High Performance Liquid Chromatography Allyson Lundberg University of Utah November 29, 2006

Overview Introduction Theory Results Conclusions/Recommendation Questions

Applications Identification Separations Purification

Introduction Sugar analysis of Fructose and Sucrose

Theory Normal Phase Reversed Phase Size Exclusion Ion Exchange

Theory Column Selection –The Luna 5 micrometer Amino Column

Theory Sample Chromatograph

Theory Sucrose Calibration

Theory Fructose Calibration

Results Unknown Chromatograph

Results Sucrose Concentration 55 +/- 19 g/L (with 95% confidence) Fructose Concentration 186 +/- 22 g/L (with 95% confidence)

Conclusions and Recommendations Take precautions to make more precise calibration standards Perform more calibration runs with more standard solutions Perform more unknown runs

Summary Many applications for HPLC HPLC Theory Experimental Results Conclusions and Recommendations

References The Essential Guide to Analytical Chemistry. Schwedt, Georg. Wiley and Sons, Hoboken, NJ Analysis of Data. Haile, J. M. Macatea Productions, Central SC, CRC Handbook of Chemistry and Physics, 49 th College Edition. Edited by Weast, Robert C. The Chemical Rubber Company, Cleveland, OH, 1968 “High Performance Liquid Chromatography,” Unknown author, November 23,

Questions?