulations Worksheet) Step 1: Model selected in step 2 is displayed here ❷ Provide a value for the CV of the simulations ❶ Specify the number of simulated experiments (simulations) ❸ Click it to produce the results

Step 5: Reading the results (Simulations Worksheet) Step 1: The simulation results for the models selected in step 3 Max/Min = Maximum or Minimum biological response; MSL= Maximum Safe Level of the Ingredient; SE of MSL= Standard error of the mean MSL Each line represents one experiment For the Broken Line Model under the current settings The MSL= % ± (SD) or (SE) The confidence interval for the mean MSL is The coefficient of determination (R 2 ) is 98.8% Each value is the average of the experiments

Step 5: Reading the results (Simulations Worksheet) Step 1:

Step 6: View the Calculations Worksheet Step 1: The regression coefficients from step 2 Estimated regression coefficients for the current model levels and replications combinations